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EIF2C2 elisa kit :: Mouse eukaryotic translation initiation factor 2C, 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS911752
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

eukaryotic translation initiation factor 2C, 2 (EIF2C2), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Protein argonaute-2, EIF2C2 ELISA Kit

 Product Synonym Names    Mouse Protein argonaute-2 (EIF2C2) ELISA kit; AGO2; MGC3183; Q10; argonaute 2; eukaryotic translation initiation factor 2C; 2
 Product Gene Name   

EIF2C2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS911752 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q8CJG0
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse EIF2C2. No significant cross-reactivity or interference between mouse EIF2C2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml.
 Sensitivity    The minimum detectable dose of mouse EIF2C2 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of EIF2C2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for EIF2C2 purchase    MBS911752 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the eukaryotic translation initiation factor 2C, 2 (EIF2C2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing EIF2C2, with an estimated sensitivity of The minimum detectable dose of mouse EIF2C2 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS911752 kit is based on EIF2C2 antibody-EIF2C2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EIF2C2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EIF2C2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for EIF2C2 elisa kit

  
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EIF2C2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EIF2C2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EIF2C2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EIF2C2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of EIF2C2 elisa kit    EIF2C2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS911752. Click to request current manual
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NCBI/Uniprot data below describe general gene information for EIF2C2. It may not necessarily be applicable to this product.
 NCBI GI #    219842353
 NCBI GeneID    239528
 NCBI Accession #    NP_694818.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_153178.4 [Other Products]
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 UniProt Primary Accession #    Q8CJG0 [Other Products]
 UniProt Secondary Accession #    Q4VAB3; Q571J6; A1A563 [Other Products]
 UniProt Related Accession #    Q8CJG0 [Other Products]
 Molecular Weight    97,304 Da
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 NCBI Official Full Name    protein argonaute-2
 NCBI Official Synonym Full Names    argonaute RISC catalytic subunit 2
 NCBI Official Symbol    Ago2 [Similar Products]
 NCBI Official Synonym Symbols   
Eif2c2; Gerp95; Gm10365; AI225898; AL022874; AW546247; mKIAA4215; 1110029L17Rik; 2310051F07Rik; ENSMUSG00000072493
[Similar Products]
 NCBI Protein Information    protein argonaute-2
 UniProt Protein Name    Protein argonaute-2
 UniProt Synonym Protein Names   
Argonaute RISC catalytic component 2; Eukaryotic translation initiation factor 2C 2; eIF-2C 2; eIF2C 2; Piwi/argonaute family protein meIF2C2; Protein slicer
 UniProt Gene Name    Ago2 [Similar Products]
 UniProt Synonym Gene Names    Eif2c2; Kiaa4215; Argonaute2; mAgo2; eIF-2C 2; eIF2C 2 [Similar Products]
 UniProt Entry Name    AGO2_MOUSE
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 UniProt Comments for EIF2C2    AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Protein type: RNA processing; RNA-binding; EC 3.1.26.n2

Cellular Component: polysome; mRNA cap complex; membrane; cytoplasm; dendrite; nucleus; cytosol; ribonucleoprotein complex

Molecular Function: mRNA binding; endoribonuclease activity; protein binding; siRNA binding; nuclease activity; miRNA binding; nucleic acid binding; hydrolase activity; RNA binding; endonuclease activity; metal ion binding; RNA 7-methylguanosine cap binding

Biological Process: regulation of translation; negative regulation of translational initiation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; pre-microRNA processing; multicellular organismal development; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, negative regulation of translation; RNA-mediated gene silencing; cell differentiation; post-embryonic development; mRNA cleavage
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 Research Articles on EIF2C2    1. Increased AGO2 was detected in autophagy-deficient ATG5-/- and ATG16-/- mouse embryonic fibroblast cells. Chemical agents and VacA toxin, which disrupt autophagy, increased AGO2 expression in MEFs, epithelial cells lines, and human monocytes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with EIF2C2 elisa kitDiseases associated with EIF2C2 elisa kit
 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 MicroRNA (miRNA) Biogenesis Pathway antibodies  MicroRNA (miRNA) Biogenesis Pathway Diagram
 Post-transcriptional Silencing By Small RNAs Pathway antibodies  Post-transcriptional Silencing By Small RNAs Pathway Diagram
 Regulatory RNA Pathways antibodies  Regulatory RNA Pathways Diagram
 Small Interfering RNA (siRNA) Biogenesis Pathway antibodies  Small Interfering RNA (siRNA) Biogenesis Pathway Diagram
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >6 publications with EIF2C2 and Breast Neoplasms
 Adenocarcinoma Antibodies  >6 publications with EIF2C2 and Adenocarcinoma
 Necrosis Antibodies  >6 publications with EIF2C2 and Necrosis
 Nervous System Diseases Antibodies  >4 publications with EIF2C2 and Nervous System Diseases
 Lung Diseases Antibodies  >3 publications with EIF2C2 and Lung Diseases
 Atrophy Antibodies  >3 publications with EIF2C2 and Atrophy
 Neoplasms, Experimental Antibodies  >3 publications with EIF2C2 and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >2 publications with EIF2C2 and Carcinoma, Hepatocellular
 Vascular Diseases Antibodies  >2 publications with EIF2C2 and Vascular Diseases
 Inflammation Antibodies  >2 publications with EIF2C2 and Inflammation
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