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GOT1 elisa kit :: Mouse glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS904927 GOT1 elisa kit
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1), ELISA Kit

 Also Known As   

Mouse Aspartate aminotransferase, cytoplasmic, GOT1 ELISA Kit

 Product Synonym Names    Mouse Aspartate aminotransferase; cytoplasmic (GOT1) ELISA kit; GIG18; aspartate aminotransferase 1; growth-inhibiting protein 18; glutamic-oxaloacetic transaminase 1; soluble (aspartate aminotransferase 1)
 Product Gene Name   

GOT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P05201
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse GOT1. No significant cross-reactivity or interference between Mouse GOT1 and analogues was observed.
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 Assay Type    Sandwich
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GOT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GOT1 purchase    MBS904927 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GOT1. The ELISA analytical biochemical technique of the MBS904927 kit is based on GOT1 antibody-GOT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GOT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GOT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GOT1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GOT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GOT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GOT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GOT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS904927. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GOT1. It may not necessarily be applicable to this product.
 NCBI GI #    160298209
 NCBI GeneID    14718
 NCBI Accession #    NP_034454.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_010324.2 [Other Products]
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 UniProt Primary Accession #    P05201 [Other Products]
 UniProt Secondary Accession #    Q3UJH8 [Other Products]
 UniProt Related Accession #    P05201 [Other Products]
 Molecular Weight    46,248 Da
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 NCBI Official Full Name    aspartate aminotransferase, cytoplasmic
 NCBI Official Synonym Full Names    glutamate oxaloacetate transaminase 1, soluble
 NCBI Official Symbol    Got1 [Similar Products]
 NCBI Official Synonym Symbols   
cCAT; Got-1; cAspAT; AI789014
[Similar Products]
 NCBI Protein Information    aspartate aminotransferase, cytoplasmic; transaminase A; cysteine transaminase, cytoplasmic; cytosolic aspartate aminotransferase; cysteine aminotransferase, cytoplasmic
 UniProt Protein Name    Aspartate aminotransferase, cytoplasmic
 UniProt Synonym Protein Names   
Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A
 Protein Family    Protein transport protein
 UniProt Gene Name    Got1 [Similar Products]
 UniProt Synonym Gene Names    cAspAT; cCAT [Similar Products]
 UniProt Entry Name    AATC_MOUSE
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 UniProt Comments for GOT1    GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: EC 2.6.1.3; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; EC 2.6.1.1; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; Amino Acid Metabolism - tyrosine

Cellular Component: lysosome; cytoplasm; nerve terminal; nucleus; cytosol

Molecular Function: transferase activity; cysteine transaminase activity; carboxylic acid binding; phosphatidylserine decarboxylase activity; aspartate transaminase activity; transaminase activity; catalytic activity; pyridoxal phosphate binding

Biological Process: amino acid metabolic process; dicarboxylic acid metabolic process; oxaloacetate metabolic process; glycerol biosynthetic process; glutamate catabolic process to aspartate; fatty acid homeostasis; aspartate biosynthetic process; aspartate metabolic process; biosynthetic process; aspartate catabolic process; amino acid biosynthetic process; glutamate catabolic process to 2-oxoglutarate
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 Research Articles on GOT1    1. cAspAT is a new member of glyceroneogenesis, transcriptionally regulated by TZD via the control of RORalpha expression by PPARgamma in adipocytes
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with GOT1 elisa kitDiseases associated with GOT1 elisa kit
 Products by Pathway  Pathway Diagram
 4-hydroxyproline Degradation I Pathway antibodies  4-hydroxyproline Degradation I Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >19 publications with GOT1 and Neoplasms
 Carcinoma Antibodies  >14 publications with GOT1 and Carcinoma
 Adenocarcinoma Antibodies  >11 publications with GOT1 and Adenocarcinoma
 Disease Models, Animal Antibodies  >4 publications with GOT1 and Disease Models, Animal
 Nervous System Diseases Antibodies  >3 publications with GOT1 and Nervous System Diseases
 Pancreatic Neoplasms Antibodies  >2 publications with GOT1 and Pancreatic Neoplasms
 Breast Neoplasms Antibodies  >2 publications with GOT1 and Breast Neoplasms
 Liver Neoplasms Antibodies  >2 publications with GOT1 and Liver Neoplasms
 Liver Diseases Antibodies  >2 publications with GOT1 and Liver Diseases
 Neoplasms, Experimental Antibodies  >2 publications with GOT1 and Neoplasms, Experimental
Organs/Tissues associated with GOT1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11 publications with GOT1 and Blood
 Heart Antibodies  >4 publications with GOT1 and Heart
 Muscle Antibodies  >3 publications with GOT1 and Muscle
 Intestine Antibodies  >2 publications with GOT1 and Intestine
 Spinal Cord Antibodies  >2 publications with GOT1 and Spinal Cord
 Lung Antibodies  >2 publications with GOT1 and Lung
 Bone Antibodies  >2 publications with GOT1 and Bone
 Liver Antibodies  >2 publications with GOT1 and Liver
 Brain Antibodies  >2 publications with GOT1 and Brain
 Testis Antibodies  >1 publications with GOT1 and Testis
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