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MC5R elisa kit :: Mouse melanocortin 5 receptor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS904648
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

melanocortin 5 receptor (MC5R), ELISA Kit

 Also Known As   

Mouse Melanocortin receptor 5, MC5R ELISA Kit

 Product Synonym Names    Mouse Melanocortin receptor 5 (MC5R) ELISA kit; melanocortin receptor 5; melanocortin 5 receptor
 Product Gene Name   

MC5R elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS904648 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P41149
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse MC5R. No significant cross-reactivity or interference between Mouse MC5R and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    31.2 pg/ml-2000 pg/ml
 Sensitivity    7.8 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MC5R elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MC5R purchase    MBS904648 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the melanocortin 5 receptor (MC5R) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2 pg/ml-2000 pg/ml in biological research samples containing MC5R, with an estimated sensitivity of 7.8 pg/ml. The ELISA analytical biochemical technique of the MBS904648 kit is based on MC5R antibody-MC5R antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MC5R antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MC5R. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for MC5R elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MC5R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MC5R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MC5R is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MC5R bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of MC5R elisa kit    MC5R elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS904648. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MC5R. It may not necessarily be applicable to this product.
 NCBI GI #    729997
 NCBI GeneID    17203
 NCBI Accession #    P41149.1 [Other Products]
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 UniProt Primary Accession #    P41149 [Other Products]
 UniProt Related Accession #    P41149 [Other Products]
 Molecular Weight    36,953 Da
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 NCBI Official Full Name    Melanocortin receptor 5
 NCBI Official Synonym Full Names    melanocortin 5 receptor
 NCBI Official Symbol    Mc5r [Similar Products]
 NCBI Protein Information    melanocortin receptor 5; MC5-R
 UniProt Protein Name    Melanocortin receptor 5
 Protein Family    Melanocortin receptor
 UniProt Gene Name    Mc5r [Similar Products]
 UniProt Synonym Gene Names    MC5-R [Similar Products]
 UniProt Entry Name    MC5R_MOUSE
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 UniProt Comments for MC5R    MC5R: Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. This receptor is a possible mediator of the immunomodulation properties of melanocortins. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; signal transducer activity; hormone binding; melanocortin receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of cAMP biosynthetic process; signal transduction
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 Research Articles on MC5R    1. MC5r-dependent regulatory immunity increased CD11b(+)F4/80(+)Ly-6C(low)Ly-6G(+)CD39(+)CD73(+) APCs in the spleen of post-experimental autoimmune uveoretinitis mice.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with MC5R elisa kitOrgans/Tissues associated with MC5R elisa kit
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Peptide GPCRs Pathway antibodies  Peptide GPCRs Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >38 publications with MC5R and Brain
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