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GCG elisa kit :: Mouse glucagon ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705540
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

glucagon (GCG), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Glucagon, GC ELISA Kit

 Product Synonym Names    Mouse Glucagon (GC) ELISA Kit; GLP1; GLP2; GRPP; glicentin-related polypeptide; glucagon-like peptide 1; glucagon-like peptide 2; glucagon
 Product Gene Name   

GCG elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705540 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P55095
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse GCG. No significant cross-reactivity or interference between Mouse GCG and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    1.56 pg/ml-100 pg/ml
 Sensitivity    0.39 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GCG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GCG purchase    MBS705540 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glucagon (GCG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56 pg/ml-100 pg/ml in biological research samples containing GCG, with an estimated sensitivity of 0.39 pg/ml. The ELISA analytical biochemical technique of the MBS705540 kit is based on GCG antibody-GCG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GCG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GCG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GCG elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GC bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of GCG elisa kit    GCG elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705540. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GCG. It may not necessarily be applicable to this product.
 NCBI GI #    33468853
 NCBI GeneID    14526
 NCBI Accession #    NP_032126.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_008100.3 [Other Products]
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 UniProt Primary Accession #    P55095 [Other Products]
 UniProt Secondary Accession #    Q3UFE9 [Other Products]
 UniProt Related Accession #    P55095 [Other Products]
 Molecular Weight    20,906 Da
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 NCBI Official Full Name    glucagon
 NCBI Official Synonym Full Names    glucagon
 NCBI Official Symbol    Gcg [Similar Products]
 NCBI Official Synonym Symbols   
Glu; PPG; GLP-1
[Similar Products]
 NCBI Protein Information    glucagon; glucagon-like peptide I
 UniProt Protein Name    Glucagon
 Protein Family    Glucagon
 UniProt Gene Name    Gcg [Similar Products]
 UniProt Synonym Gene Names    GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2 [Similar Products]
 UniProt Entry Name    GLUC_MOUSE
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 NCBI Summary for GCG    This gene encodes glucagon, a pancreatic hormone that counteracts the action of insulin in the bloodstream. The encoded protein is processed to generate glucagon and two other glucagon-like peptides, GLP1 and GLP2. Glucagon stimulates gluconeogenesis, glycogenolysis and lipolysis. GLP1 induces secretion of insulin, suppresses glucagon secretion and inhibits feeding. GLP2 induces intestinal absorption of glucose by stimulating the growth of intestinal cells and preventing apoptosis. [provided by RefSeq, Apr 2015]
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 UniProt Comments for GCG    Function: Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia 1 PublicationManual assertion based on experiment in:Ref.5
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 Research Articles on GCG    1. Glutamine stimulates the co-release of endogenous GLP-1 and PYY from mucosal L-cells resulting in paracrine GLP-1 and Y1 receptor-mediated electrogenic epithelial responses.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with GCG elisa kitDiseases associated with GCG elisa kit
 Products by Pathway  Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Glucagon Signaling In Metabolic Regulation Pathway antibodies  Glucagon Signaling In Metabolic Regulation Pathway Diagram
 Glucagon-like Peptide-1 (GLP1) Regulates Insulin Secretion Pathway antibodies  Glucagon-like Peptide-1 (GLP1) Regulates Insulin Secretion Pathway Diagram
 Glucagon-type Ligand Receptors Pathway antibodies  Glucagon-type Ligand Receptors Pathway Diagram
 Incretin Synthesis, Secretion, And Inactivation Pathway antibodies  Incretin Synthesis, Secretion, And Inactivation Pathway Diagram
 Disease Name  Pubmed Publications
 Muscular Diseases Antibodies  >72 publications with GCG and Muscular Diseases
 Diabetes Mellitus, Type 2 Antibodies  >24 publications with GCG and Diabetes Mellitus, Type 2
 Vascular Diseases Antibodies  >15 publications with GCG and Vascular Diseases
 Obesity Antibodies  >14 publications with GCG and Obesity
 Insulin Resistance Antibodies  >12 publications with GCG and Insulin Resistance
 Kidney Diseases Antibodies  >11 publications with GCG and Kidney Diseases
 Hyperglycemia Antibodies  >10 publications with GCG and Hyperglycemia
 Hyperinsulinism Antibodies  >9 publications with GCG and Hyperinsulinism
 Heart Diseases Antibodies  >8 publications with GCG and Heart Diseases
 Liver Diseases Antibodies  >7 publications with GCG and Liver Diseases
Organs/Tissues associated with GCG elisa kit
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >17 publications with GCG and Pancreas
 Intestine Antibodies  >11 publications with GCG and Intestine
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