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NAPSA elisa kit :: Mouse napsin A aspartic peptidase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS911879
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

napsin A aspartic peptidase (NAPSA), ELISA Kit

 Also Known As   

Mouse Napsin-A, NAPSA ELISA Kit

 Product Synonym Names    Mouse Napsin-A (NAPSA) ELISA kit; KAP; Kdap; NAP1; NAPA; SNAPA; napsin A; pronapsin A; napsin A aspartic peptidase
 Product Gene Name   

NAPSA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS911879 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for O09043
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse NAPSA. No significant cross-reactivity or interference between mouse NAPSA and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates
 Assay Type    Sandwich
 Detection Range    6.25 pg/ml -400 pg/ml.
 Sensitivity    The minimum detectable dose of mouse NAPSA is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NAPSA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NAPSA purchase    MBS911879 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the napsin A aspartic peptidase (NAPSA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 pg/ml -400 pg/ml in biological research samples containing NAPSA, with an estimated sensitivity of The minimum detectable dose of mouse NAPSA is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS911879 kit is based on NAPSA antibody-NAPSA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NAPSA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NAPSA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for NAPSA elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NAPSA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAPSA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAPSA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAPSA bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of NAPSA elisa kit    NAPSA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS911879. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NAPSA. It may not necessarily be applicable to this product.
 NCBI GI #    6680552
 NCBI GeneID    16541
 NCBI Accession #    NP_032463.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_008437.1 [Other Products]
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 UniProt Primary Accession #    O09043 [Other Products]
 UniProt Related Accession #    O09043 [Other Products]
 Molecular Weight    45,544 Da
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 NCBI Official Full Name    napsin-A
 NCBI Official Synonym Full Names    napsin A aspartic peptidase
 NCBI Official Symbol    Napsa [Similar Products]
 NCBI Official Synonym Symbols   
KAP; Kdap; NAP1; SNAPA; pronapsin
[Similar Products]
 NCBI Protein Information    napsin-A; KDAP-1; kidney-derived aspartic protease-like protein
 UniProt Protein Name    Napsin-A
 UniProt Synonym Protein Names   
KDAP-1; Kidney-derived aspartic protease-like protein; KAP
 Protein Family    Napsin
 UniProt Gene Name    Napsa [Similar Products]
 UniProt Synonym Gene Names    Kdap; Nap; KAP [Similar Products]
 UniProt Entry Name    NAPSA_MOUSE
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 UniProt Comments for NAPSA    NAPSA: May be involved in processing of pneumocyte surfactant precursors. Belongs to the peptidase A1 family.

Protein type: Secreted, signal peptide; Secreted; EC 3.4.23.-; Protease

Cellular Component: extracellular space; lysosome; extracellular region

Molecular Function: peptidase activity; protein binding; hydrolase activity; aspartic endopeptidase activity, intramembrane cleaving; endopeptidase activity; aspartic-type endopeptidase activity

Biological Process: membrane protein proteolysis; proteolysis; surfactant homeostasis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with NAPSA elisa kitDiseases associated with NAPSA elisa kit
 Products by Pathway  Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Disease Name  Pubmed Publications
 Skin Diseases Antibodies  >3 publications with NAPSA and Skin Diseases
 Fibrosis Antibodies  >2 publications with NAPSA and Fibrosis
 Gastrointestinal Diseases Antibodies  >2 publications with NAPSA and Gastrointestinal Diseases
 Pleural Effusion Antibodies  >2 publications with NAPSA and Pleural Effusion
 Hyperplasia Antibodies  >1 publications with NAPSA and Hyperplasia
 Mental Disorders Antibodies  >1 publications with NAPSA and Mental Disorders
 Skin Neoplasms Antibodies  >1 publications with NAPSA and Skin Neoplasms
 Pain Antibodies  >1 publications with NAPSA and Pain
 Disease Models, Animal Antibodies  >1 publications with NAPSA and Disease Models, Animal
 Acute Lung Injury Antibodies  >1 publications with NAPSA and Acute Lung Injury
Organs/Tissues associated with NAPSA elisa kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >31 publications with NAPSA and Lung
 Thyroid Antibodies  >12 publications with NAPSA and Thyroid
 Kidney Antibodies  >12 publications with NAPSA and Kidney
 Blood Antibodies  >5 publications with NAPSA and Blood
 Pancreas Antibodies  >2 publications with NAPSA and Pancreas
 Lymph Node Antibodies  >2 publications with NAPSA and Lymph Node
 Skin Antibodies  >2 publications with NAPSA and Skin
 Liver Antibodies  >2 publications with NAPSA and Liver
 Spleen Antibodies  >2 publications with NAPSA and Spleen
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