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PTHLH elisa kit :: Mouse parathyroid hormone-like hormone ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703635
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $575 +1 FREE 8GB USB
96-Strip-Wells  /  $835 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
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 Product Name   

parathyroid hormone-like hormone (PTHLH), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Parathyroid Hormone Related Protein, PTHrP ELISA Kit

 Product Synonym Names    Mouse Parathyroid Hormone Related Protein (PTHrP) ELISA Kit; HHM; MGC14611; PLP; PTHR; PTHRP; PTH-related protein; humoral hypercalcemia of malignancy; osteostatin; parathyroid hormone-like protein; parathyroid hormone-like related protein; parathyroid hormone-rel; parathyroid hormone-like hormone
 Product Gene Name   

PTHLH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703635 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P22858
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse PTHLH. No significant cross-reactivity or interference between Mouse PTHLH and analogues was observed.
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 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PTHLH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PTHLH purchase    MBS703635 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the parathyroid hormone-like hormone (PTHLH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PTHLH. The ELISA analytical biochemical technique of the MBS703635 kit is based on PTHLH antibody-PTHLH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PTHLH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PTHLH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PTHLH elisa kit

   Introduction: Parathyroid hormone-related protein (or PTHrP) is a protein member of the parathyroid hormone family. It is occasionally secreted by cancer cells (breast cancer, certain types of lung cancer including squamous cell carcinoma). However, it also has normal functions. PTHrP acts as an endocrine, autocrine, paracrine, and intracrine hormone. It regulates endochondral bone development by maintaining the endochondral growth plate at a constant width. It also regulates epithelial-mesenchymal interactions during the formation of the mammary glands. PTHrP is critical in the intraosseous phase of tooth eruption where it acts as a signalling molecule to stimulate local bone resorption. Without PTHrP, the bony crypt surrounding the tooth follicle will not resorb, and therefore the tooth will not erupt. In the context of tooth eruption, PTHrP is secreted by the cells of the Reduced Enamel Epithelium. Besides, PTHrP is necessary for maintenance of the mammary bud. Loss of PTHrP or its receptor causes the mammary bud cell fate to change back into epidermis. In lactation, it may regulate in conjunction with the calcium sensing receptor the mobilization and transfer of calcium to the milk, as well as placental transfer of calcium. PTHrP is related in function to the "normal" parathyroid hormone. When a tumor secretes PTHrP, this can lead to hypercalcemia. As this is sometimes the first sign of the malignancy, hypercalcemia caused by PTHrP is considered a paraneoplastic phenomenon. PTHrP shares the same N-terminal end as parathyroid hormone and therefore it can bind to the same receptor, the Type I PTH receptor (PTHR1).

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTHrP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for PTHrP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PTHrP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PTHrP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PTHLH elisa kit    PTHLH elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703635. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PTHLH. It may not necessarily be applicable to this product.
 NCBI GI #    131543
 NCBI GeneID    19227
 NCBI Accession #    P22858.1 [Other Products]
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 UniProt Primary Accession #    P22858 [Other Products]
 UniProt Related Accession #    P22858 [Other Products]
 Molecular Weight    20,100 Da
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 NCBI Official Full Name    Parathyroid hormone-related protein
 NCBI Official Synonym Full Names    parathyroid hormone-like peptide
 NCBI Official Symbol    Pthlh [Similar Products]
 NCBI Official Synonym Symbols   
Pthrp; PTH-like
[Similar Products]
 NCBI Protein Information    parathyroid hormone-related protein; PLP; PTH-rP; PTH-related peptide; parathyroid hormone-like hormone; parathyroid hormone-like protein; parathyroid hormone-related peptide
 UniProt Protein Name    Parathyroid hormone-related protein
 UniProt Synonym Protein Names   
Parathyroid hormone-like protein; PLP
 Protein Family    Parathyroid hormone-related protein
 UniProt Gene Name    Pthlh [Similar Products]
 UniProt Synonym Gene Names    Pthrp; PTH-rP; PTHrP; PLP [Similar Products]
 UniProt Entry Name    PTHR_MOUSE
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 NCBI Summary for PTHLH    This gene encodes a member of the parathyroid family of hormones that possesses distinct paracrine and intracrine signaling roles such as regulation of circulating calcium, transplacental calcium transport, osteoclast inhibition, renal bicarbonate excretion and regulation of apoptosis. The encoded protein undergoes proteolytic processing to generate multiple active peptides with distinct signaling functions. The homozygous deletion of this gene leads to death shortly after birth with a chondrodystrophic phenotype characterized by premature chondrocyte differentiation and accelerated bone formation. [provided by RefSeq, Jul 2015]
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 UniProt Comments for PTHLH    Function: Neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Required for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Upregulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath.2 PublicationsManual assertion based on experiment in:Ref.3
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 Research Articles on PTHLH    1. Data suggest PTHrP haploinsufficiency impairs fracture repair by down-regulation of (1) cartilaginous and bony callus formation, (2) osteoblastic gene expression, (3) endochondral/osteoblastic bone formation, and (4) osteoclastic bone resorption.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PTHLH elisa kitPathways associated with PTHLH elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with PTHLH and FLNA
 Products by Pathway  Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
Organs/Tissues associated with PTHLH elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with PTHLH and Bone
 Connective Tissue Antibodies  >4 publications with PTHLH and Connective Tissue
 Heart Antibodies  >3 publications with PTHLH and Heart
 Muscle Antibodies  >3 publications with PTHLH and Muscle
 Brain Antibodies  >3 publications with PTHLH and Brain
 Vascular Antibodies  >2 publications with PTHLH and Vascular
 Embryonic Tissue Antibodies  >2 publications with PTHLH and Embryonic Tissue
 Ear Antibodies  >1 publications with PTHLH and Ear
 Kidney Antibodies  >1 publications with PTHLH and Kidney
 Umbilical Cord Antibodies  >1 publications with PTHLH and Umbilical Cord
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