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EPX elisa kit :: Mouse eosinophil peroxidase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705531
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

eosinophil peroxidase (EPX), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Eosinophil Peroxidase, EPO ELISA Kit

 Product Synonym Names    Mouse Eosinophil Peroxidase (EPO) ELISA Kit; EPO; EPP; EPX-PEN; ; eosinophil peroxidase
 Product Gene Name   

EPX elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705531 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P49290
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse EPO. No significant cross-reactivity or interference between mouse EPO and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates.
 Assay Type    Sandwich (Quantitative)
 Detection Range    0.156 ng/ml-10 ng/ml.
 Sensitivity    0.039 ng/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of EPX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for EPX purchase    MBS705531 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the eosinophil peroxidase (EPX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 ng/ml-10 ng/ml in biological research samples containing EPX, with an estimated sensitivity of 0.039 ng/ml. The ELISA analytical biochemical technique of the MBS705531 kit is based on EPX antibody-EPX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EPX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EPX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for EPX elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPO bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of EPX elisa kit    EPX elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705531. Click to request current manual
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NCBI/Uniprot data below describe general gene information for EPX. It may not necessarily be applicable to this product.
 NCBI GI #    145966840
 NCBI GeneID    13861
 NCBI Accession #    NP_031972.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_007946.2 [Other Products]
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 UniProt Primary Accession #    P49290 [Other Products]
 UniProt Secondary Accession #    Q5SW51; Q61798 [Other Products]
 UniProt Related Accession #    P49290 [Other Products]
 Molecular Weight    81,380 Da
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 NCBI Official Full Name    eosinophil peroxidase
 NCBI Official Synonym Full Names    eosinophil peroxidase
 NCBI Official Symbol    Epx [Similar Products]
 NCBI Official Synonym Symbols   
EPO
[Similar Products]
 NCBI Protein Information    eosinophil peroxidase
 UniProt Protein Name    Eosinophil peroxidase
 Protein Family    Eosinophil peroxidase
 UniProt Gene Name    Epx [Similar Products]
 UniProt Synonym Gene Names    Eper; EPO [Similar Products]
 UniProt Entry Name    PERE_MOUSE
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 UniProt Comments for EPX    Function: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils 1 PublicationManual assertion based on experiment in:Ref.4

Catalytic activity: 2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactor: Binds 1 calcium ion per heterodimer
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 Research Articles on EPX    1. The absence of MBP-1 and EPX promoted a concomitant loss of eosinophil lineage-committed progenitors in the marrow, identifying a specific blockade in eosinophilopoiesis as the causative event.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with EPX elisa kitPathways associated with EPX elisa kit
 Reference Product  PubMed Publications
 EPOR elisa kit  >617 publications with EPX and EPOR
 HIF1A elisa kit  >213 publications with EPX and HIF1A
 RB1 elisa kit  >1 publications with EPX and RB1
 Products by Pathway  Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Phosphate Acquisition II Pathway antibodies  Phosphate Acquisition II Pathway Diagram
Diseases associated with EPX elisa kitOrgans/Tissues associated with EPX elisa kit
 Disease Name  Pubmed Publications
 Respiratory Tract Diseases Antibodies  >90 publications with EPX and Respiratory Tract Diseases
 Asthma Antibodies  >89 publications with EPX and Asthma
 Inflammation Antibodies  >85 publications with EPX and Inflammation
 Lung Diseases Antibodies  >77 publications with EPX and Lung Diseases
 Skin Diseases Antibodies  >24 publications with EPX and Skin Diseases
 Eosinophilia Antibodies  >23 publications with EPX and Eosinophilia
 Respiratory Sounds Antibodies  >8 publications with EPX and Respiratory Sounds
 Bronchial Hyperreactivity Antibodies  >8 publications with EPX and Bronchial Hyperreactivity
 Fibrosis Antibodies  >7 publications with EPX and Fibrosis
 Necrosis Antibodies  >5 publications with EPX and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >638 publications with EPX and Brain
 Eye Antibodies  >118 publications with EPX and Eye
 Prostate Antibodies  >27 publications with EPX and Prostate
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