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EDN1 elisa kit :: Mouse endothelin 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703775
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $575 +1 FREE 8GB USB
96-Strip-Wells  /  $835 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

endothelin 1 (EDN1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Endothelin 1, ET-1 ELISA Kit

 Product Synonym Names    Mouse Endothelin 1; ET-1 ELISA Kit; ET1; HDLCQ7; preproendothelin 1; endothelin 1
 Product Gene Name   

EDN1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703775 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P22387
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse EDN1. No significant cross-reactivity or interference between Mouse EDN1 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates
 Detection Range    15.6 pg/ml-1000 pg/ml
 Sensitivity    3.9 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of EDN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for EDN1 purchase    MBS703775 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the endothelin 1 (EDN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml-1000 pg/ml in biological research samples containing EDN1, with an estimated sensitivity of 3.9 pg/ml. The ELISA analytical biochemical technique of the MBS703775 kit is based on EDN1 antibody-EDN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EDN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EDN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for EDN1 elisa kit

   Introduction: Endothelin-1(ET-1), a peptide of 21 amino acid residues, is the most potent vasoconstrictive substance known. Originally isolated from porcine aortic endothelial cells, ET-1 is now known to be one of a family of three mammalian vasoactive peptides that also includes Endothelin-2 (ET-2) and Endothelin-3 (ET-3). These related peptides differ from ET-1 at two and six amino acid residue positions, respectively. A fourth peptide, vasoactive intestinal contractor (VIC), is sometimes classified as rat ET-2. All members of the endothelin family contain two essential disulfide bridges and six conserved amino acid residues at the C-terminus. Additionally, all of the endothelin family members are synthesized initially as prepropolypeptides of approximately 200 amino acid residues encoded by separate genes. These are proteolytically cleaved to produce biologically-inactive propolypeptides of approximately 40 amino acid residues termed "big endothelins". Big ET-1 is cleaved by the proteolytic action of a membrane-bound metalloprotease [endothelin-converting enzyme (ECE-1)] producing the 21 amino acid residue active peptide. The biochemistry and biology of the endothelins have been the subject of several reviews.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ET-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ET-1. and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ET-1. in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of EDN1 elisa kit    EDN1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703775. Click to request current manual
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NCBI/Uniprot data below describe general gene information for EDN1. It may not necessarily be applicable to this product.
 NCBI GI #    6753720
 NCBI GeneID    13614
 NCBI Accession #    NP_034234.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_010104.3 [Other Products]
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 UniProt Primary Accession #    P22387 [Other Products]
 UniProt Secondary Accession #    P70203; P97338 [Other Products]
 UniProt Related Accession #    P22387 [Other Products]
 Molecular Weight    22,809 Da
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 NCBI Official Full Name    endothelin-1
 NCBI Official Synonym Full Names    endothelin 1
 NCBI Official Symbol    Edn1 [Similar Products]
 NCBI Official Synonym Symbols   
ET-1; preproET
[Similar Products]
 NCBI Protein Information    endothelin-1; PPET1; preproendothelin; preproendothelin-1
 UniProt Protein Name    Endothelin-1
 UniProt Synonym Protein Names   
Preproendothelin-1; PPET1
 Protein Family    Endothelin
 UniProt Gene Name    Edn1 [Similar Products]
 UniProt Synonym Gene Names    ET-1; PPET1 [Similar Products]
 UniProt Entry Name    EDN1_MOUSE
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 UniProt Comments for EDN1    Function: Endothelins are endothelium-derived vasoconstrictor peptides.
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 Research Articles on EDN1    1. Endothelin-1 drives development of glomerulosclerosis and podocyte loss through direct activation of endothelin receptors and NF-kappaB and beta-catenin pathways in podocytes
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with EDN1 elisa kitPathways associated with EDN1 elisa kit
 Reference Product  PubMed Publications
 EDNRA elisa kit  >32 publications with EDN1 and EDNRA
 EDNRB elisa kit  >22 publications with EDN1 and EDNRB
 ECE1 elisa kit  >12 publications with EDN1 and ECE1
 ADM elisa kit  >2 publications with EDN1 and ADM
 MME elisa kit  >1 publications with EDN1 and MME
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 MicroRNAs In Cardiomyocyte Hypertrophy Pathway antibodies  MicroRNAs In Cardiomyocyte Hypertrophy Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
Diseases associated with EDN1 elisa kitOrgans/Tissues associated with EDN1 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >34 publications with EDN1 and Cardiovascular Diseases
 Hypertension Antibodies  >30 publications with EDN1 and Hypertension
 Craniofacial Abnormalities Antibodies  >19 publications with EDN1 and Craniofacial Abnormalities
 Inflammation Antibodies  >19 publications with EDN1 and Inflammation
 Asthma Antibodies  >13 publications with EDN1 and Asthma
 Kidney Diseases Antibodies  >8 publications with EDN1 and Kidney Diseases
 Cardiovascular Abnormalities Antibodies  >7 publications with EDN1 and Cardiovascular Abnormalities
 Heart Failure Antibodies  >5 publications with EDN1 and Heart Failure
 Heart Defects, Congenital Antibodies  >5 publications with EDN1 and Heart Defects, Congenital
 Myocardial Ischemia Antibodies  >5 publications with EDN1 and Myocardial Ischemia
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >31 publications with EDN1 and Vascular
 Brain Antibodies  >10 publications with EDN1 and Brain
 Skin Antibodies  >7 publications with EDN1 and Skin
 Liver Antibodies  >2 publications with EDN1 and Liver
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