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| Product Name
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eosinophil peroxidase (EPX), ELISA Kit ★Popular Item★
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| Also Known As
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Mouse Eosinophil Peroxidase, EPO ELISA Kit
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| Product Synonym Names
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Mouse Eosinophil Peroxidase (EPO) ELISA Kit; EPO; EPP; EPX-PEN; ; eosinophil peroxidase
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| MBS705531 Testing Data
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| 3D Structure
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ModBase 3D Structure for P49290
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| Species Reactivity
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Mouse
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| Specificity
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This assay has high sensitivity and excellent specificity for detection of mouse EPO. No significant cross-reactivity or interference between mouse EPO and analogues was observed.
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| Samples
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Serum, plasma, tissue homogenates.
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| Assay Type
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Sandwich (Quantitative)
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| Detection Range
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0.156 ng/ml-10 ng/ml.
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| Sensitivity
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0.039 ng/ml.
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| Intra-assay Precision
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Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
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| Inter-assay Precision
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Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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| Preparation and Storage
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of EPX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for EPX purchase
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MBS705531 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the eosinophil peroxidase (EPX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 ng/ml-10 ng/ml in biological research samples containing EPX, with an estimated sensitivity of 0.039 ng/ml. The ELISA analytical biochemical technique of the MBS705531 kit is based on EPX antibody-EPX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EPX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EPX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for EPX elisa kit
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPO bound in the initial step. The color development is stopped and the intensity of the color is measured.
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| Typical Testing Data/Standard Curve (for reference only) of EPX elisa kit
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| Sample Manual Insert of MBS705531. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for EPX. It may not necessarily be applicable to this product. |
| NCBI GI #
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145966840
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| NCBI GeneID
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13861
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| NCBI Accession #
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NP_031972.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_007946.2
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[Other Products]
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| UniProt Primary Accession #
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P49290
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[Other Products]
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| UniProt Secondary Accession #
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Q5SW51; Q61798
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[Other Products]
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| UniProt Related Accession #
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P49290
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[Other Products]
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| Molecular Weight
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81,380 Da
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| NCBI Official Full Name
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eosinophil peroxidase
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| NCBI Official Synonym Full Names
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eosinophil peroxidase
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| NCBI Official Symbol
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Epx
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[Similar Products]
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| NCBI Official Synonym Symbols
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EPO
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[Similar Products]
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| NCBI Protein Information
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eosinophil peroxidase
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| UniProt Protein Name
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Eosinophil peroxidase
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| Protein Family
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Eosinophil peroxidase
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| UniProt Gene Name
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Epx
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[Similar Products]
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| UniProt Synonym Gene Names
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Eper; EPO
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[Similar Products]
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| UniProt Entry Name
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PERE_MOUSE
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| UniProt Comments for EPX
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Function: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils 1 PublicationManual assertion based on experiment in:Ref.4
Catalytic activity: 2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.
Cofactor: Binds 1 calcium ion per heterodimer
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| Research Articles on EPX
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1. The absence of MBP-1 and EPX promoted a concomitant loss of eosinophil lineage-committed progenitors in the marrow, identifying a specific blockade in eosinophilopoiesis as the causative event.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with EPX elisa kit | Pathways associated with EPX elisa kit |
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| Diseases associated with EPX elisa kit | Organs/Tissues associated with EPX elisa kit |
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ELISA Kit 
