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DOC2B elisa kit :: Mouse double C2-like domains, beta ELISA Kit

Scan QR to view Datasheet Catalog #    MBS9326226 DOC2B elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
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 Product Name   

double C2-like domains, beta (DOC2B), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Double C2-like domain-containing protein beta, DOC2B ELISA Kit

 Product Synonym Names    Mouse Double C2-like domain-containing protein beta (DOC2B) ELISA kit; DOC2BL; double C2-like domains; beta-like; double C2-like domains; beta
 Product Gene Name   

DOC2B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P70169
 Species Reactivity    Mouse
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 Specificity    No significant cross-reactivity or interference between this analyte and analogues is observed.
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 Samples    Serum, Plasma and Tissue Homogenate
 Assay Type    Sandwich
 Detection Range    3.12 ng/ml - 100 ng/ml.
 Sensitivity    1.0 ng/ml.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100].
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DOC2B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DOC2B purchase    MBS9326226 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the double C2-like domains, beta (DOC2B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12 ng/ml - 100 ng/ml in biological research samples containing DOC2B, with an estimated sensitivity of 1.0 ng/ml. The ELISA analytical biochemical technique of the MBS9326226 kit is based on DOC2B antibody-DOC2B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DOC2B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DOC2B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma and Tissue Homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DOC2B elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of DOC2B (hereafter termed this analyte) in undiluted original Mouse serum, plasma and tissue homogenate samples.
Sample Manual Insert of MBS9326226. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DOC2B. It may not necessarily be applicable to this product.
 NCBI GI #    45433555
 NCBI GeneID    13447
 NCBI Accession #    NP_031899.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_007873.2 [Other Products]
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 UniProt Primary Accession #    P70169 [Other Products]
 UniProt Secondary Accession #    Q5SS41; Q6NXK3 [Other Products]
 UniProt Related Accession #    P70169 [Other Products]
 Molecular Weight    45,853 Da
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 NCBI Official Full Name    double C2-like domain-containing protein beta
 NCBI Official Synonym Full Names    double C2, beta
 NCBI Official Symbol    Doc2b [Similar Products]
 NCBI Protein Information    double C2-like domain-containing protein beta; doc2-beta; double C2 protein beta
 UniProt Protein Name    Double C2-like domain-containing protein beta
 Protein Family    Double C2-like domain-containing protein
 UniProt Gene Name    Doc2b [Similar Products]
 UniProt Synonym Gene Names    Doc2-beta [Similar Products]
 UniProt Entry Name    DOC2B_MOUSE
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 NCBI Summary for DOC2B    The protein encoded by this gene is a calcium sensor involved in glucose-stimulated insulin secretion, spontaneous neurotransmitter release, and enhanced SNARE-dependent vesicle fusion. [provided by RefSeq, Sep 2015]
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 UniProt Comments for DOC2B    DOC2B: Calcium sensor which positively regulates SNARE- dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes.

Protein type: Lipid-binding

Cellular Component: SNARE complex; membrane; cell; cytoplasm; plasma membrane

Molecular Function: protein binding; clathrin binding; syntaxin binding; calcium-dependent phospholipid binding; calcium ion binding

Biological Process: vesicle fusion; positive regulation of calcium ion-dependent exocytosis; protein localization; synaptic vesicle exocytosis; calcium ion-dependent exocytosis of neurotransmitter; positive regulation of insulin secretion; positive regulation of vesicle fusion; regulation of calcium ion-dependent exocytosis
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 Research Articles on DOC2B    1. Doc2b acts to inhibit vesicle priming during prolonged calcium elevations, thus protecting unprimed vesicles from fusing prematurely, and redirecting them to refill the readily releasable pool after relaxation of the calcium signal.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with DOC2B elisa kitOrgans/Tissues associated with DOC2B elisa kit
 Disease Name  Pubmed Publications
 Glucose Intolerance Antibodies  >1 publications with DOC2B and Glucose Intolerance
 Insulin Resistance Antibodies  >1 publications with DOC2B and Insulin Resistance
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >10 publications with DOC2B and Brain
 Embryonic Tissue Antibodies  >1 publications with DOC2B and Embryonic Tissue
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