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BF elisa kit :: Mouse factor B, BF ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700168
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $575 +1 FREE 8GB USB
96-Strip-Wells  /  $835 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
 
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 Product Name   

factor B, BF, ELISA Kit

 Also Known As   

Mouse factor B, BF ELISA Kit

 Product Synonym Names    Mouse factor B; BF ELISA Kit; factor B; BF
 Product Gene Name   

BF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700168 Testing Data    Testing Data PDF
 OMIM    615561
 3D Structure    ModBase 3D Structure for P00751
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse BF. No significant cross-reactivity or interference between Mouse BF and analogues was observed.
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 Samples    Serum, plasma
 Detection Range    0.78 ng/ml-50 ng/ml
 Sensitivity    0.195 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BF purchase    MBS700168 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the factor B, BF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.78 ng/ml-50 ng/ml in biological research samples containing BF, with an estimated sensitivity of 0.195 ng/ml. The ELISA analytical biochemical technique of the MBS700168 kit is based on BF antibody-BF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for BF elisa kit

   Introduction: Complement factor B is a protein that in humans is encoded by the CFB gene. This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease that associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for BF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain BF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of BF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of BF elisa kit    BF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700168. Click to request current manual
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NCBI/Uniprot data below describe general gene information for BF. It may not necessarily be applicable to this product.
 NCBI GI #    13560705
 NCBI GeneID    629
 NCBI Accession #    AAK30167.1 [Other Products]
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 UniProt Primary Accession #    P00751 [Other Products]
 UniProt Secondary Accession #    O15006; Q29944; Q53F89; Q5JP67; Q5ST50; Q96HX6; Q9BTF5; Q9BX92; B0QZQ6 [Other Products]
 UniProt Related Accession #    P00751 [Other Products]
 Molecular Weight    68,872 Da
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 NCBI Official Full Name    factor B
 NCBI Official Synonym Full Names    complement factor B
 NCBI Official Symbol    CFB [Similar Products]
 NCBI Official Synonym Symbols   
BF; FB; BFD; GBG; CFAB; CFBD; PBF2; AHUS4; FBI12; H2-Bf; ARMD14
[Similar Products]
 NCBI Protein Information    complement factor B; C3 proactivator; C3/C5 convertase; C3 proaccelerator; properdin factor B; B-factor, properdin; glycine-rich beta glycoprotein; glycine-rich beta-glycoprotein
 UniProt Protein Name    Complement factor B
 UniProt Synonym Protein Names   
C3/C5 convertase; Glycine-rich beta glycoprotein; GBG; PBF2; Properdin factor BCleaved into the following 2 chains:Complement factor B Ba fragment; Complement factor B Bb fragment
 UniProt Gene Name    CFB [Similar Products]
 UniProt Synonym Gene Names    BF; BFD; GBG [Similar Products]
 UniProt Entry Name    CFAB_HUMAN
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 NCBI Summary for BF    This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq, Jul 2008]
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 UniProt Comments for BF    Function: Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.

Catalytic activity: Cleavage of Arg-|-Ser bond in complement component C3 alpha-chain to yield C3a and C3b, and Arg-|-Xaa bond in complement component C5 alpha-chain to yield C5a and C5b.
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 Research Articles on BF    1. The CFB (R32Q) polymorphism was associated with age-related macular degeneration characterized by small drusen only, and appeared to be protective of large drusen.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with BF elisa kitPathways associated with BF elisa kit
 Reference Product  PubMed Publications
 CFI elisa kit  >29 publications with BF and CFI
 SERPING1 elisa kit  >6 publications with BF and SERPING1
 C1R elisa kit  >3 publications with BF and C1R
 CFP elisa kit  >3 publications with BF and CFP
 C4A elisa kit  >1 publications with BF and C4A
 Products by Pathway  Pathway Diagram
 Activation Of C3 And C5 Pathway antibodies  Activation Of C3 And C5 Pathway Diagram
 Alternative Complement Activation Pathway antibodies  Alternative Complement Activation Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Initial Triggering Of Complement Pathway antibodies  Initial Triggering Of Complement Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Regulation Of Complement Cascade Pathway antibodies  Regulation Of Complement Cascade Pathway Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram
Diseases associated with BF elisa kitOrgans/Tissues associated with BF elisa kit
 Disease Name  Pubmed Publications
 Macular Degeneration Antibodies  >78 publications with BF and Macular Degeneration
 Cardiovascular Diseases Antibodies  >23 publications with BF and Cardiovascular Diseases
 Inflammation Antibodies  >21 publications with BF and Inflammation
 Immune System Diseases Antibodies  >19 publications with BF and Immune System Diseases
 Nervous System Diseases Antibodies  >15 publications with BF and Nervous System Diseases
 Necrosis Antibodies  >14 publications with BF and Necrosis
 Fibrosis Antibodies  >14 publications with BF and Fibrosis
 Kidney Diseases Antibodies  >14 publications with BF and Kidney Diseases
 Atypical hemolytic uremic syndrome Antibodies  >13 publications with BF and Atypical hemolytic uremic syndrome
 Heart Diseases Antibodies  >11 publications with BF and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >57 publications with BF and Blood
 Eye Antibodies  >42 publications with BF and Eye
 Heart Antibodies  >25 publications with BF and Heart
 Muscle Antibodies  >21 publications with BF and Muscle
 Liver Antibodies  >20 publications with BF and Liver
 Intestine Antibodies  >12 publications with BF and Intestine
 Kidney Antibodies  >9 publications with BF and Kidney
 Bone Antibodies  >8 publications with BF and Bone
 Mouth Antibodies  >7 publications with BF and Mouth
 Brain Antibodies  >6 publications with BF and Brain
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