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NPFFR2 elisa kit :: Mouse neuropeptide FF receptor 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS902984
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

neuropeptide FF receptor 2 (NPFFR2), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Neuropeptide FF receptor 2, NPFFR2 ELISA Kit

 Product Synonym Names    Mouse Neuropeptide FF receptor 2 (NPFFR2) ELISA kit; GPR74; HLWAR77; NPFF2; NPGPR; G protein-coupled receptor 74; OTTHUMP00000160368; neuropeptide FF 2; neuropeptide FF receptor 2
 Product Gene Name   

NPFFR2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS902984 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q924H0
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse NPFFR2. No significant cross-reactivity or interference between mouse NPFFR2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml.
 Sensitivity    The minimum detectable dose of mouse NPFFR2 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NPFFR2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NPFFR2 purchase    MBS902984 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the neuropeptide FF receptor 2 (NPFFR2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing NPFFR2, with an estimated sensitivity of The minimum detectable dose of mouse NPFFR2 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS902984 kit is based on NPFFR2 antibody-NPFFR2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NPFFR2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NPFFR2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NPFFR2 elisa kit

   Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NPFFR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NPFFR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPFFR2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPFFR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of NPFFR2 elisa kit    NPFFR2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS902984. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NPFFR2. It may not necessarily be applicable to this product.
 NCBI GI #    31981539
 NCBI GeneID    104443
 NCBI Accession #    NP_573455.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_133192.3 [Other Products]
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 UniProt Primary Accession #    Q924H0 [Other Products]
 UniProt Secondary Accession #    Q8BKR6 [Other Products]
 UniProt Related Accession #    Q924H0 [Other Products]
 Molecular Weight    47,449 Da
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 NCBI Official Full Name    neuropeptide FF receptor 2
 NCBI Official Synonym Full Names    neuropeptide FF receptor 2
 NCBI Official Symbol    Npffr2 [Similar Products]
 NCBI Official Synonym Symbols   
HG31; Gpr74; NPFF2
[Similar Products]
 NCBI Protein Information    neuropeptide FF receptor 2; neuropeptide NPFF receptor; G protein-coupled receptor 74; G-protein coupled receptor 74
 UniProt Protein Name    Neuropeptide FF receptor 2
 UniProt Synonym Protein Names   
G-protein coupled receptor 74; Neuropeptide NPFF receptor
 Protein Family    Neuropeptide FF receptor
 UniProt Gene Name    Npffr2 [Similar Products]
 UniProt Synonym Gene Names    Gpr74; Npff2 [Similar Products]
 UniProt Entry Name    NPFF2_MOUSE
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 UniProt Comments for NPFFR2    NPFFR2: Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane; actin cytoskeleton

Molecular Function: opioid receptor binding; G-protein coupled receptor activity; neuropeptide receptor activity; signal transducer activity; peptide binding

Biological Process: regulation of adenylate cyclase activity; G-protein coupled receptor protein signaling pathway; synaptic transmission; neuropeptide signaling pathway; regulation of MAPKKK cascade; signal transduction; cellular response to hormone stimulus
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 Research Articles on NPFFR2    1. Data showed that 33% of GnRH neurons and 9-16% of rostral periventricular kisspeptin neurons expressed Gpr147, whereas Gpr74 was not expressed in either population.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with NPFFR2 elisa kitDiseases associated with NPFFR2 elisa kit
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Orexin And Neuropeptides FF And QRFP Bind To Their Respective Receptors Pathway antibodies  Orexin And Neuropeptides FF And QRFP Bind To Their Respective Receptors Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
 Disease Name  Pubmed Publications
 Hydrocephalus Antibodies  >1 publications with NPFFR2 and Hydrocephalus
 Central Nervous System Neoplasms Antibodies  >1 publications with NPFFR2 and Central Nervous System Neoplasms
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