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CYP7A1 elisa kit :: Mouse cytochrome P450, family 7, subfamily A, polypeptide 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS922713
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1), ELISA Kit

 Also Known As   

Mouse Cholesterol 7-alpha-monooxygenase, CYP7A1 ELISA Kit

 Product Synonym Names    Mouse Cholesterol 7-alpha-monooxygenase (CYP7A1) ELISA kit; CP7A; CYP7; MGC126826; MGC138389; cholesterol 7 alpha-monooxygenase; cholesterol 7-alpha-hydroxylase; cytochrome P450; subfamily VIIA (cholesterol 7 alpha-monooxygenase); polypeptide 1; cytochrome P450; family 7; subfamily A; polypeptide 1
 Product Gene Name   

CYP7A1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS922713 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q64505
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse CYP7A1. No significant cross-reactivity or interference between mouse CYP7A1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    47 pg/ml -3000 pg/ml.
 Sensitivity    The minimum detectable dose of mouse CYP7A1 is typically less than 11.75 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CYP7A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CYP7A1 purchase    MBS922713 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 47 pg/ml -3000 pg/ml in biological research samples containing CYP7A1, with an estimated sensitivity of The minimum detectable dose of mouse CYP7A1 is typically less than 11.75 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS922713 kit is based on CYP7A1 antibody-CYP7A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP7A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP7A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CYP7A1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP7A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP7A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP7A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP7A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CYP7A1 elisa kit    CYP7A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS922713. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CYP7A1. It may not necessarily be applicable to this product.
 NCBI GI #    31542445
 NCBI GeneID    13122
 NCBI Accession #    NP_031850.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_007824.2 [Other Products]
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 UniProt Primary Accession #    Q64505 [Other Products]
 UniProt Secondary Accession #    Q8BFR7 [Other Products]
 UniProt Related Accession #    Q64505 [Other Products]
 Molecular Weight    57,262 Da
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 NCBI Official Full Name    cholesterol 7-alpha-monooxygenase
 NCBI Official Synonym Full Names    cytochrome P450, family 7, subfamily a, polypeptide 1
 NCBI Official Symbol    Cyp7a1 [Similar Products]
 NCBI Protein Information    cholesterol 7-alpha-monooxygenase; CYPVII; cytochrome P450 7A1; cytochrome P450, 7a1; cholesterol 7 alpha hydroxylase; cholesterol 7-alpha-hydroxylase
 UniProt Protein Name    Cholesterol 7-alpha-monooxygenase
 UniProt Synonym Protein Names   
CYPVII; Cholesterol 7-alpha-hydroxylase; Cytochrome P450 7A1
 Protein Family    Cholesterol 7-alpha-monooxygenase
 UniProt Gene Name    Cyp7a1 [Similar Products]
 UniProt Synonym Gene Names    Cyp7 [Similar Products]
 UniProt Entry Name    CP7A1_MOUSE
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 UniProt Comments for CYP7A1    CYP7A1: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - primary bile acid biosynthesis; Oxidoreductase; Endoplasmic reticulum; EC 1.14.13.17

Cellular Component: membrane; intracellular membrane-bound organelle; endoplasmic reticulum

Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; cholesterol 7-alpha-monooxygenase activity; metal ion binding; iron ion binding; heme binding; oxidoreductase activity; monooxygenase activity

Biological Process: steroid metabolic process; cholesterol metabolic process; bile acid biosynthetic process; cholesterol homeostasis; lipid metabolic process; cholesterol catabolic process
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 Research Articles on CYP7A1    1. Activation of the VDR represses hepatic SHP to increase levels of CYP7A1 and reduce cholesterol.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CYP7A1 elisa kitDiseases associated with CYP7A1 elisa kit
 Products by Pathway  Pathway Diagram
 Bile Acid And Bile Salt Metabolism Pathway antibodies  Bile Acid And Bile Salt Metabolism Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Cytochrome P450 - Arranged By Substrate Type Pathway antibodies  Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
 Endogenous Sterols Pathway antibodies  Endogenous Sterols Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Nuclear Receptors In Lipid Metabolism And Toxicity Pathway antibodies  Nuclear Receptors In Lipid Metabolism And Toxicity Pathway Diagram
 PPAR (Peroxisome Proliferator-activated Receptor) Signaling Pathway antibodies  PPAR (Peroxisome Proliferator-activated Receptor) Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Hypercholesterolemia Antibodies  >167 publications with CYP7A1 and Hypercholesterolemia
 Dyslipidemias Antibodies  >158 publications with CYP7A1 and Dyslipidemias
 Hyperlipidemias Antibodies  >152 publications with CYP7A1 and Hyperlipidemias
 Fatty Liver Antibodies  >149 publications with CYP7A1 and Fatty Liver
 Liver Diseases Antibodies  >135 publications with CYP7A1 and Liver Diseases
 Atherosclerosis Antibodies  >121 publications with CYP7A1 and Atherosclerosis
 Gallstones Antibodies  >105 publications with CYP7A1 and Gallstones
 Cardiovascular Diseases Antibodies  >75 publications with CYP7A1 and Cardiovascular Diseases
 Cholestasis Antibodies  >70 publications with CYP7A1 and Cholestasis
 Neoplasms Antibodies  >57 publications with CYP7A1 and Neoplasms
Organs/Tissues associated with CYP7A1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1256 publications with CYP7A1 and Liver
 Muscle Antibodies  >20 publications with CYP7A1 and Muscle
 Ovary Antibodies  >13 publications with CYP7A1 and Ovary
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