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NOS3 elisa kit :: Mouse nitric oxide synthase 3 (endothelial cell) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700721
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

nitric oxide synthase 3 (endothelial cell) (NOS3), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Endothelial nitric oxide synthase, eNOS ELISA Kit

 Product Synonym Names    Mouse Endothelial nitric oxide synthase; eNOS ELISA Kit; ECNOS; eNOS; nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell)
 Product Gene Name   

NOS3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700721 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P70313
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse NOS3. No significant cross-reactivity or interference between Mouse NOS3 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Detection Range    0.312 IU/ml-20 IU/ml
 Sensitivity    0.078 IU/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NOS3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NOS3 purchase    MBS700721 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nitric oxide synthase 3 (endothelial cell) (NOS3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 IU/ml-20 IU/ml in biological research samples containing NOS3, with an estimated sensitivity of 0.078 IU/ml. The ELISA analytical biochemical technique of the MBS700721 kit is based on NOS3 antibody-NOS3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NOS3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NOS3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NOS3 elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to eNOS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for eNOS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain eNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of eNOS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of NOS3 elisa kit    NOS3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700721. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NOS3. It may not necessarily be applicable to this product.
 NCBI GI #    161086988
 NCBI GeneID    18127
 NCBI Accession #    NP_032739.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_008713.4 [Other Products]
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 UniProt Primary Accession #    P70313 [Other Products]
 UniProt Secondary Accession #    O55056; Q7TSV7 [Other Products]
 UniProt Related Accession #    P70313 [Other Products]
 Molecular Weight    132,916 Da
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 NCBI Official Full Name    nitric oxide synthase, endothelial
 NCBI Official Synonym Full Names    nitric oxide synthase 3, endothelial cell
 NCBI Official Symbol    Nos3 [Similar Products]
 NCBI Official Synonym Symbols   
eNOS; Nos-3; ecNOS; 2310065A03Rik
[Similar Products]
 NCBI Protein Information    nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; endothelial nitric oxide synthase
 UniProt Protein Name    Nitric oxide synthase, endothelial
 UniProt Synonym Protein Names   
Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
 Protein Family    Nitric oxide synthase
 UniProt Gene Name    Nos3 [Similar Products]
 UniProt Synonym Gene Names    Ecnos; cNOS; eNOS; NOSIII [Similar Products]
 UniProt Entry Name    NOS3_MOUSE
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 UniProt Comments for NOS3    Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.1 PublicationManual assertion based on experiment in:Ref.3

Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor: Heme group.

Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN
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 Research Articles on NOS3    1. In diabetic mice vascular endothelium eNOS phosphorylation was impaired.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NOS3 elisa kitPathways associated with NOS3 elisa kit
 Reference Product  PubMed Publications
 Ace elisa kit  >150 publications with NOS3 and Ace
 Akt1 elisa kit  >123 publications with NOS3 and Akt1
 Vegfa elisa kit  >70 publications with NOS3 and Vegfa
 Cav1 elisa kit  >69 publications with NOS3 and Cav1
 Agt elisa kit  >52 publications with NOS3 and Agt
 Cav3 elisa kit  >11 publications with NOS3 and Cav3
 Nosip elisa kit  >5 publications with NOS3 and Nosip
 Nostrin elisa kit  >4 publications with NOS3 and Nostrin
 Hsp90aa1 elisa kit  >1 publications with NOS3 and Hsp90aa1
 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Estrogen Signaling Pathway antibodies  Estrogen Signaling Pathway Diagram
 Estrogen Signaling Pathway antibodies  Estrogen Signaling Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
Diseases associated with NOS3 elisa kitOrgans/Tissues associated with NOS3 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >2093 publications with NOS3 and Cardiovascular Diseases
 Hypertension Antibodies  >1035 publications with NOS3 and Hypertension
 Myocardial Ischemia Antibodies  >564 publications with NOS3 and Myocardial Ischemia
 Ischemia Antibodies  >548 publications with NOS3 and Ischemia
 Atherosclerosis Antibodies  >453 publications with NOS3 and Atherosclerosis
 Reperfusion Injury Antibodies  >279 publications with NOS3 and Reperfusion Injury
 Myocardial Infarction Antibodies  >251 publications with NOS3 and Myocardial Infarction
 Coronary Artery Disease Antibodies  >249 publications with NOS3 and Coronary Artery Disease
 Cerebral Infarction Antibodies  >200 publications with NOS3 and Cerebral Infarction
 Diabetes Mellitus, Experimental Antibodies  >193 publications with NOS3 and Diabetes Mellitus, Experimental
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >1238 publications with NOS3 and Heart
 Brain Antibodies  >440 publications with NOS3 and Brain
 Lung Antibodies  >379 publications with NOS3 and Lung
 Embryonic Tissue Antibodies  >210 publications with NOS3 and Embryonic Tissue
 Intestine Antibodies  >83 publications with NOS3 and Intestine
 Eye Antibodies  >50 publications with NOS3 and Eye
 Testis Antibodies  >27 publications with NOS3 and Testis
 Spleen Antibodies  >19 publications with NOS3 and Spleen
 Inner Ear Antibodies  >10 publications with NOS3 and Inner Ear
 Mammary Gland Antibodies  >4 publications with NOS3 and Mammary Gland
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