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CKM elisa kit :: Mouse creatine kinase, muscle ELISA Kit

Scan QR to view Datasheet Catalog #    MBS908119
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

creatine kinase, muscle (CKM), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Creatine kinase M-type, CKM ELISA Kit

 Product Synonym Names    Mouse Creatine kinase M-type (CKM) ELISA kit; CKMM; M-CK; creatine kinase M chain; creatine kinase-M; muscle creatine kinase; creatine kinase; muscle
 Product Gene Name   

CKM elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS908119 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P07310
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse CKM. No significant cross-reactivity or interference between mouse CKM and analogues was observed.
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 Samples    Serum, plasma
 Assay Type    Quantitative Sandwich
 Detection Range    1.56 mU/ml -100 mU/ml
 Sensitivity    Typically less than 0.39 mU/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CKM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CKM purchase    MBS908119 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the creatine kinase, muscle (CKM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56 mU/ml -100 mU/ml in biological research samples containing CKM, with an estimated sensitivity of Typically less than 0.39 mU/ml. The ELISA analytical biochemical technique of the MBS908119 kit is based on CKM antibody-CKM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CKM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CKM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CKM elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CKM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CKM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CKM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CKM bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CKM elisa kit    CKM elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS908119. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CKM. It may not necessarily be applicable to this product.
 NCBI GI #    6671762
 NCBI GeneID    12715
 NCBI Accession #    NP_031736.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_007710.2 [Other Products]
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 UniProt Primary Accession #    P07310 [Other Products]
 UniProt Related Accession #    P07310 [Other Products]
 Molecular Weight    43,045 Da
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 NCBI Official Full Name    creatine kinase M-type
 NCBI Official Synonym Full Names    creatine kinase, muscle
 NCBI Official Symbol    Ckm [Similar Products]
 NCBI Official Synonym Symbols   
MCK; Ckmm; M-CK
[Similar Products]
 NCBI Protein Information    creatine kinase M-type; creatine kinase M chain
 UniProt Protein Name    Creatine kinase M-type
 UniProt Synonym Protein Names   
Creatine kinase M chain; M-CK
 Protein Family    Creatine kinase
 UniProt Gene Name    Ckm [Similar Products]
 UniProt Synonym Gene Names    Ckmm [Similar Products]
 UniProt Entry Name    KCRM_MOUSE
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 UniProt Comments for CKM    CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; Kinase, other; EC 2.7.3.2

Cellular Component: cytoplasm

Molecular Function: transferase activity; creatine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; catalytic activity; ATP binding

Biological Process: phosphocreatine metabolic process; phosphocreatine biosynthetic process; phosphorylation
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 Research Articles on CKM    1. PI3K and AKT regulated skeletal muscle differentiation by regulating the expression of Myogenin and MCK.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CKM elisa kitDiseases associated with CKM elisa kit
 Products by Pathway  Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Creatine Metabolism Pathway antibodies  Creatine Metabolism Pathway Diagram
 Creatine Pathway antibodies  Creatine Pathway Diagram
 Creatine Pathway antibodies  Creatine Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Urea Cycle And Metabolism Of Amino Groups Pathway antibodies  Urea Cycle And Metabolism Of Amino Groups Pathway Diagram
 Creatine Biosynthesis Pathway antibodies  Creatine Biosynthesis Pathway Diagram
 Creatine-phosphate Energy Transfer Pathway antibodies  Creatine-phosphate Energy Transfer Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >11 publications with CKM and Nervous System Diseases
 Heart Diseases Antibodies  >10 publications with CKM and Heart Diseases
 Neoplasms Antibodies  >10 publications with CKM and Neoplasms
 Myocardial Ischemia Antibodies  >9 publications with CKM and Myocardial Ischemia
 Myocardial Infarction Antibodies  >6 publications with CKM and Myocardial Infarction
 Neoplasm Metastasis Antibodies  >3 publications with CKM and Neoplasm Metastasis
 Kidney Diseases Antibodies  >3 publications with CKM and Kidney Diseases
 Lung Neoplasms Antibodies  >3 publications with CKM and Lung Neoplasms
 Renal Insufficiency Antibodies  >3 publications with CKM and Renal Insufficiency
 Drug Toxicity Antibodies  >2 publications with CKM and Drug Toxicity
Organs/Tissues associated with CKM elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >43 publications with CKM and Muscle
 Heart Antibodies  >22 publications with CKM and Heart
 Liver Antibodies  >6 publications with CKM and Liver
 Lung Antibodies  >5 publications with CKM and Lung
 Joint Antibodies  >3 publications with CKM and Joint
 Embryonic Tissue Antibodies  >3 publications with CKM and Embryonic Tissue
 Bone Antibodies  >2 publications with CKM and Bone
 Eye Antibodies  >2 publications with CKM and Eye
 Intestine Antibodies  >1 publications with CKM and Intestine
 Mammary Gland Antibodies  >1 publications with CKM and Mammary Gland
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