Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow ADH/VP/AVP rightarrow Mouse ADH/VP/AVP  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Collagen Type II (COL2A1) Antibody
 • Tumor Necrosis Factor alpha (TNFalpha) ELISA Kit
 • Acyl-Coenzyme A Dehydrogenase (ACADS) Recombinant Protein
 • beta NGF Recombinant Protein
 • Hyaluronic Acid (HA) ELISA Kit
 • glucagon like peptide 2 (GLP2) ELISA Kit
 • PECAM-1 Antibody
 • Vascular Endothelial Growth Factor (VEGF) Antibody
 • Low molecular weight protein-tyrosine-phosphatas e ptpA (ptpA... Recombinant Protein
 • Advanced Glycation End Product (AGE) Antibody
 • Nasoniavitripennis vitellogenin, Vtg ELISA Kit
 • ALDH1A1 Peptide
 • Macrophage Inflammatory protein-3 beta (MIP 3b) Recombinant Protein
 • Kappa Light Chain (LOC100037688) Secondary Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

ADH/VP/AVP elisa kit :: Mouse diuretic hormone/vasopressin/arginine vasopressin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS725028
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

diuretic hormone/vasopressin/arginine vasopressin (ADH/VP/AVP), ELISA Kit

★Popular Item★
 Also Known As   

Mouse antidiuretic hormone/vasopressin/arginine vasopressin ELISA Kit

 Product Synonym Names    Mouse antidiuretic hormone/vasopressin/arginine vasopressin ELISA Kit; antidiuretic hormone/vasopressin/arginine vasopressin; antidiuretic hormone/vasopressin/arginine vasopressin (Mouse)
 Product Gene Name   

ADH/VP/AVP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 20; NC_000020.10 (3063202..3065370, complement). Location: 20p13
 OMIM    125700
 3D Structure    ModBase 3D Structure for P01185
Section Bar
 Species Reactivity    Mouse
Section Bar
 Specificity    Sensitivity: The sensitivity in this assay is 1.0 pg/mL.

Specificity: This assay has high sensitivity and excellent specificity for detection of ADH. No significant cross-reactivity or interference between ADH and analogues was observed.
Section Bar
 Samples    Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADH/VP/AVP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADH/VP/AVP purchase    MBS725028 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the diuretic hormone/vasopressin/arginine vasopressin (ADH/VP/AVP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADH/VP/AVP. The ELISA analytical biochemical technique of the MBS725028 kit is based on ADH/VP/AVP antibody-ADH/VP/AVP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADH/VP/AVP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADH/VP/AVP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for ADH/VP/AVP elisa kit

   For Samples: Cell culture fluid & body fluid & tissue homogenate serum or blood plasma

Intended Uses: This ADH ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of ADH in the sample, this ADH ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus ADH concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of ADH utilizes a polyclonal anti-ADH antibody and an ADH-HRP conjugate. The assay sample and buffer are incubated together with ADH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADH concentration since ADH from samples and ADH-HRP conjugate compete for the anti-ADH antibody binding site. Since the number of sites is limited, as more sites are occupied by ADH from the sample, fewer sites are left to bind ADH-HRP conjugate. Standards of known ADH concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (O.D.) to the concentration of ADH. The ADH concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ADH/VP/AVP elisa kit    Mouse ELISA Kit
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of ADH/VP/AVP elisa kit    ADH/VP/AVP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS725028. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for ADH/VP/AVP. It may not necessarily be applicable to this product.
 NCBI GI #    116497129
 NCBI GeneID    551
 NCBI Accession #    AAI26197.1 [Other Products]
Section Bar
 UniProt Primary Accession #    P01185 [Other Products]
 UniProt Secondary Accession #    O14935; A0AV35 [Other Products]
 UniProt Related Accession #    P01185 [Other Products]
 Molecular Weight    17,325 Da
Table BarTOPTable Bar
 NCBI Official Full Name    Arginine vasopressin
 NCBI Official Synonym Full Names    arginine vasopressin
 NCBI Official Symbol    AVP [Similar Products]
 NCBI Official Synonym Symbols   
VP; ADH; ARVP; AVRP; AVP-NPII
[Similar Products]
 NCBI Protein Information    vasopressin-neurophysin 2-copeptin; neurohypophyseal; prepro-AVP-NP II; antidiuretic hormone; vasopressin-neurophysin II-copeptin; prepro-arginine-vasopressin-neurophysin II
 UniProt Protein Name    Vasopressin-neurophysin 2-copeptin
 UniProt Synonym Protein Names   
AVP-NPIICleaved into the following 3 chains:Arg-vasopressin; Alternative name(s):; Arginine-vasopressinNeurophysin 2; Alternative name(s):; Neurophysin-II
 UniProt Gene Name    AVP [Similar Products]
 UniProt Synonym Gene Names    ARVP; VP [Similar Products]
 UniProt Entry Name    NEU2_HUMAN
Section Bar
 NCBI Summary for ADH/VP/AVP    This gene encodes a precursor protein consisting of arginine vasopressin and two associated proteins, neurophysin 2 and a glycopeptide, copeptin. Arginine vasopressin is a posterior pituitary hormone which is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). [provided by RefSeq, Mar 2010]
Section Bar
 UniProt Comments for ADH/VP/AVP    Function: Neurophysin 2 specifically binds vasopressin.Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels.

Subcellular location: Secreted.

Involvement in disease: Diabetes insipidus, neurohypophyseal (NDI) [MIM:125700]: A disease characterized by persistent thirst, polydipsia and polyuria. Affected individuals are apparently normal at birth, but characteristically develop symptoms of vasopressin deficiency during childhood.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.4 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.27 Ref.28 Ref.29 Ref.30 Ref.31 Ref.32 Ref.33 Ref.34 Ref.35 Ref.36

Sequence similarities: Belongs to the vasopressin/oxytocin family.
Table BarTOPTable Bar
 Research Articles on ADH/VP/AVP    1. Data show that copeptin may indicate clinical severity of the initial bleeding and may therefore help in guiding treatment decisions in the setting of aneurysmal subarachnoid hemorrhage (aSAH).
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with ADH/VP/AVP elisa kitPathways associated with ADH/VP/AVP elisa kit
 Reference Product  PubMed Publications
 AVPR1B elisa kit  >2338 publications with ADH/VP/AVP and AVPR1B
 JUN elisa kit  >737 publications with ADH/VP/AVP and JUN
 CRH elisa kit  >513 publications with ADH/VP/AVP and CRH
 AQP2 elisa kit  >201 publications with ADH/VP/AVP and AQP2
 AVPR2 elisa kit  >41 publications with ADH/VP/AVP and AVPR2
 NOS3 elisa kit  >7 publications with ADH/VP/AVP and NOS3
 OXTR elisa kit  >7 publications with ADH/VP/AVP and OXTR
 Products by Pathway  Pathway Diagram
 Aquaporin-mediated Transport Pathway antibodies  Aquaporin-mediated Transport Pathway Diagram
 BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway antibodies  BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
Diseases associated with ADH/VP/AVP elisa kitOrgans/Tissues associated with ADH/VP/AVP elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >891 publications with ADH/VP/AVP and Cardiovascular Diseases
 Kidney Diseases Antibodies  >609 publications with ADH/VP/AVP and Kidney Diseases
 Hypertension Antibodies  >526 publications with ADH/VP/AVP and Hypertension
 Diabetes Insipidus Antibodies  >483 publications with ADH/VP/AVP and Diabetes Insipidus
 Brain Diseases Antibodies  >448 publications with ADH/VP/AVP and Brain Diseases
 Hyponatremia Antibodies  >316 publications with ADH/VP/AVP and Hyponatremia
 Dehydration Antibodies  >307 publications with ADH/VP/AVP and Dehydration
 Hypotension Antibodies  >256 publications with ADH/VP/AVP and Hypotension
 Heart Failure Antibodies  >243 publications with ADH/VP/AVP and Heart Failure
 Shock Antibodies  >197 publications with ADH/VP/AVP and Shock
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3239 publications with ADH/VP/AVP and Brain
 Kidney Antibodies  >1532 publications with ADH/VP/AVP and Kidney
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions