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FABP4 elisa kit :: Mouse fatty acid binding protein 4, adipocyte ELISA Kit

Scan QR to view Datasheet Catalog #    MBS925921 FABP4 elisa kit
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

fatty acid binding protein 4, adipocyte (FABP4), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Fatty acid-binding protein, adipocyte, FABP4 ELISA Kit

 Product Synonym Names    Mouse Fatty acid-binding protein; adipocyte (FABP4) ELISA kit; A-FABP; aP2; ; fatty acid binding protein 4; adipocyte
 Product Gene Name   

FABP4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P04117
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse FABP4. No significant cross-reactivity or interference between mouse FABP4 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Quantitative Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml
 Sensitivity    7.81 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FABP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FABP4 purchase    MBS925921 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the fatty acid binding protein 4, adipocyte (FABP4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing FABP4, with an estimated sensitivity of 7.81 pg/ml. The ELISA analytical biochemical technique of the MBS925921 kit is based on FABP4 antibody-FABP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FABP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FABP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FABP4 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FABP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FABP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FABP4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FABP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS925921. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FABP4. It may not necessarily be applicable to this product.
 NCBI GI #    14149635
 NCBI GeneID    11770
 NCBI Accession #    NP_077717.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_024406.2 [Other Products]
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 UniProt Primary Accession #    P04117 [Other Products]
 UniProt Related Accession #    P04117 [Other Products]
 Molecular Weight    14,650 Da
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 NCBI Official Full Name    fatty acid-binding protein, adipocyte
 NCBI Official Synonym Full Names    fatty acid binding protein 4, adipocyte
 NCBI Official Symbol    Fabp4 [Similar Products]
 NCBI Official Synonym Symbols   
Ap2; Lbpl; 422/aP2; ALBP/Ap2
[Similar Products]
 NCBI Protein Information    fatty acid-binding protein, adipocyte; P15; ALBP; AFABP; A-FABP; protein 422; P2 adipocyte protein; adipocyte protein aP2; myelin P2 protein homolog; 3T3-L1 lipid-binding protein; fatty acid-binding protein 4; adipocyte lipid-binding protein; adipocyte-type fatty acid-binding protein
 UniProt Protein Name    Fatty acid-binding protein, adipocyte
 UniProt Synonym Protein Names   
3T3-L1 lipid-binding protein; Adipocyte lipid-binding protein; ALBP; Adipocyte-type fatty acid-binding protein; A-FABP; AFABP; Fatty acid-binding protein 4; Myelin P2 protein homolog; P15; P2 adipocyte protein; Protein 422
 Protein Family    Fatty acid-binding protein
 UniProt Gene Name    Fabp4 [Similar Products]
 UniProt Synonym Gene Names    Ap2; ALBP; A-FABP; AFABP [Similar Products]
 UniProt Entry Name    FABP4_MOUSE
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 UniProt Comments for FABP4    FABP4: a lipid transport protein that is the predominant fatty acid binding protein found in adipocytes. Also expressed in macrophages. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Homodimer. Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Interacts with PPARG. Monomer. FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes. Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE. Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders. Belongs to the calycin superfamily,fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: transporter activity; lipid binding

Biological Process: cholesterol homeostasis; transport; positive regulation of cell proliferation; white fat cell differentiation; brown fat cell differentiation; cytokine production; negative regulation of protein kinase activity; fatty acid metabolic process; negative regulation of transcription, DNA-dependent; positive regulation of inflammatory response
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 Research Articles on FABP4    1. found that the FABP4 level was higher and PPARgamma level was lower in human visceral fat and mouse epididymal fat compared with their subcutaneous fat
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FABP4 elisa kitPathways associated with FABP4 elisa kit
 Reference Product  PubMed Publications
 Pparg elisa kit  >103 publications with FABP4 and Pparg
 Cebpa elisa kit  >26 publications with FABP4 and Cebpa
 Cebpb elisa kit  >4 publications with FABP4 and Cebpb
 Sirt1 elisa kit  >1 publications with FABP4 and Sirt1
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway antibodies  Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 PPAR (Peroxisome Proliferator-activated Receptor) Signaling Pathway antibodies  PPAR (Peroxisome Proliferator-activated Receptor) Signaling Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies  Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram
Diseases associated with FABP4 elisa kitOrgans/Tissues associated with FABP4 elisa kit
 Disease Name  Pubmed Publications
 Obesity Antibodies  >108 publications with FABP4 and Obesity
 Insulin Resistance Antibodies  >80 publications with FABP4 and Insulin Resistance
 Inflammation Antibodies  >51 publications with FABP4 and Inflammation
 Hyperinsulinism Antibodies  >48 publications with FABP4 and Hyperinsulinism
 Atherosclerosis Antibodies  >47 publications with FABP4 and Atherosclerosis
 Cardiovascular Diseases Antibodies  >40 publications with FABP4 and Cardiovascular Diseases
 Diabetes Mellitus Antibodies  >35 publications with FABP4 and Diabetes Mellitus
 Fatty Liver Antibodies  >32 publications with FABP4 and Fatty Liver
 Necrosis Antibodies  >20 publications with FABP4 and Necrosis
 Neoplasms Antibodies  >18 publications with FABP4 and Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Adipose Tissue Antibodies  >134 publications with FABP4 and Adipose Tissue
 Connective Tissue Antibodies  >88 publications with FABP4 and Connective Tissue
 Muscle Antibodies  >53 publications with FABP4 and Muscle
 Liver Antibodies  >39 publications with FABP4 and Liver
 Bone Marrow Antibodies  >25 publications with FABP4 and Bone Marrow
 Heart Antibodies  >19 publications with FABP4 and Heart
 Embryonic Tissue Antibodies  >11 publications with FABP4 and Embryonic Tissue
 Kidney Antibodies  >8 publications with FABP4 and Kidney
 Lung Antibodies  >8 publications with FABP4 and Lung
 Eye Antibodies  >6 publications with FABP4 and Eye
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