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NFE2L2 elisa kit :: Mouse nuclear factor (erythroid-derived 2) -like 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705641
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
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 Product Name   

nuclear factor (erythroid-derived 2) -like 2 (NFE2L2), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Nuclear factor erythroid 2-related factor 2, Nrf2 ELISA Kit

 Product Synonym Names    Mouse Nuclear factor erythroid 2-related factor 2 (Nrf2) ELISA Kit; NRF2; NF-E2-related factor 2; nuclear factor erythroid 2-like 2; nuclear factor (erythroid-derived 2) -like 2
 Product Gene Name   

NFE2L2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705641 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q60795
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse NFE2L2. No significant cross-reactivity or interference between Mouse NFE2L2 and analogues was observed.
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 Samples    Serum, plasma and cell culture supernates
 Detection Range    0.312 ng/ml-20 ng/ml
 Sensitivity    0.078 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Transcription/Transcription regulation
 Protein Biological Process 3    Transcription
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NFE2L2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NFE2L2 purchase    MBS705641 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nuclear factor (erythroid-derived 2) -like 2 (NFE2L2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml-20 ng/ml in biological research samples containing NFE2L2, with an estimated sensitivity of 0.078 ng/ml. The ELISA analytical biochemical technique of the MBS705641 kit is based on NFE2L2 antibody-NFE2L2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NFE2L2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NFE2L2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and cell culture supernates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NFE2L2 elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear factor erythroid 2-related factor 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Nuclear factor erythroid 2-related factor 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Nuclear factor erythroid 2-related factor 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Nuclear factor erythroid 2-related factor 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of NFE2L2 elisa kit    NFE2L2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705641. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NFE2L2. It may not necessarily be applicable to this product.
 NCBI GI #    6754832
 NCBI GeneID    18024
 NCBI Accession #    NP_035032.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_010902.3 [Other Products]
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 UniProt Primary Accession #    Q60795 [Other Products]
 UniProt Secondary Accession #    Q3UQK0 [Other Products]
 UniProt Related Accession #    Q60795 [Other Products]
 Molecular Weight    66,901 Da
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 NCBI Official Full Name    nuclear factor erythroid 2-related factor 2
 NCBI Official Synonym Full Names    nuclear factor, erythroid derived 2, like 2
 NCBI Official Symbol    Nfe2l2 [Similar Products]
 NCBI Official Synonym Symbols   
Nrf2; AI194320
[Similar Products]
 NCBI Protein Information    nuclear factor erythroid 2-related factor 2; NFE2-related factor 2; NF-E2-related factor 2
 UniProt Protein Name    Nuclear factor erythroid 2-related factor 2
 UniProt Synonym Protein Names   
Nuclear factor, erythroid derived 2, like 2
 Protein Family    Nuclear factor erythroid 2-related factor
 UniProt Gene Name    Nfe2l2 [Similar Products]
 UniProt Synonym Gene Names    Nrf-2; Nrf2; NF-E2-related factor 2; NFE2-related factor 2 [Similar Products]
 UniProt Entry Name    NF2L2_MOUSE
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 NCBI Summary for NFE2L2    This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. [provided by RefSeq, Sep 2015]
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 UniProt Comments for NFE2L2    Function: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.1 PublicationManual assertion based on experiment in:Ref.6
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 Research Articles on NFE2L2    1. show that Nrf2 protects SM from TWEAK-induced cell death in vitro and that Nrf2-deficient mice therefore have poorer muscle regeneration
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NFE2L2 elisa kitPathways associated with NFE2L2 elisa kit
 Reference Product  PubMed Publications
 KEAP1 elisa kit  >233 publications with NFE2L2 and KEAP1
 JUN elisa kit  >47 publications with NFE2L2 and JUN
 CASP3 elisa kit  >33 publications with NFE2L2 and CASP3
 MAFK elisa kit  >18 publications with NFE2L2 and MAFK
 NFE2 elisa kit  >17 publications with NFE2L2 and NFE2
 PPARG elisa kit  >15 publications with NFE2L2 and PPARG
 MAFG elisa kit  >12 publications with NFE2L2 and MAFG
 JUND elisa kit  >8 publications with NFE2L2 and JUND
 ATF4 elisa kit  >7 publications with NFE2L2 and ATF4
 PMF1 elisa kit  >6 publications with NFE2L2 and PMF1
 Products by Pathway  Pathway Diagram
 Keap1-Nrf2 Pathway antibodies  Keap1-Nrf2 Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Selenium Metabolism/Selenoproteins Pathway antibodies  Selenium Metabolism/Selenoproteins Pathway Diagram
Diseases associated with NFE2L2 elisa kitOrgans/Tissues associated with NFE2L2 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >523 publications with NFE2L2 and Inflammation
 Disease Models, Animal Antibodies  >294 publications with NFE2L2 and Disease Models, Animal
 Nervous System Diseases Antibodies  >294 publications with NFE2L2 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >250 publications with NFE2L2 and Cardiovascular Diseases
 Necrosis Antibodies  >233 publications with NFE2L2 and Necrosis
 Liver Diseases Antibodies  >229 publications with NFE2L2 and Liver Diseases
 Liver Neoplasms Antibodies  >99 publications with NFE2L2 and Liver Neoplasms
 Kidney Diseases Antibodies  >97 publications with NFE2L2 and Kidney Diseases
 Fibrosis Antibodies  >95 publications with NFE2L2 and Fibrosis
 Lung Neoplasms Antibodies  >92 publications with NFE2L2 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >169 publications with NFE2L2 and Liver
 Blood Antibodies  >132 publications with NFE2L2 and Blood
 Lung Antibodies  >123 publications with NFE2L2 and Lung
 Brain Antibodies  >82 publications with NFE2L2 and Brain
 Vascular Antibodies  >79 publications with NFE2L2 and Vascular
 Muscle Antibodies  >44 publications with NFE2L2 and Muscle
 Kidney Antibodies  >41 publications with NFE2L2 and Kidney
 Heart Antibodies  >36 publications with NFE2L2 and Heart
 Skin Antibodies  >30 publications with NFE2L2 and Skin
 Eye Antibodies  >29 publications with NFE2L2 and Eye
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