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GLP1R elisa kit :: Mouse glucagon-like peptide 1 receptor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS930494
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

glucagon-like peptide 1 receptor (GLP1R), ELISA Kit

 Also Known As   

Mouse Glucagon-like peptide 1 receptor, GLP1R ELISA Kit

 Product Synonym Names    Mouse Glucagon-like peptide 1 receptor (GLP1R) ELISA kit; MGC138331; ; glucagon-like peptide 1 receptor
 Product Gene Name   

GLP1R elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS930494 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for O35659
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse GLP1R. No significant cross-reactivity or interference between mouse GLP1R and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    23.44 pg/ml-1500 pg/ml.
 Sensitivity    The minimum detectable dose of mouse GLP1R is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GLP1R elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GLP1R purchase    MBS930494 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glucagon-like peptide 1 receptor (GLP1R) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 23.44 pg/ml-1500 pg/ml in biological research samples containing GLP1R, with an estimated sensitivity of The minimum detectable dose of mouse GLP1R is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS930494 kit is based on GLP1R antibody-GLP1R antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GLP1R antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GLP1R. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GLP1R elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GLP1R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GLP1R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLP1R is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLP1R bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of GLP1R elisa kit    GLP1R elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS930494. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GLP1R. It may not necessarily be applicable to this product.
 NCBI GI #    111378379
 NCBI GeneID    14652
 NCBI Accession #    NP_067307.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_021332.2 [Other Products]
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 UniProt Primary Accession #    O35659 [Other Products]
 UniProt Secondary Accession #    Q1JQR8 [Other Products]
 UniProt Related Accession #    O35659 [Other Products]
 Molecular Weight    53,028 Da
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 NCBI Official Full Name    glucagon-like peptide 1 receptor
 NCBI Official Synonym Full Names    glucagon-like peptide 1 receptor
 NCBI Official Symbol    Glp1r [Similar Products]
 NCBI Official Synonym Symbols   
GLP-1R; GLP1Rc
[Similar Products]
 NCBI Protein Information    glucagon-like peptide 1 receptor; GLP-1-R; GLP-1 receptor
 UniProt Protein Name    Glucagon-like peptide 1 receptor
 Protein Family    Glucagon-like peptide 1 receptor
 UniProt Gene Name    Glp1r [Similar Products]
 UniProt Synonym Gene Names    GLP-1 receptor; GLP-1-R; GLP-1R [Similar Products]
 UniProt Entry Name    GLP1R_MOUSE
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 UniProt Comments for GLP1R    GLP1R: This is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 2 family.

Protein type: Membrane protein, integral; GPCR, family 2; Receptor, GPCR; Membrane protein, multi-pass

Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane; intracellular; cytosol

Molecular Function: G-protein coupled receptor activity; signal transducer activity; transmembrane receptor activity; peptide receptor activity; peptide hormone binding; peptide receptor activity, G-protein coupled; glucagon receptor activity; receptor activity

Biological Process: hormone secretion; positive regulation of insulin secretion; regulation of calcium ion transport; signal transduction; G-protein signaling, adenylate cyclase activating pathway; memory; regulation of heart contraction; G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; learning and/or memory; insulin secretion; neuropeptide signaling pathway; release of sequestered calcium ion into cytosol; positive regulation of cell proliferation; response to glucose stimulus; response to stress; positive regulation of transcription from RNA polymerase II promoter; feeding behavior; negative regulation of neuron apoptosis; positive regulation of cell differentiation; positive regulation of heart contraction; associative learning; positive regulation of blood pressure; negative regulation of apoptosis
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 Research Articles on GLP1R    1. GLP-1R is shown to be a recycling receptor.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with GLP1R elisa kitDiseases associated with GLP1R elisa kit
 Products by Pathway  Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class B Secretin-like Pathway antibodies  GPCRs, Class B Secretin-like Pathway Diagram
 Glucagon-like Peptide-1 (GLP1) Regulates Insulin Secretion Pathway antibodies  Glucagon-like Peptide-1 (GLP1) Regulates Insulin Secretion Pathway Diagram
 Glucagon-type Ligand Receptors Pathway antibodies  Glucagon-type Ligand Receptors Pathway Diagram
 Insulin Secretion Pathway antibodies  Insulin Secretion Pathway Diagram
 Integration Of Energy Metabolism Pathway antibodies  Integration Of Energy Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Obesity Antibodies  >28 publications with GLP1R and Obesity
 Diabetes Mellitus, Type 2 Antibodies  >17 publications with GLP1R and Diabetes Mellitus, Type 2
 Hyperglycemia Antibodies  >16 publications with GLP1R and Hyperglycemia
 Weight Loss Antibodies  >11 publications with GLP1R and Weight Loss
 Insulin Resistance Antibodies  >9 publications with GLP1R and Insulin Resistance
 Glucose Intolerance Antibodies  >7 publications with GLP1R and Glucose Intolerance
 Disease Models, Animal Antibodies  >7 publications with GLP1R and Disease Models, Animal
 Vascular Diseases Antibodies  >7 publications with GLP1R and Vascular Diseases
 Brain Diseases Antibodies  >6 publications with GLP1R and Brain Diseases
 Heart Diseases Antibodies  >4 publications with GLP1R and Heart Diseases
Organs/Tissues associated with GLP1R elisa kit
 Organ/Tissue Name  Pubmed Publications
 Mixed Antibodies  >1 publications with GLP1R and Mixed
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