Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow CYP2E1 rightarrow Mouse CYP2E1  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • CUL3 Antibody
 • 2-amino-3-carboxymuconate-6 -semialdehyde decarboxylase (ACMS... ELISA Kit
 • REG3G Antibody
 • MYD88 (IN) Antibody
 • Fibrillin 1 (FBN1) ELISA Kit
 • Plasmin (PLG) Native Protein
 • Akt (Phospho-Ser124) Antibody
 • ACTB - Beta Actin Antibody
 • Exendin 4 ELISA Kit
 • 1-aminocyclopropane-1-carbo xylate synthase-like protein 1 (A... ELISA Kit
 • v-rel reticuloendotheliosis viral oncogene homolog A (avian)... ELISA Kit
 • p27 Kip1 (Phospho-Thr198) Antibody
 • ERBB2-HER2 Antibody
 • Soluble Intercellular Adhesion Molecule-1 (sICAM-1) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

CYP2E1 elisa kit :: Mouse cytochrome P450, family 2, subfamily E, polypeptide 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS933878
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Cytochrome P450 2E1, CYP2E1 ELISA Kit

 Product Synonym Names    Mouse Cytochrome P450 2E1 (CYP2E1) ELISA kit; CPE1; CYP2E; P450-J; P450C2E; cytochrome P450 2E1; cytochrome P450; subfamily IIE (ethanol-inducible); polypeptide 1; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenas; cytochrome P450; family 2; subfamily E; polypeptide 1
 Product Gene Name   

CYP2E1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS933878 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q05421
 Species Reactivity    Mouse
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse CYP2E1. No significant cross-reactivity or interference between mouse CYP2E1 and analogues was observed.
Section Bar
 Samples    Serum, plasma, tissue homogenates, cell lysates.
 Assay Type    Sandwich
 Detection Range    39 pg/ml-2500 pg/ml.
 Sensitivity    9.75 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CYP2E1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CYP2E1 purchase    MBS933878 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 39 pg/ml-2500 pg/ml in biological research samples containing CYP2E1, with an estimated sensitivity of 9.75 pg/ml. The ELISA analytical biochemical technique of the MBS933878 kit is based on CYP2E1 antibody-CYP2E1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP2E1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP2E1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for CYP2E1 elisa kit

   Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP2E1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP2E1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP2E1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP2E1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of CYP2E1 elisa kit    CYP2E1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS933878. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CYP2E1. It may not necessarily be applicable to this product.
 NCBI GI #    11276065
 NCBI GeneID    13106
 NCBI Accession #    NP_067257.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_021282.2 [Other Products]
Section Bar
 UniProt Primary Accession #    Q05421 [Other Products]
 UniProt Secondary Accession #    Q9Z198 [Other Products]
 UniProt Related Accession #    Q05421 [Other Products]
 Molecular Weight    56,805 Da
Table BarTOPTable Bar
 NCBI Official Full Name    cytochrome P450 2E1
 NCBI Official Synonym Full Names    cytochrome P450, family 2, subfamily e, polypeptide 1
 NCBI Official Symbol    Cyp2e1 [Similar Products]
 NCBI Official Synonym Symbols   
Cyp2e
[Similar Products]
 NCBI Protein Information    cytochrome P450 2E1; CYPIIE1; cytochrome P450-J; cytochrome P450-ALC; 4-nitrophenol 2-hydroxylase; cytochrome P450, 2e1, ethanol inducible
 UniProt Protein Name    Cytochrome P450 2E1
 UniProt Synonym Protein Names   
4-nitrophenol 2-hydroxylase (EC:1.14.13.n7); CYPIIE1; Cytochrome P450-ALC; Cytochrome P450-J
 Protein Family    Cytochrome
 UniProt Gene Name    Cyp2e1 [Similar Products]
 UniProt Synonym Gene Names    Cyp2e; Cyp2e-1 [Similar Products]
 UniProt Entry Name    CP2E1_MOUSE
Section Bar
 UniProt Comments for CYP2E1    CYP2E1: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.13.n7; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Lipid Metabolism - linoleic acid; Endoplasmic reticulum; Lipid Metabolism - arachidonic acid; Oxidoreductase; Mitochondrial

Cellular Component: Golgi membrane; intrinsic to endoplasmic reticulum membrane; membrane; intracellular membrane-bound organelle; mitochondrion; endoplasmic reticulum

Molecular Function: 3-(3-hydroxy-phenyl)propionate hydroxylase activity; toluene-sulfonate methyl-monooxygenase activity; xylene monooxygenase activity; chlorophenol 4-monooxygenase activity; 6-hydroxy-3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity; metal ion binding; oxidoreductase activity; carbon disulfide oxygenase activity; toluene 4-monooxygenase activity; phenanthrene 9,10-monooxygenase activity; 1-hydroxy-2-oxolimonene 1,2-monooxygenase activity; 2-hydroxy-phenylacetate hydroxylase activity; 4-chlorobenzaldehyde oxidase activity; enzyme binding; 2-oxo-delta3-4,5,5-trimethylcyclopentenylacetyl-CoA 1,2-monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; limonene monooxygenase activity; dibenzothiophene monooxygenase activity; phenanthrene 1,2-monooxygenase activity; 3,5-xylenol methylhydroxylase activity; 3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity; styrene monooxygenase activity; toluene 3-monooxygenase activity; monooxygenase activity; dimethyl sulfide monooxygenase activity; 4-hydroxyphenylacetate 3-monooxygenase activity; 4-nitrophenol 4-monooxygenase activity; 1-hydroxy-2-naphthoate hydroxylase activity; toluene-4-sulfonate monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; tetrahydrofuran hydroxylase activity; oxygen binding; phenylacetate hydroxylase activity; toluene 2-monooxygenase activity; iron ion binding; 6-hydroxynicotinate 3-monooxygenase activity; heme binding; phenanthrene 3,4-monooxygenase activity; arachidonic acid epoxygenase activity; alpha-pinene monooxygenase [NADH] activity; steroid hydroxylase activity; 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Biological Process: steroid metabolic process; triacylglycerol metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; monoterpenoid metabolic process; epoxygenase P450 pathway; heterocycle metabolic process; drug metabolic process
Table BarTOPTable Bar
 Research Articles on CYP2E1    1. significant increases in Col1a1, serine/threonine-protein kinase 1, Ctnnb1, CSRNP1, Ddit4, Cyp2e1, and Krit1 expressions and great decreases inreceptor D2, Neu1, and Dhcr7 expressions following long-term exposure to TiO2 NPs
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with CYP2E1 elisa kitDiseases associated with CYP2E1 elisa kit
 Products by Pathway  Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 CYP2E1 Reactions Pathway antibodies  CYP2E1 Reactions Pathway Diagram
 Chemical Carcinogenesis Pathway antibodies  Chemical Carcinogenesis Pathway Diagram
 Chemical Carcinogenesis Pathway antibodies  Chemical Carcinogenesis Pathway Diagram
 Cytochrome P450 Pathway antibodies  Cytochrome P450 Pathway Diagram
 Cytochrome P450 - Arranged By Substrate Type Pathway antibodies  Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
 Drug Metabolism - Cytochrome P450 Pathway antibodies  Drug Metabolism - Cytochrome P450 Pathway Diagram
 Drug Metabolism - Cytochrome P450 Pathway antibodies  Drug Metabolism - Cytochrome P450 Pathway Diagram
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >906 publications with CYP2E1 and Liver Diseases
 Drug-Induced Liver Injury Antibodies  >431 publications with CYP2E1 and Drug-Induced Liver Injury
 Necrosis Antibodies  >332 publications with CYP2E1 and Necrosis
 Fatty Liver Antibodies  >316 publications with CYP2E1 and Fatty Liver
 Poisoning Antibodies  >309 publications with CYP2E1 and Poisoning
 Drug-Related Side Effects and Adverse Reactions Antibodies  >263 publications with CYP2E1 and Drug-Related Side Effects and Adverse Reactions
 Liver Neoplasms Antibodies  >223 publications with CYP2E1 and Liver Neoplasms
 Alcoholism Antibodies  >211 publications with CYP2E1 and Alcoholism
 Inflammation Antibodies  >198 publications with CYP2E1 and Inflammation
 Hepatitis Antibodies  >167 publications with CYP2E1 and Hepatitis
Organs/Tissues associated with CYP2E1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >2760 publications with CYP2E1 and Liver
 Other Antibodies  >1024 publications with CYP2E1 and Other
 Lung Antibodies  >295 publications with CYP2E1 and Lung
 Kidney Antibodies  >223 publications with CYP2E1 and Kidney
 Muscle Antibodies  >96 publications with CYP2E1 and Muscle
 Intestine Antibodies  >72 publications with CYP2E1 and Intestine
 Stomach Antibodies  >56 publications with CYP2E1 and Stomach
 Skin Antibodies  >29 publications with CYP2E1 and Skin
 Embryonic Tissue Antibodies  >26 publications with CYP2E1 and Embryonic Tissue
 Prostate Antibodies  >20 publications with CYP2E1 and Prostate
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions