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MFAP5 elisa kit :: Mouse microfibrillar associated protein 5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS910817
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

microfibrillar associated protein 5 (MFAP5), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Microfibrillar-associated protein 5, MFAP5 ELISA Kit

 Product Synonym Names    Mouse Microfibrillar-associated protein 5 (MFAP5) ELISA kit; MAGP2; MP25; microfibril-associated glycoprotein 2; microfibril-associated glycoprotein-2; microfibrillar associated protein 5
 Product Gene Name   

MFAP5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS910817 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q9QZJ6
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse MFAP5. No significant cross-reactivity or interference between mouse MFAP5 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.156 ng/ml -10 ng/ml.
 Sensitivity    The minimum detectable dose of mouse MFAP5 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MFAP5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MFAP5 purchase    MBS910817 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the microfibrillar associated protein 5 (MFAP5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 ng/ml -10 ng/ml in biological research samples containing MFAP5, with an estimated sensitivity of The minimum detectable dose of mouse MFAP5 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS910817 kit is based on MFAP5 antibody-MFAP5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MFAP5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MFAP5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Research into extracellular matrix glycoproteins, such as microfibrillar associated protein 5 - 5 MFAP5 - have revealed novel roles in human disease. Mouse models require quantitative abilities for microfibrillar protein (MFAP), including MFAP5 ELISA for these glycoprotein studies. Manufactured by Promega or Roche Applied Science and distributed by MyBiosource, this MFAP5 ELISA kit product is ideal for cell samples detection of protein 5 MFAP5 for comparative cell proliferation assay. See assay system Promega for luciferase assay. Microfibrillar protein 5 - 5 MFAP5 ELISA is highly specific for microfibrillar protein 5 and will not cross react with MAGP 2, microfibrillar protein 4 - protein 4 MFAP4 - human microfibrillar protein or other microfibril glycoprotein products. Besides this detection kit, we offer sheep, rat, pig or monkey ELISA kits with the same high quality primary antibody and secondary antibodies. View kit microfibrillar protein to order for non diagnostic use only.
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Related Product Information for MFAP5 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MFAP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MFAP5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFAP5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFAP5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of MFAP5 elisa kit    MFAP5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS910817. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MFAP5. It may not necessarily be applicable to this product.
 NCBI GI #    7657329
 NCBI GeneID    50530
 NCBI Accession #    NP_056591.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_015776.2 [Other Products]
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 UniProt Primary Accession #    Q9QZJ6 [Other Products]
 UniProt Related Accession #    Q9QZJ6 [Other Products]
 Molecular Weight    18,538 Da
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 NCBI Official Full Name    microfibrillar-associated protein 5
 NCBI Official Synonym Full Names    microfibrillar associated protein 5
 NCBI Official Symbol    Mfap5 [Similar Products]
 NCBI Official Synonym Symbols   
MAGP-2
[Similar Products]
 NCBI Protein Information    microfibrillar-associated protein 5; MFAP-5; microfibril-associated glycoprotein 2
 UniProt Protein Name    Microfibrillar-associated protein 5
 UniProt Synonym Protein Names   
Microfibril-associated glycoprotein 2; MAGP-2
 Protein Family    Microfibrillar-associated protein
 UniProt Gene Name    Mfap5 [Similar Products]
 UniProt Synonym Gene Names    Magp2; MFAP-5; MAGP-2 [Similar Products]
 UniProt Entry Name    MFAP5_MOUSE
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 UniProt Comments for MFAP5    MFAP5: Component of the elastin-associated microfibrils. Belongs to the MFAP family.

Protein type: Secreted, signal peptide; Extracellular matrix; Secreted

Cellular Component: proteinaceous extracellular matrix; extracellular region; microfibril

Molecular Function: extracellular matrix constituent conferring elasticity

Biological Process: fibril organization and biogenesis
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 Research Articles on MFAP5    1. Loss of MAGP2 expression in vivo has pleiotropic effects.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MFAP5 elisa kitPathways associated with MFAP5 elisa kit
 Reference Product  PubMed Publications
 Fbln1 elisa kit  >1 publications with MFAP5 and Fbln1
 Fbn1 elisa kit  >1 publications with MFAP5 and Fbn1
 Products by Pathway  Pathway Diagram
 Elastic Fibre Formation Pathway antibodies  Elastic Fibre Formation Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Molecules Associated With Elastic Fibres Pathway antibodies  Molecules Associated With Elastic Fibres Pathway Diagram
Diseases associated with MFAP5 elisa kitOrgans/Tissues associated with MFAP5 elisa kit
 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >1 publications with MFAP5 and Insulin Resistance
 Infertility, Male Antibodies  >1 publications with MFAP5 and Infertility, Male
 Fibrosis Antibodies  >1 publications with MFAP5 and Fibrosis
 Inflammation Antibodies  >1 publications with MFAP5 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1 publications with MFAP5 and Lung
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