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HS elisa kit :: Mouse heparan sulfate (HS) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS262183
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

heparan sulfate (HS), ELISA Kit

★Popular Item★
 Also Known As   

Mouse heparan sulfate (HS) ELISA Kit

 Product Gene Name   

HS elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    603244
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 Species Reactivity    Mouse
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    10 ug/ml-0.156 ug/ml
 Sensitivity    Up to 0.05 ug/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HS purchase    MBS262183 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the heparan sulfate (HS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 10 ug/ml-0.156 ug/ml in biological research samples containing HS, with an estimated sensitivity of Up to 0.05 ug/ml. The ELISA analytical biochemical technique of the MBS262183 kit is based on HS antibody-HS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HS elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse HS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of HS elisa kit    HS elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS262183. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HS. It may not necessarily be applicable to this product.
 NCBI GI #    4826764
 NCBI GeneID    9957
 NCBI Accession #    NP_005105.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005114.2 [Other Products]
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 UniProt Secondary Accession #    Q6PEY8; B3KUA6 [Other Products]
 UniProt Related Accession #    O14792 [Other Products]
 Molecular Weight    35,773 Da
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 NCBI Official Full Name    heparan sulfate glucosamine 3-O-sulfotransferase 1
 NCBI Official Synonym Full Names    heparan sulfate (glucosamine) 3-O-sulfotransferase 1
 NCBI Official Symbol    HS3ST1 [Similar Products]
 NCBI Official Synonym Symbols   
3OST; 3OST1
[Similar Products]
 NCBI Protein Information    heparan sulfate glucosamine 3-O-sulfotransferase 1; 3-OST-1; h3-OST-1; heparan sulfate 3-O-sulfotransferase 1; heparin-glucosamine 3-O-sulfotransferase; heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1
 UniProt Protein Name    Heparan sulfate glucosamine 3-O-sulfotransferase 1
 UniProt Synonym Protein Names   
Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1; 3-OST-1; Heparan sulfate 3-O-sulfotransferase 1; h3-OST-1
 UniProt Gene Name    HS3ST1 [Similar Products]
 UniProt Synonym Gene Names    3OST; 3OST1; 3-OST-1; Heparan sulfate 3-O-sulfotransferase 1; h3-OST-1 [Similar Products]
 UniProt Entry Name    HS3S1_HUMAN
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 NCBI Summary for HS    Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It possesses both heparan sulfate glucosaminyl 3-O-sulfotransferase activity, anticoagulant heparan sulfate conversion activity, and is a rate limiting enzyme for synthesis of anticoagulant heparan. This enzyme is an intraluminal Golgi resident protein. [provided by RefSeq, Jul 2008]
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 UniProt Comments for HS    HS3ST1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site. Belongs to the sulfotransferase 1 family.

Protein type: Transferase; EC 2.8.2.23; Glycan Metabolism - heparan sulfate biosynthesis

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: Golgi lumen; integral to membrane

Molecular Function: sulfotransferase activity; heparin-glucosamine 3-O-sulfotransferase activity

Biological Process: glycosaminoglycan biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis
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 Research Articles on HS    1. Golgi-targeted HS3st1 localizes in the Golgi and results in the formation of a single type of AT-binding site and high anti-factor Xa activity
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HS elisa kitPathways associated with HS elisa kit
 Reference Product  PubMed Publications
 GLCE elisa kit  >1 publications with HS and GLCE
 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram
 Glycosaminoglycan Biosynthesis - Heparan Sulfate / Heparin Pathway antibodies  Glycosaminoglycan Biosynthesis - Heparan Sulfate / Heparin Pathway Diagram
 Glycosaminoglycan Biosynthesis - Heparan Sulfate / Heparin Pathway antibodies  Glycosaminoglycan Biosynthesis - Heparan Sulfate / Heparin Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 HS-GAG Biosynthesis Pathway antibodies  HS-GAG Biosynthesis Pathway Diagram
 Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway antibodies  Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway Diagram
 MPS I - Hurler Syndrome Pathway antibodies  MPS I - Hurler Syndrome Pathway Diagram
 MPS II - Hunter Syndrome Pathway antibodies  MPS II - Hunter Syndrome Pathway Diagram
 MPS IIIA - Sanfilippo Syndrome A Pathway antibodies  MPS IIIA - Sanfilippo Syndrome A Pathway Diagram
Diseases associated with HS elisa kit
 Disease Name  Pubmed Publications
 Skin Diseases Antibodies  >2 publications with HS and Skin Diseases
 Neoplasm Metastasis Antibodies  >1 publications with HS and Neoplasm Metastasis
 Hyperplasia Antibodies  >1 publications with HS and Hyperplasia
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