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FNDC5 elisa kit :: Mouse fibronectin type III domain containing 5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS901062
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

fibronectin type III domain containing 5 (FNDC5), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Fibronectin type III domain-containing protein 5, FNDC5 ELISA Kit

 Product Synonym Names    Mouse Fibronectin type III domain-containing protein 5 (FNDC5) ELISA kit; FRCP2; ; fibronectin type III domain containing 5
 Product Gene Name   

FNDC5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS901062 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q8K4Z2
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse FNDC5. No significant cross-reactivity or interference between mouse FNDC5 and analogues was observed.
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 Samples    Serum, plasma and tissue homogenates
 Assay Type    Quantitative Sandwich
 Detection Range    6.25 pg/ml -400 pg/ml
 Sensitivity    Typically less than 1.56 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FNDC5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FNDC5 purchase    MBS901062 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the fibronectin type III domain containing 5 (FNDC5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 pg/ml -400 pg/ml in biological research samples containing FNDC5, with an estimated sensitivity of Typically less than 1.56 pg/ml. The ELISA analytical biochemical technique of the MBS901062 kit is based on FNDC5 antibody-FNDC5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FNDC5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FNDC5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FNDC5 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FNDC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FNDC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FNDC5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FNDC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of FNDC5 elisa kit    FNDC5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS901062. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FNDC5. It may not necessarily be applicable to this product.
 NCBI GI #    47059145
 NCBI GeneID    384061
 NCBI Accession #    NP_081678.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_027402.3 [Other Products]
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 UniProt Primary Accession #    Q8K4Z2 [Other Products]
 UniProt Secondary Accession #    Q9DB97 [Other Products]
 UniProt Related Accession #    Q8K4Z2 [Other Products]
 Molecular Weight    23,321 Da
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 NCBI Official Full Name    fibronectin type III domain-containing protein 5
 NCBI Official Synonym Full Names    fibronectin type III domain containing 5
 NCBI Official Symbol    Fndc5 [Similar Products]
 NCBI Official Synonym Symbols   
PeP; Pxp; C87088; AI836596; 1500001L03Rik
[Similar Products]
 NCBI Protein Information    fibronectin type III domain-containing protein 5; peroxisomal protein; fibronectin type III repeat-containing protein 2
 UniProt Protein Name    Fibronectin type III domain-containing protein 5
 UniProt Synonym Protein Names   
Fibronectin type III repeat-containing protein 2; Peroxisomal protein; PeP
 Protein Family    Fibronectin type III domain-containing protein
 UniProt Gene Name    Fndc5 [Similar Products]
 UniProt Synonym Gene Names    Frcp2; Pep; PeP [Similar Products]
 UniProt Entry Name    FNDC5_MOUSE
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 UniProt Comments for FNDC5    FNDC5: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; endoplasmic reticulum; extracellular region; integral to membrane; plasma membrane; peroxisome

Molecular Function: protein binding; hormone activity

Biological Process: response to muscle activity
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 Research Articles on FNDC5    1. These findings link endurance exercise and the important metabolic mediators, PGC-1alpha and FNDC5, with BDNF expression in the brain.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with FNDC5 elisa kitOrgans/Tissues associated with FNDC5 elisa kit
 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >6 publications with FNDC5 and Fatty Liver
 Weight Loss Antibodies  >6 publications with FNDC5 and Weight Loss
 Liver Diseases Antibodies  >4 publications with FNDC5 and Liver Diseases
 Inflammation Antibodies  >4 publications with FNDC5 and Inflammation
 Necrosis Antibodies  >3 publications with FNDC5 and Necrosis
 Rhabdomyosarcoma Antibodies  >2 publications with FNDC5 and Rhabdomyosarcoma
 Colonic Neoplasms Antibodies  >1 publications with FNDC5 and Colonic Neoplasms
 Disease Models, Animal Antibodies  >1 publications with FNDC5 and Disease Models, Animal
 Hyperplasia Antibodies  >1 publications with FNDC5 and Hyperplasia
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >3 publications with FNDC5 and Muscle
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