Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow MUC5B rightarrow Mouse MUC5B  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Aminoacyl tRNA Synthetase Complex Interacting Multifunctiona... ELISA Kit
 • erythropoietin (EPO) ELISA Kit
 • Glutathione S-Transferase Omega 1 Mutant (GSTO1) Recombinant Protein
 • LHX2 Antibody
 • Toxoplasma gondii p30 (SAG1) Recombinant Protein Recombinant Protein
 • CD300 molecule-like family member b (CD300LB) ELISA Kit
 • Thyroid Stimulating Hormone (TSH) intact & beta Antibody
 • deltex homolog 2 (DTX2) Antibody
 • Leutenizing Hormone Releasing Hormone (rHuLHRH; rHuGnRH) Recombinant Protein
 • Bone Alkaline Phosphatase (BALP) ELISA Kit
 • SLC2A4 Antibody
 • JIP3-Syd2-JSAP1 (MAPK8IP3) Antibody
 • Granulysin (GNLY) Antibody
 • BD-2 ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

MUC5B elisa kit :: Mouse mucin-5 subtype B, MUC5B ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705579
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

mucin-5 subtype B, MUC5B, ELISA Kit

★Popular Item★
 Also Known As   

Mouse mucin-5 subtype B, MUC5B ELISA Kit

 Product Synonym Names    Mouse mucin-5 subtype B; MUC5B ELISA Kit; mucin-5 subtype B; MUC5B
 Product Gene Name   

MUC5B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705579 Testing Data    Testing Data PDF
 OMIM    600770
 3D Structure    ModBase 3D Structure for Q9HC84
Section Bar
 Species Reactivity    Mouse
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse MUC5B . No significant cross-reactivity or interference between mouse MUC5B and analogues was observed.
Section Bar
 Samples    Serum, plasma, cell culture supernates and tissue homogenates
 Assay Type    Sandwich
 Detection Range    1.25 ng/ml -80 ng/ml.
 Sensitivity    The minimum detectable dose of mouse MUC5B is typically less than 0.31 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MUC5B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MUC5B purchase    MBS705579 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the mucin-5 subtype B, MUC5B, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.25 ng/ml -80 ng/ml in biological research samples containing MUC5B, with an estimated sensitivity of The minimum detectable dose of mouse MUC5B is typically less than 0.31 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS705579 kit is based on MUC5B antibody-MUC5B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MUC5B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MUC5B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates and tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for MUC5B elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MUC5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC5B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC5B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC5B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of MUC5B elisa kit    MUC5B elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS705579. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for MUC5B. It may not necessarily be applicable to this product.
 NCBI GI #    301172750
 NCBI GeneID    727897
 NCBI Accession #    NP_002449.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002458.2 [Other Products]
Section Bar
 UniProt Primary Accession #    Q9HC84 [Other Products]
 UniProt Secondary Accession #    O00447; O00573; O14985; O15494; O95291; O95451; Q14881; Q7M4S5; Q99552; Q9UE28 [Other Products]
 UniProt Related Accession #    Q9HC84 [Other Products]
 Molecular Weight    596,340 Da
Table BarTOPTable Bar
 NCBI Official Full Name    mucin-5B
 NCBI Official Synonym Full Names    mucin 5B, oligomeric mucus/gel-forming
 NCBI Official Symbol    MUC5B [Similar Products]
 NCBI Official Synonym Symbols   
MG1; MUC5; MUC9; MUC-5B
[Similar Products]
 NCBI Protein Information    mucin-5B; cervical mucin MUC5B; sublingual gland mucin; mucin 5, subtype B, tracheobronchial; high molecular weight salivary mucin MG1
 UniProt Protein Name    Mucin-5B
 UniProt Synonym Protein Names   
Cervical mucin; High molecular weight salivary mucin MG1; Mucin-5 subtype B, tracheobronchial; Sublingual gland mucin
 Protein Family    Mucin
 UniProt Gene Name    MUC5B [Similar Products]
 UniProt Synonym Gene Names    MUC5; MUC-5B [Similar Products]
 UniProt Entry Name    MUC5B_HUMAN
Section Bar
 NCBI Summary for MUC5B    This gene encodes a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases, including sinus mucosa of chronic rhinosinusitis (CRS), CRS with nasal polyposis, chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease, and it may be involved in the pathogenesis of these diseases. [provided by RefSeq, Jul 2010]
Section Bar
 UniProt Comments for MUC5B    Function: Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.
Table BarTOPTable Bar
 Research Articles on MUC5B    1. TSLP induces MUC5B expression via the ERK1/2 and p38 MAPK signaling pathway in human airway epithelial cells.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with MUC5B elisa kitDiseases associated with MUC5B elisa kit
 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 O-linked Glycosylation Pathway antibodies  O-linked Glycosylation Pathway Diagram
 O-linked Glycosylation Of Mucins Pathway antibodies  O-linked Glycosylation Of Mucins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Salivary Secretion Pathway antibodies  Salivary Secretion Pathway Diagram
 Salivary Secretion Pathway antibodies  Salivary Secretion Pathway Diagram
 Termination Of O-glycan Biosynthesis Pathway antibodies  Termination Of O-glycan Biosynthesis Pathway Diagram
 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >112 publications with MUC5B and Lung Diseases
 Inflammation Antibodies  >67 publications with MUC5B and Inflammation
 Immune System Diseases Antibodies  >34 publications with MUC5B and Immune System Diseases
 Idiopathic Pulmonary Fibrosis Antibodies  >31 publications with MUC5B and Idiopathic Pulmonary Fibrosis
 Hyperplasia Antibodies  >28 publications with MUC5B and Hyperplasia
 Necrosis Antibodies  >19 publications with MUC5B and Necrosis
 Lung Neoplasms Antibodies  >15 publications with MUC5B and Lung Neoplasms
 Respiration Disorders Antibodies  >11 publications with MUC5B and Respiration Disorders
 Ovarian Neoplasms Antibodies  >7 publications with MUC5B and Ovarian Neoplasms
 Ovarian Diseases Antibodies  >6 publications with MUC5B and Ovarian Diseases
Organs/Tissues associated with MUC5B elisa kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >105 publications with MUC5B and Lung
 Intestine Antibodies  >46 publications with MUC5B and Intestine
 Trachea Antibodies  >37 publications with MUC5B and Trachea
 Stomach Antibodies  >35 publications with MUC5B and Stomach
 Larynx Antibodies  >25 publications with MUC5B and Larynx
 Pancreas Antibodies  >12 publications with MUC5B and Pancreas
 Cervix Antibodies  >11 publications with MUC5B and Cervix
 Uterus Antibodies  >8 publications with MUC5B and Uterus
 Prostate Antibodies  >5 publications with MUC5B and Prostate
 Pharynx Antibodies  >2 publications with MUC5B and Pharynx
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions