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GZMB elisa kit :: Mouse granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS701504
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $575 +1 FREE 8GB USB
96-Strip-Wells  /  $835 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) (GZMB), ELISA Kit

 Also Known As   

Mouse granzymes B, Gzms-B ELISA Kit

 Product Synonym Names    Mouse granzymes B; Gzms-B ELISA Kit; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; HLP; SECT; T-cell serine protease 1-3E; cathepsin G-like 1; cytotoxic serine protease B; fragmentin 2; granzyme B; granzyme B (granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1)
 Product Gene Name   

GZMB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS701504 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P04187
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse GZMB. No significant cross-reactivity or interference between Mouse GZMB and analogues was observed.
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 Detection Range    0.32 ng/ml-20 ng/ml
 Sensitivity    0.08 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Apoptosis/Autophagy
 Protein Biological Process 3    Apoptosis
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GZMB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GZMB purchase    MBS701504 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) (GZMB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.32 ng/ml-20 ng/ml in biological research samples containing GZMB, with an estimated sensitivity of 0.08 ng/ml. The ELISA analytical biochemical technique of the MBS701504 kit is based on GZMB antibody-GZMB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GZMB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GZMB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for GZMB elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gzms-B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Gzms-B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Gzms-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Gzms-B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of GZMB elisa kit    GZMB elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS701504. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GZMB. It may not necessarily be applicable to this product.
 NCBI GI #    7305123
 NCBI GeneID    14939
 NCBI Accession #    NP_038570.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_013542.2 [Other Products]
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 UniProt Primary Accession #    P04187 [Other Products]
 UniProt Related Accession #    P04187 [Other Products]
 Molecular Weight    27,470 Da
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 NCBI Official Full Name    granzyme B(G,H)
 NCBI Official Synonym Full Names    granzyme B
 NCBI Official Symbol    Gzmb [Similar Products]
 NCBI Official Synonym Symbols   
GZB; CCP1; Ctla1; Ctla-1; AI553453; CCP-1/C11
[Similar Products]
 NCBI Protein Information    granzyme B(G,H); granzyme B(G,H); fragmentin-2; cytotoxic cell protease 1
 UniProt Protein Name    Granzyme B(G,H)
 UniProt Synonym Protein Names   
CTLA-1; Cytotoxic cell protease 1; CCP1; Fragmentin-2
 Protein Family    Granzyme
 UniProt Gene Name    Gzmb [Similar Products]
 UniProt Synonym Gene Names    Ctla-1; Ctla1; CCP1 [Similar Products]
 UniProt Entry Name    GRAB_MOUSE
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 UniProt Comments for GZMB    Function: This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis

Catalytic activity: Preferential cleavage: -Asp-|-Xaa- >> -Asn-|-Xaa- > -Met-|-Xaa-, -Ser-|-Xaa-.
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 Research Articles on GZMB    1. GzmB is a negative regulator of hematopoietic stem cell function that is induced by stress and chemotherapy in both HSCs and their niches.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GZMB elisa kitPathways associated with GZMB elisa kit
 Reference Product  PubMed Publications
 CASP3 elisa kit  >87 publications with GZMB and CASP3
 BID elisa kit  >41 publications with GZMB and BID
 SERPINB9 elisa kit  >36 publications with GZMB and SERPINB9
 CASP9 elisa kit  >22 publications with GZMB and CASP9
 PRF1 elisa kit  >14 publications with GZMB and PRF1
 CASP7 elisa kit  >13 publications with GZMB and CASP7
 PRKDC elisa kit  >3 publications with GZMB and PRKDC
 XRCC4 elisa kit  >1 publications with GZMB and XRCC4
 UBE4B elisa kit  >1 publications with GZMB and UBE4B
 RAB27A elisa kit  >1 publications with GZMB and RAB27A
 Products by Pathway  Pathway Diagram
 Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway antibodies  Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Graft-versus-host Disease Pathway antibodies  Graft-versus-host Disease Pathway Diagram
 Graft-versus-host Disease Pathway antibodies  Graft-versus-host Disease Pathway Diagram
 Intrinsic Pathway For Apoptosis antibodies  Intrinsic Pathway For Apoptosis Diagram
Diseases associated with GZMB elisa kitOrgans/Tissues associated with GZMB elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >303 publications with GZMB and Necrosis
 Inflammation Antibodies  >192 publications with GZMB and Inflammation
 Disease Models, Animal Antibodies  >112 publications with GZMB and Disease Models, Animal
 Nervous System Diseases Antibodies  >95 publications with GZMB and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >95 publications with GZMB and Neoplasms, Experimental
 Liver Diseases Antibodies  >74 publications with GZMB and Liver Diseases
 Hepatitis Antibodies  >54 publications with GZMB and Hepatitis
 Brain Diseases Antibodies  >35 publications with GZMB and Brain Diseases
 Breast Neoplasms Antibodies  >33 publications with GZMB and Breast Neoplasms
 Kidney Diseases Antibodies  >27 publications with GZMB and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >806 publications with GZMB and Blood
 Kidney Antibodies  >80 publications with GZMB and Kidney
 Lung Antibodies  >62 publications with GZMB and Lung
 Liver Antibodies  >61 publications with GZMB and Liver
 Spleen Antibodies  >56 publications with GZMB and Spleen
 Bone Marrow Antibodies  >44 publications with GZMB and Bone Marrow
 Heart Antibodies  >32 publications with GZMB and Heart
 Intestine Antibodies  >28 publications with GZMB and Intestine
 Pancreas Antibodies  >13 publications with GZMB and Pancreas
 Stomach Antibodies  >10 publications with GZMB and Stomach
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