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NRF1 elisa kit :: Mouse nuclear respiratory factor 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS915302
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

nuclear respiratory factor 1 (NRF1), ELISA Kit

 Also Known As   

Mouse Nuclear respiratory factor 1, NRF1 ELISA Kit

 Product Synonym Names    Mouse Nuclear respiratory factor 1 (NRF1) ELISA kit; ALPHA-PAL; OTTHUMP00000184912; alpha palindromic-binding protein; nuclear respiratory factor 1
 Product Gene Name   

NRF1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS915302 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q9WU00
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse NRF1. No significant cross-reactivity or interference between mouse NRF1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    15.6 pg/ml -1000 pg/ml.
 Sensitivity    The minimum detectable dose of mouse NRF1 is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NRF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NRF1 purchase    MBS915302 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nuclear respiratory factor 1 (NRF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml -1000 pg/ml in biological research samples containing NRF1, with an estimated sensitivity of The minimum detectable dose of mouse NRF1 is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS915302 kit is based on NRF1 antibody-NRF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NRF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NRF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for NRF1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NRF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NRF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of NRF1 elisa kit    NRF1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS915302. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NRF1. It may not necessarily be applicable to this product.
 NCBI GI #    255982544
 NCBI GeneID    18181
 NCBI Accession #    NP_001157698.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001164226.1 [Other Products]
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 UniProt Primary Accession #    Q9WU00 [Other Products]
 UniProt Secondary Accession #    Q9CXE4 [Other Products]
 UniProt Related Accession #    Q9WU00 [Other Products]
 Molecular Weight    53,571 Da
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 NCBI Official Full Name    nuclear respiratory factor 1 isoform a
 NCBI Official Synonym Full Names    nuclear respiratory factor 1
 NCBI Official Symbol    Nrf1 [Similar Products]
 NCBI Official Synonym Symbols   
C87038; D6Ertd415e
[Similar Products]
 NCBI Protein Information    nuclear respiratory factor 1; NRF-1; alpha-pal; alpha palindromic-binding protein
 UniProt Protein Name    Nuclear respiratory factor 1
 UniProt Synonym Protein Names   
Alpha palindromic-binding protein; Alpha-pal
 Protein Family    Nuclear respiratory factor
 UniProt Gene Name    Nrf1 [Similar Products]
 UniProt Synonym Gene Names    NRF-1; Alpha-pal [Similar Products]
 UniProt Entry Name    NRF1_MOUSE
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 UniProt Comments for NRF1    NRF1: Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. Belongs to the NRF1/Ewg family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Cellular Component: transcription factor complex; cytoplasm; nucleus

Molecular Function: DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; response to electrical stimulus
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 Research Articles on NRF1    1. Data indicate both Nrf1a and Nrf1b isoforms transcripts were detected in different mouse and human cell lines, and in various mouse tissues.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with NRF1 elisa kitDiseases associated with NRF1 elisa kit
 Products by Pathway  Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Mitochondrial Gene Expression Pathway antibodies  Mitochondrial Gene Expression Pathway Diagram
 Disease Name  Pubmed Publications
 Mitochondrial Diseases Antibodies  >19 publications with NRF1 and Mitochondrial Diseases
 Nervous System Diseases Antibodies  >17 publications with NRF1 and Nervous System Diseases
 Insulin Resistance Antibodies  >16 publications with NRF1 and Insulin Resistance
 Liver Diseases Antibodies  >14 publications with NRF1 and Liver Diseases
 Diabetes Mellitus, Type 2 Antibodies  >14 publications with NRF1 and Diabetes Mellitus, Type 2
 Inflammation Antibodies  >11 publications with NRF1 and Inflammation
 Fatty Liver Antibodies  >10 publications with NRF1 and Fatty Liver
 Necrosis Antibodies  >10 publications with NRF1 and Necrosis
 Cardiovascular Diseases Antibodies  >9 publications with NRF1 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >9 publications with NRF1 and Disease Models, Animal
Organs/Tissues associated with NRF1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >60 publications with NRF1 and Muscle
 Liver Antibodies  >33 publications with NRF1 and Liver
 Heart Antibodies  >17 publications with NRF1 and Heart
 Brain Antibodies  >15 publications with NRF1 and Brain
 Lung Antibodies  >11 publications with NRF1 and Lung
 Skin Antibodies  >8 publications with NRF1 and Skin
 Kidney Antibodies  >8 publications with NRF1 and Kidney
 Embryonic Tissue Antibodies  >8 publications with NRF1 and Embryonic Tissue
 Eye Antibodies  >6 publications with NRF1 and Eye
 Testis Antibodies  >5 publications with NRF1 and Testis
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