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KL elisa kit :: Mouse klotho ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702609 KL elisa kit
Unit / Price
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,550 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,815 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

klotho, ELISA Kit

 Also Known As   

Mouse Klotho ELISA Kit

 Product Synonym Names    Mouse Klotho ELISA Kit; klotho
 Product Gene Name   

KL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS702609 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for O35082
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse klotho. No significant cross-reactivity or interference between mouse klotho and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates.
 Assay Type    Sandwich (Quantitative)
 Detection Range    3.12 pg/ml-200 pg/ml.
 Sensitivity    0.78 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of KL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for KL purchase    MBS702609 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the klotho, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards render a theoretical kit detection range of 3.12 pg/ml-200 pg/ml in biological research samples containing KL, with an estimated sensitivity of 0.78 pg/ml. The ELISA analytical biochemical technique of the MBS702609 kit is based on KL antibody-KL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KL. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for KL elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for klotho has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any klotho present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for klotho is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of klotho bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS702609
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NCBI/Uniprot data below describe general gene information for KL. It may not necessarily be applicable to this product.
 NCBI GI #    227330584
 NCBI GeneID    16591
 NCBI Accession #    NP_038851.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_013823.2 [Other Products]
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 UniProt Primary Accession #    O35082 [Other Products]
 UniProt Secondary Accession #    O70175; O70621; B2RR78 [Other Products]
 UniProt Related Accession #    O35082 [Other Products]
 Molecular Weight    116,398 Da
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 NCBI Official Full Name    klotho
 NCBI Official Synonym Full Names    klotho
 NCBI Official Symbol    Kl [Similar Products]
 NCBI Official Synonym Symbols   
alpha-kl
[Similar Products]
 NCBI Protein Information    klotho; secreted form of Klotho protein
 UniProt Protein Name    Klotho
 Protein Family    Klotho
 UniProt Gene Name    Kl [Similar Products]
 UniProt Entry Name    KLOT_MOUSE
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 UniProt Comments for KL    Function: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.6 PublicationsManual assertion based on experiment in:Ref.1

Catalytic activity: A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.2 PublicationsManual assertion based on experiment in:Ref.13

Enzyme regulation: Inhibited by D-saccharic acid 1,4-lactone and taurocholic acid.1 PublicationManual assertion based on experiment in:Ref.13
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 Research Articles on KL    1. The presence of Klotho-independent FGF23 effects in a Klotho-expressing target organ represents a paradigm shift in the conceptualization of FGF23 endocrine action
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Mouse Klotho ELISA Kit
 Catalog #  Product Name  Catalog #  Product Name
 MBS2080275  APC-Linked Polyclonal Antibody to Klotho Beta (KLb)  MBS7224528  Canine Beta-klotho (KLB) ELISA Kit
 MBS2002696  Polyclonal Antibody to Klotho (KL)  MBS052646  Mouse Klotho Beta ELISA Kit
 MBS2080298  FITC-Linked Polyclonal Antibody to Klotho (KL)  MBS2080297  Cy3-Linked Polyclonal Antibody to Klotho (KL)
 MBS2080268  FITC-Linked Monoclonal Antibody to Klotho Beta (KLb)  MBS044986  Canine Klotho ELISA Kit
 MBS691589  Human Klotho  MBS9369732  Mouse Soluble Alpha-Klotho (SAKL) ELISA Kit
Products associated with KL elisa kitPathways associated with KL elisa kit
 Reference Product  PubMed Publications
 Fgf23 elisa kit  >36 publications with KL and Fgf23
 Fgf15 elisa kit  >1 publications with KL and Fgf15
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway antibodies  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway Diagram
 Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway antibodies  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
Diseases associated with KL elisa kitOrgans/Tissues associated with KL elisa kit
 Disease Name  Pubmed Publications
 Pneumonia Antibodies  >206 publications with KL and Pneumonia
 Fibrosis Antibodies  >142 publications with KL and Fibrosis
 Skin Diseases Antibodies  >118 publications with KL and Skin Diseases
 Pulmonary Fibrosis Antibodies  >116 publications with KL and Pulmonary Fibrosis
 Inflammation Antibodies  >89 publications with KL and Inflammation
 Heart Diseases Antibodies  >73 publications with KL and Heart Diseases
 Lung Injury Antibodies  >59 publications with KL and Lung Injury
 Kidney Diseases Antibodies  >55 publications with KL and Kidney Diseases
 Necrosis Antibodies  >43 publications with KL and Necrosis
 Hypertension Antibodies  >36 publications with KL and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >377 publications with KL and Lung
 Bone Antibodies  >243 publications with KL and Bone
 Brain Antibodies  >196 publications with KL and Brain
 Kidney Antibodies  >93 publications with KL and Kidney
 Connective Tissue Antibodies  >51 publications with KL and Connective Tissue
 Eye Antibodies  >32 publications with KL and Eye
 Spleen Antibodies  >31 publications with KL and Spleen
 Intestine Antibodies  >19 publications with KL and Intestine
 Parathyroid Antibodies  >16 publications with KL and Parathyroid
 Ganglia Antibodies  >13 publications with KL and Ganglia
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