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GPC4 elisa kit :: Mouse glypican 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS918853 GPC4 elisa kit
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

glypican 4 (GPC4), ELISA Kit

 Also Known As   

Mouse Glypican-4, GPC4 ELISA Kit

 Product Synonym Names    Mouse Glypican-4 (GPC4) ELISA kit; RP6-198C21.1; K-glypican; dJ900E8.1 (glypican 4) ; glypican proteoglycan 4; glypican 4
 Product Gene Name   

GPC4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P51655
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse GPC4. No significant cross-reactivity or interference between mouse GPC4 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    62.5 pg/ml-4000 pg/ml.
 Sensitivity    The minimum detectable dose of mouse GPC4 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GPC4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GPC4 purchase    MBS918853 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glypican 4 (GPC4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 pg/ml-4000 pg/ml in biological research samples containing GPC4, with an estimated sensitivity of The minimum detectable dose of mouse GPC4 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS918853 kit is based on GPC4 antibody-GPC4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPC4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPC4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for GPC4 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GPC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPC4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPC4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS918853. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GPC4. It may not necessarily be applicable to this product.
 NCBI GI #    238624186
 NCBI GeneID    14735
 NCBI Accession #    NP_032176.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_008150.2 [Other Products]
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 UniProt Primary Accession #    P51655 [Other Products]
 UniProt Secondary Accession #    Q923C8 [Other Products]
 UniProt Related Accession #    P51655 [Other Products]
 Molecular Weight    62,586 Da
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 NCBI Official Full Name    glypican-4
 NCBI Official Synonym Full Names    glypican 4
 NCBI Official Symbol    Gpc4 [Similar Products]
 NCBI Official Synonym Symbols   
AF311610; AI385680; AI661372; K-glypican; 9530073D23Rik
[Similar Products]
 NCBI Protein Information    glypican-4
 UniProt Protein Name    Glypican-4
 UniProt Synonym Protein Names   
K-glypicanCleaved into the following chain:Secreted glypican-4
 Protein Family    Glypican
 UniProt Gene Name    Gpc4 [Similar Products]
 UniProt Entry Name    GPC4_MOUSE
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 UniProt Comments for GPC4    GPC4: Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system. Belongs to the glypican family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor

Cellular Component: proteinaceous extracellular matrix; membrane; extracellular region; plasma membrane; nucleus; external side of plasma membrane

Molecular Function: heparan sulfate proteoglycan binding
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 Research Articles on GPC4    1. Findings establish that Gpc4 acts at the interface of extrinsic and intrinsic signal regulation to fine tune stem cell fate.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with GPC4 elisa kitDiseases associated with GPC4 elisa kit
 Products by Pathway  Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4 publications with GPC4 and Neoplasms
 Heart Defects, Congenital Antibodies  >2 publications with GPC4 and Heart Defects, Congenital
 Disease Models, Animal Antibodies  >2 publications with GPC4 and Disease Models, Animal
 Nervous System Diseases Antibodies  >1 publications with GPC4 and Nervous System Diseases
 Kidney Diseases Antibodies  >1 publications with GPC4 and Kidney Diseases
 Ovarian Neoplasms Antibodies  >1 publications with GPC4 and Ovarian Neoplasms
 Obesity Antibodies  >1 publications with GPC4 and Obesity
 Liver Diseases Antibodies  >1 publications with GPC4 and Liver Diseases
 Uterine Cervical Neoplasms Antibodies  >1 publications with GPC4 and Uterine Cervical Neoplasms
 Hydrocephalus Antibodies  >1 publications with GPC4 and Hydrocephalus
Organs/Tissues associated with GPC4 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >6 publications with GPC4 and Kidney
 Brain Antibodies  >6 publications with GPC4 and Brain
 Embryonic Tissue Antibodies  >5 publications with GPC4 and Embryonic Tissue
 Heart Antibodies  >3 publications with GPC4 and Heart
 Lung Antibodies  >2 publications with GPC4 and Lung
 Intestine Antibodies  >1 publications with GPC4 and Intestine
 Eye Antibodies  >1 publications with GPC4 and Eye
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