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ELA2 elisa kit :: Mouse elastase 2, neutrophil ELISA Kit

Scan QR to view Datasheet Catalog #    MBS701009
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

elastase 2, neutrophil (ELA2), ELISA Kit

★Popular Item★
 Also Known As   

Mouse neutrophil elastase, NE ELISA Kit

 Product Synonym Names    Mouse neutrophil elastase; NE ELISA Kit; ELA2; GE; HLE; HNE; NE; PMN-E; SCN1; bone marrow serine protease; elastase 2; neutrophil; granulocyte-derived elastase; leukocyte elastase; medullasin; neutrophil elastase; polymorphonuclear elastase; elastase 2; neutrophil
 Product Gene Name   

ELA2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS701009 Testing Data    Testing Data PDF
 OMIM    202700
 3D Structure    ModBase 3D Structure for P08246
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse NE. No significant cross-reactivity or interference between mouse NE and analogues was observed.
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 Samples    serum, plasma, cell culture supernates, tissue homogenates
 Assay Type    Quantitative Sandwich
 Detection Range    0.312 ng/ml -20 ng/ml
 Sensitivity    <0.078 ng/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ELA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ELA2 purchase    MBS701009 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the elastase 2, neutrophil (ELA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml -20 ng/ml in biological research samples containing ELA2, with an estimated sensitivity of <0.078 ng/ml. The ELISA analytical biochemical technique of the MBS701009 kit is based on ELA2 antibody-ELA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ELA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ELA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ELA2 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NE bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ELA2 elisa kit    ELA2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS701009. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ELA2. It may not necessarily be applicable to this product.
 NCBI GI #    4503549
 NCBI GeneID    1991
 NCBI Accession #    NP_001963.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001972.2 [Other Products]
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 UniProt Primary Accession #    P08246 [Other Products]
 UniProt Secondary Accession #    P09649; Q6B0D9; Q6LDP5 [Other Products]
 UniProt Related Accession #    P08246 [Other Products]
 Molecular Weight    28,518 Da
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 NCBI Official Full Name    neutrophil elastase preproprotein
 NCBI Official Synonym Full Names    elastase, neutrophil expressed
 NCBI Official Symbol    ELANE [Similar Products]
 NCBI Official Synonym Symbols   
GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
[Similar Products]
 NCBI Protein Information    neutrophil elastase; elastase-2; medullasin; PMN elastase; leukocyte elastase; elastase 2, neutrophil; human leukocyte elastase; polymorphonuclear elastase; bone marrow serine protease; granulocyte-derived elastase
 UniProt Protein Name    Neutrophil elastase
 UniProt Synonym Protein Names   
Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase
 UniProt Gene Name    ELANE [Similar Products]
 UniProt Synonym Gene Names    ELA2; HLE [Similar Products]
 UniProt Entry Name    ELNE_HUMAN
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 NCBI Summary for ELA2    Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]
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 UniProt Comments for ELA2    Function: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.1 PublicationManual assertion based on experiment in:Ref.15

Catalytic activity: Hydrolysis of proteins, including elastin. Preferential cleavage: Val-|-Xaa > Ala-|-Xaa.
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 Research Articles on ELA2    1. Polymorphonuclear cell proteases, especially neutrophil elastase, degrade decorin, and this degradation renders collagen fibrils more susceptible to MMP-1 cleavage.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ELA2 elisa kitPathways associated with ELA2 elisa kit
 Reference Product  PubMed Publications
 MPO elisa kit  >2 publications with ELA2 and MPO
 Products by Pathway  Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Collagen Degradation Pathway antibodies  Collagen Degradation Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Systemic Lupus Erythematosus Pathway antibodies  Systemic Lupus Erythematosus Pathway Diagram
 Systemic Lupus Erythematosus Pathway antibodies  Systemic Lupus Erythematosus Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Amb2 Integrin Signaling Pathway antibodies  Amb2 Integrin Signaling Pathway Diagram
Diseases associated with ELA2 elisa kitOrgans/Tissues associated with ELA2 elisa kit
 Disease Name  Pubmed Publications
 Neutropenia Antibodies  >39 publications with ELA2 and Neutropenia
 Cyclic neutropenia Antibodies  >8 publications with ELA2 and Cyclic neutropenia
 Neutropenia, Severe Congenital, Autosomal Dominant 1 Antibodies  >5 publications with ELA2 and Neutropenia, Severe Congenital, Autosomal Dominant 1
 Lung Diseases Antibodies  >4 publications with ELA2 and Lung Diseases
 Disease Models, Animal Antibodies  >2 publications with ELA2 and Disease Models, Animal
 Weight Loss Antibodies  >2 publications with ELA2 and Weight Loss
 Inflammation Antibodies  >2 publications with ELA2 and Inflammation
 Shock Antibodies  >1 publications with ELA2 and Shock
 Liver Diseases Antibodies  >1 publications with ELA2 and Liver Diseases
 Pain Antibodies  >1 publications with ELA2 and Pain
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >12 publications with ELA2 and Blood
 Bone Marrow Antibodies  >4 publications with ELA2 and Bone Marrow
 Lung Antibodies  >3 publications with ELA2 and Lung
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