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HSPA8 elisa kit :: Mouse heat shock 70kDa protein 8 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS926334
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

heat shock 70kDa protein 8 (HSPA8), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Heat shock cognate 71 kDa protein, HSPA8 ELISA Kit

 Product Synonym Names    Mouse Heat shock cognate 71 kDa protein (HSPA8) ELISA kit; HSC54; HSC70; HSC71; HSP71; HSP73; HSPA10; LAP1; MGC131511; MGC29929; NIP71; LPS-associated protein 1; N-myristoyltransferase inhibitor protein 71; constitutive heat shock protein 70; heat shock 70kD p; heat shock 70kDa protein 8
 Product Gene Name   

HSPA8 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS926334 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P63017
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse HSPA8. No significant cross-reactivity or interference between Mouse HSPA8 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    9.4 pg/ml-600 pg/ml
 Sensitivity    2.35 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Neurobiology
 Protein Biological Process 3    Stress response
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HSPA8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HSPA8 purchase    MBS926334 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the heat shock 70kDa protein 8 (HSPA8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 9.4 pg/ml-600 pg/ml in biological research samples containing HSPA8, with an estimated sensitivity of 2.35 pg/ml. The ELISA analytical biochemical technique of the MBS926334 kit is based on HSPA8 antibody-HSPA8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSPA8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSPA8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HSPA8 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSPA8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSPA8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSPA8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSPA8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of HSPA8 elisa kit    HSPA8 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS926334. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HSPA8. It may not necessarily be applicable to this product.
 NCBI GI #    31981690
 NCBI GeneID    15481
 NCBI Accession #    NP_112442.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_031165.4 [Other Products]
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 UniProt Primary Accession #    P63017 [Other Products]
 UniProt Secondary Accession #    P08109; P12225; Q3U6R0; Q3U764; Q3U7D7; Q3U7E2; Q3U9B4; Q3U9G0; Q3UGM0; Q5FWJ6; Q62373 [Other Products]
 UniProt Related Accession #    P63017 [Other Products]
 Molecular Weight    70,871 Da
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 NCBI Official Full Name    heat shock cognate 71 kDa protein
 NCBI Official Synonym Full Names    heat shock protein 8
 NCBI Official Symbol    Hspa8 [Similar Products]
 NCBI Official Synonym Symbols   
Hsc70; Hsc71; Hsc73; Hsp73; Hspa10; 2410008N15Rik
[Similar Products]
 NCBI Protein Information    heat shock cognate 71 kDa protein; heat shock cognate hsc73; heat shock 70kD protein 8; heat shock 70 kDa protein 8; heat shock protein cognate 70
 UniProt Protein Name    Heat shock cognate 71 kDa protein
 UniProt Synonym Protein Names   
Heat shock 70 kDa protein 8
 UniProt Gene Name    Hspa8 [Similar Products]
 UniProt Synonym Gene Names    Hsc70; Hsc73 [Similar Products]
 UniProt Entry Name    HSP7C_MOUSE
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 UniProt Comments for HSPA8    HSC70: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co- chaperones. Interacts with HSPH1/HSP105. Interacts with IRAK1BP1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with PACRG and TSC2. Interacts with BAG1. Interacts with SV40 VP1. Interacts with DNAJC7. Interacts with HERC5. Interacts with CITED1 (via N-terminus); the interaction suppresses the association of CITED1 to p300/CBP and Smad-mediated transcription transactivation. Constitutively synthesized. Ubiquitous. Belongs to the heat shock protein 70 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Chaperone; Heat shock protein; RNA-binding; Nucleolus

Cellular Component: extracellular space; neuron projection; focal adhesion; protein complex; terminal button; ribonucleoprotein complex; cytosol; spliceosome; synaptic vesicle; membrane; cell soma; cytoplasm; plasma membrane; intracellular; nucleus; myelin sheath; ubiquitin ligase complex

Molecular Function: protein binding; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; ubiquitin protein ligase binding; ATPase activity; unfolded protein binding; nucleotide binding; ATPase activity, coupled; ADP binding; ATP binding; receptor binding

Biological Process: positive regulation of nuclear mRNA splicing, via spliceosome; regulation of transcription, DNA-dependent; protein folding; transcription, DNA-dependent; chaperone cofactor-dependent protein folding; regulation of cell cycle; RNA splicing; protein refolding; mRNA processing; negative regulation of transcription, DNA-dependent
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 Research Articles on HSPA8    1. These findings indicate that wild type ILK and the non-oncogenic ILK(R211A) mutation comprise a cardioprotective module with Hsp/c70.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HSPA8 elisa kitPathways associated with HSPA8 elisa kit
 Reference Product  PubMed Publications
 Hspb1 elisa kit  >17 publications with HSPA8 and Hspb1
 Hsph1 elisa kit  >3 publications with HSPA8 and Hsph1
 Products by Pathway  Pathway Diagram
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 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
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 Golgi Associated Vesicle Biogenesis Pathway antibodies  Golgi Associated Vesicle Biogenesis Pathway Diagram
Diseases associated with HSPA8 elisa kitOrgans/Tissues associated with HSPA8 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >60 publications with HSPA8 and Neoplasms
 Nervous System Diseases Antibodies  >44 publications with HSPA8 and Nervous System Diseases
 Brain Diseases Antibodies  >30 publications with HSPA8 and Brain Diseases
 Cardiovascular Diseases Antibodies  >24 publications with HSPA8 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >22 publications with HSPA8 and Disease Models, Animal
 Heart Diseases Antibodies  >16 publications with HSPA8 and Heart Diseases
 Drug Toxicity Antibodies  >15 publications with HSPA8 and Drug Toxicity
 Necrosis Antibodies  >14 publications with HSPA8 and Necrosis
 Carcinoma Antibodies  >13 publications with HSPA8 and Carcinoma
 Fibrosis Antibodies  >13 publications with HSPA8 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >87 publications with HSPA8 and Blood
 Brain Antibodies  >83 publications with HSPA8 and Brain
 Muscle Antibodies  >46 publications with HSPA8 and Muscle
 Liver Antibodies  >40 publications with HSPA8 and Liver
 Heart Antibodies  >33 publications with HSPA8 and Heart
 Kidney Antibodies  >28 publications with HSPA8 and Kidney
 Lung Antibodies  >11 publications with HSPA8 and Lung
 Eye Antibodies  >11 publications with HSPA8 and Eye
 Bone Antibodies  >8 publications with HSPA8 and Bone
 Ovary Antibodies  >8 publications with HSPA8 and Ovary
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