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GAD1 elisa kit :: Mouse glutamate decarboxylase 1 (brain, 67kDa) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS925594
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

glutamate decarboxylase 1 (brain, 67kDa) (GAD1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Glutamate decarboxylase 1, GAD1 ELISA Kit

 Product Synonym Names    Mouse Glutamate decarboxylase 1 (GAD1) ELISA kit; FLJ45882; GAD; SCP; glutamate decarboxylase 1; glutamate decarboxylase 1 (brain; 67kDa)
 Product Gene Name   

GAD1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS925594 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P48318
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse GAD1. No significant cross-reactivity or interference between mouse GAD1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates
 Assay Type    Quantitative Sandwich
 Detection Range    6.25 pg/ml-400 pg/ml
 Sensitivity    <1.56 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GAD1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GAD1 purchase    MBS925594 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glutamate decarboxylase 1 (brain, 67kDa) (GAD1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 pg/ml-400 pg/ml in biological research samples containing GAD1, with an estimated sensitivity of <1.56 pg/ml. The ELISA analytical biochemical technique of the MBS925594 kit is based on GAD1 antibody-GAD1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GAD1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GAD1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GAD1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GAD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAD1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of GAD1 elisa kit    GAD1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS925594. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GAD1. It may not necessarily be applicable to this product.
 NCBI GI #    31982847
 NCBI GeneID    14415
 NCBI Accession #    NP_032103.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_008077.4 [Other Products]
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 UniProt Primary Accession #    P48318 [Other Products]
 UniProt Secondary Accession #    O08685 [Other Products]
 UniProt Related Accession #    P48318 [Other Products]
 Molecular Weight    66,648 Da
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 NCBI Official Full Name    glutamate decarboxylase 1
 NCBI Official Synonym Full Names    glutamate decarboxylase 1
 NCBI Official Symbol    Gad1 [Similar Products]
 NCBI Official Synonym Symbols   
EP10; GAD25; GAD44; GAD67; Gad-1
[Similar Products]
 NCBI Protein Information    glutamate decarboxylase 1; GAD-67; glutamic acid decarboxylase 1; 67 kDa glutamic acid decarboxylase; glutamate decarboxylase 67 kDa isoform; GLUTAMATE DECARBOXYLASE, 67 KD ISOFORM (GAD-67) (67 KD GLUTAMIC ACID DECARBOXYLASE)
 UniProt Protein Name    Glutamate decarboxylase 1
 UniProt Synonym Protein Names   
67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform
 Protein Family    Glutamate decarboxylase
 UniProt Gene Name    Gad1 [Similar Products]
 UniProt Synonym Gene Names    Gad67; GAD-67 [Similar Products]
 UniProt Entry Name    DCE1_MOUSE
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 NCBI Summary for GAD1    The protein encoded by this gene is an enzyme that catalyzes the production of gamma-aminobutyric acid from L-glutamic acid. Alternative splicing of this gene results in two products, the predominant 67 kDa isoform and a smaller 25 kDa isoform. The smaller isoform is not thought to retain catalytic activity. [provided by RefSeq, Aug 2015]
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 UniProt Comments for GAD1    GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Other Amino Acids Metabolism - taurine and hypotaurine; Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; EC 4.1.1.15; Carbohydrate Metabolism - butanoate; Other Amino Acids Metabolism - beta-alanine; Lyase

Cellular Component: mitochondrion; axon; cytoplasm; presynaptic active zone; synapse; cell cortex; intracellular; nerve terminal

Molecular Function: glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; lyase activity; carboxy-lyase activity; protein N-terminus binding; catalytic activity; pyridoxal phosphate binding

Biological Process: gamma-aminobutyric acid biosynthetic process; carboxylic acid metabolic process; social behavior; neurotransmitter biosynthetic process
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 Research Articles on GAD1    1. Sex differences in reproductive genes Gad1, Kiss1 and Gnrh1 are critically disturbed in GABAB1-deficient mice, probably altering the organization and development of neural circuits governing the reproductive axis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with GAD1 elisa kitDiseases associated with GAD1 elisa kit
 Products by Pathway  Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABAergic Synapse Pathway antibodies  GABAergic Synapse Pathway Diagram
 GABAergic Synapse Pathway antibodies  GABAergic Synapse Pathway Diagram
 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >42 publications with GAD1 and Mental Disorders
 Schizophrenia Antibodies  >27 publications with GAD1 and Schizophrenia
 Nervous System Diseases Antibodies  >22 publications with GAD1 and Nervous System Diseases
 Brain Diseases Antibodies  >17 publications with GAD1 and Brain Diseases
 Mood Disorders Antibodies  >11 publications with GAD1 and Mood Disorders
 Bipolar Disorder Antibodies  >11 publications with GAD1 and Bipolar Disorder
 Disease Models, Animal Antibodies  >10 publications with GAD1 and Disease Models, Animal
 Substance-Related Disorders Antibodies  >8 publications with GAD1 and Substance-Related Disorders
 Epilepsy Antibodies  >8 publications with GAD1 and Epilepsy
 Seizures Antibodies  >7 publications with GAD1 and Seizures
Organs/Tissues associated with GAD1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >65 publications with GAD1 and Brain
 Spinal Cord Antibodies  >4 publications with GAD1 and Spinal Cord
 Embryonic Tissue Antibodies  >1 publications with GAD1 and Embryonic Tissue
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