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GGT5 elisa kit :: Mouse gamma-glutamyltransferase 5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS940420 GGT5 elisa kit
Unit / Price
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,550 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,815 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

gamma-glutamyltransferase 5 (GGT5), ELISA Kit

 Also Known As   

Mouse Gamma-glutamyltransferase 5, GGT5 ELISA Kit

 Product Synonym Names    Mouse Gamma-glutamyltransferase 5 (GGT5) ELISA kit; DKFZp566O011; FLJ92733; GGT-REL; GGTLA1; gamma-glutamyl cleaving enzyme; gamma-glutamyl transpeptidase-related protein; gamma-glutamyltransferase-like activity 1; gamma-glutamyltranspeptidase 5; gamma-glutamyltransferase 5
 Product Gene Name   

GGT5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 3D Structure    ModBase 3D Structure for Q9Z2A9
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse GGT5. No significant cross-reactivity or interference between mouse GGT5 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    12.5 pg/ml-800 pg/ml.
 Sensitivity    The minimum detectable dose of mouse GGT5 is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of GGT5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GGT5 purchase    MBS940420 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the gamma-glutamyltransferase 5 (GGT5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 12.5 pg/ml-800 pg/ml in biological research samples containing GGT5, with an estimated sensitivity of The minimum detectable dose of mouse GGT5 is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS940420 kit is based on GGT5 antibody-GGT5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GGT5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GGT5. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for GGT5 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GGT5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GGT5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GGT5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GGT5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS940420
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NCBI/Uniprot data below describe general gene information for GGT5. It may not necessarily be applicable to this product.
 NCBI GI #    31542891
 NCBI GeneID    23887
 NCBI Accession #    NP_035950.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_011820.4 [Other Products]
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 UniProt Primary Accession #    Q9Z2A9 [Other Products]
 UniProt Secondary Accession #    Q8C7B4 [Other Products]
 UniProt Related Accession #    Q9Z2A9 [Other Products]
 Molecular Weight    61,674 Da
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 NCBI Official Full Name    gamma-glutamyltransferase 5
 NCBI Official Synonym Full Names    gamma-glutamyltransferase 5
 NCBI Official Symbol    Ggt5 [Similar Products]
 NCBI Official Synonym Symbols   
GGL; Ggtla1; GGT-REL; AI551243
[Similar Products]
 NCBI Protein Information    gamma-glutamyltransferase 5; GGT 5; glutathione hydrolase 5; leukotriene-C4 hydrolase; gamma-glutamyl leukotrienase; gamma-glutamyltranspeptidase 5; gamma-glutamyltransferase-like activity 1
 UniProt Protein Name    Gamma-glutamyltransferase 5
 UniProt Synonym Protein Names   
Gamma-glutamyl leukotrienase; GGL; Gamma-glutamyltransferase-like activity 1; Gamma-glutamyltranspeptidase 5; Glutathione hydrolase 5 (EC:3.4.19.13); Leukotriene-C4 hydrolase (EC:3.4.19.14)Cleaved into the following 2 chains:Gamma-glutamyltransferase 5 heavy chain; Gamma-glutamyltransferase 5 light chain
 Protein Family    Gamma-glutamyltransferase
 UniProt Gene Name    Ggt5 [Similar Products]
 UniProt Synonym Gene Names    Ggtla1; GGT 5; GGL [Similar Products]
 UniProt Entry Name    GGT5_MOUSE
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 UniProt Comments for GGT5    GGT5: Cleaves the gamma-glutamyl peptide bond of glutathione conjugates, but maybe not glutathione itself. Converts leukotriene C4 (LTC4) to leukotriene D4 (LTD4). Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.13; EC 3.4.19.14; Other Amino Acids Metabolism - glutathione; Membrane protein, integral; Other Amino Acids Metabolism - selenoamino acid; Other Amino Acids Metabolism - cyanoamino acid; Lipid Metabolism - arachidonic acid; Transferase; Other Amino Acids Metabolism - taurine and hypotaurine; EC 2.3.2.2

Cellular Component: membrane; integral to membrane; plasma membrane

Molecular Function: peptidase activity; transferase activity; hydrolase activity; gamma-glutamyltransferase activity; transferase activity, transferring acyl groups

Biological Process: leukotriene biosynthetic process; glutathione metabolic process; glutathione biosynthetic process; proteolysis; inflammatory response
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 Research Articles on GGT5    1. plays an important role in inflammatory processes
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with GGT5 elisa kitOrgans/Tissues associated with GGT5 elisa kit
 Products by Pathway  Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Via LOX (Lipooxygenase) Pathway antibodies  Arachidonic Acid Metabolism Via LOX (Lipooxygenase) Pathway Diagram
 Cyanoamino Acid Metabolism Pathway antibodies  Cyanoamino Acid Metabolism Pathway Diagram
 Cyanoamino Acid Metabolism Pathway antibodies  Cyanoamino Acid Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Leukotriene Synthesis Pathway antibodies  Leukotriene Synthesis Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >1 publications with GGT5 and Skin
 Uncharacterized Tissue Antibodies  >1 publications with GGT5 and Uncharacterized Tissue
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