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HSPA12B elisa kit :: Mouse heat shock 70kD protein 12B ELISA Kit

Scan QR to view Datasheet Catalog #    MBS917640
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

heat shock 70kD protein 12B (HSPA12B), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Heat shock 70 kDa protein 12B, HSPA12B ELISA Kit

 Product Synonym Names    Mouse Heat shock 70 kDa protein 12B (HSPA12B) ELISA kit; RP5-1009E24.4; C20orf60; FLJ32150; MGC131912; dJ1009E24.2; ; heat shock 70kD protein 12B
 Product Gene Name   

HSPA12B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS917640 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q9CZJ2
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse HSPA12B. No significant cross-reactivity or interference between mouse HSPA12B and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates
 Assay Type    Quantitative Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml
 Sensitivity    Typically less than 7.8 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HSPA12B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HSPA12B purchase    MBS917640 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the heat shock 70kD protein 12B (HSPA12B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing HSPA12B, with an estimated sensitivity of Typically less than 7.8 pg/ml. The ELISA analytical biochemical technique of the MBS917640 kit is based on HSPA12B antibody-HSPA12B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSPA12B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSPA12B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for HSPA12B elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSPA12B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSPA12B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSPA12B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSPA12B bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of HSPA12B elisa kit    HSPA12B elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS917640. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HSPA12B. It may not necessarily be applicable to this product.
 NCBI GI #    21312236
 NCBI GeneID    72630
 NCBI Accession #    NP_082582.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_028306.3 [Other Products]
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 UniProt Primary Accession #    Q9CZJ2 [Other Products]
 UniProt Related Accession #    Q9CZJ2 [Other Products]
 Molecular Weight    76,119 Da
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 NCBI Official Full Name    heat shock 70 kDa protein 12B
 NCBI Official Synonym Full Names    heat shock protein 12B
 NCBI Official Symbol    Hspa12b [Similar Products]
 NCBI Official Synonym Symbols   
2700081N06Rik
[Similar Products]
 NCBI Protein Information    heat shock 70 kDa protein 12B
 UniProt Protein Name    Heat shock 70 kDa protein 12B
 Protein Family    Heat shock 70 kDa protein
 UniProt Gene Name    Hspa12b [Similar Products]
 UniProt Entry Name    HS12B_MOUSE
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 UniProt Comments for HSPA12B    HSPA12B: contains an atypical heat shock protein 70 (Hsp70) ATPase domain and is therefore a distant member of the mammalian Hsp70 family. This gene may be involved in susceptibility to atherosclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2010]

Protein type: Heat shock protein

Molecular Function: nucleotide binding; ATP binding
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 Research Articles on HSPA12B    1. inhibition of eNOS abrogated the protection of HSPA12B against cardiac dysfunction and remodelling after myocardial infarction
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with HSPA12B elisa kitOrgans/Tissues associated with HSPA12B elisa kit
 Disease Name  Pubmed Publications
 Cleft Palate Antibodies  >1 publications with HSPA12B and Cleft Palate
 Atherosclerosis Antibodies  >1 publications with HSPA12B and Atherosclerosis
 Arteriosclerosis Antibodies  >1 publications with HSPA12B and Arteriosclerosis
 Inflammation Antibodies  >1 publications with HSPA12B and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >3 publications with HSPA12B and Embryonic Tissue
 Brain Antibodies  >1 publications with HSPA12B and Brain
 Heart Antibodies  >1 publications with HSPA12B and Heart
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