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PRDX1 elisa kit :: Mouse peroxiredoxin 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS907485
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

peroxiredoxin 1 (PRDX1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Peroxiredoxin-1, PRDX1 ELISA Kit

 Product Synonym Names    Mouse Peroxiredoxin-1 (PRDX1) ELISA kit; MSP23; NKEFA; PAG; PAGA; PAGB; PRX1; PRXI; TDPX2; natural killer-enhancing factor A; proliferation-associated gene A; thioredoxin-dependent peroxide reductase 2; peroxiredoxin 1
 Product Gene Name   

PRDX1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS907485 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P35700
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse PRDX1. No significant cross-reactivity or interference between mouse PRDX1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.312 ng/ml -20 ng/ml.
 Sensitivity    The minimum detectable dose of mouse PRDX1 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PRDX1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PRDX1 purchase    MBS907485 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the peroxiredoxin 1 (PRDX1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml -20 ng/ml in biological research samples containing PRDX1, with an estimated sensitivity of The minimum detectable dose of mouse PRDX1 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS907485 kit is based on PRDX1 antibody-PRDX1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRDX1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRDX1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PRDX1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRDX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRDX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of PRDX1 elisa kit    PRDX1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS907485. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PRDX1. It may not necessarily be applicable to this product.
 NCBI GI #    6754976
 NCBI GeneID    18477
 NCBI Accession #    NP_035164.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_011034.4 [Other Products]
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 UniProt Primary Accession #    P35700 [Other Products]
 UniProt Secondary Accession #    Q3UBV4; Q9CWI2 [Other Products]
 UniProt Related Accession #    P35700 [Other Products]
 Molecular Weight    22,176 Da
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 NCBI Official Full Name    peroxiredoxin-1
 NCBI Official Synonym Full Names    peroxiredoxin 1
 NCBI Official Symbol    Prdx1 [Similar Products]
 NCBI Official Synonym Symbols   
PAG; OSF3; Paga; PrxI; TDX2; TPxA; prx1; MSP23; NkefA; OSF-3; PrdxI; Tdpx2
[Similar Products]
 NCBI Protein Information    peroxiredoxin-1; thioredoxin peroxidase 2; osteoblast specific factor 3; osteoblast-specific factor 3; macrophage 23 Kd stress protein; macrophage 23kDa stress protein; macrophase stress protein 22kDa; macrophase stress protein 23 kd; proliferation-associated gene A; macrophage 23 kDa stress protein; Trx dependent peroxide reductase 2; thioredoxin dependent peroxide reductase 2; thioredoxin-dependent peroxide reductase 2
 UniProt Protein Name    Peroxiredoxin-1
 UniProt Synonym Protein Names   
Macrophage 23 kDa stress protein; Osteoblast-specific factor 3; OSF-3; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2
 Protein Family    Peroxiredoxin
 UniProt Gene Name    Prdx1 [Similar Products]
 UniProt Synonym Gene Names    Msp23; Paga; Tdpx2; OSF-3 [Similar Products]
 UniProt Entry Name    PRDX1_MOUSE
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 UniProt Comments for PRDX1    PRDX1: a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). May have a proliferative effect and play a role in cancer development or progression.

Protein type: Oxidoreductase; Nuclear receptor co-regulator; EC 1.11.1.15

Cellular Component: peroxisomal matrix; extracellular space; mitochondrion; mitochondrial matrix; cytoplasm; nucleolus; cytosol; nucleus; myelin sheath

Molecular Function: antioxidant activity; identical protein binding; protein binding; peroxidase activity; protein homodimerization activity; peroxiredoxin activity; thioredoxin peroxidase activity; heme binding; oxidoreductase activity

Biological Process: regulation of stress-activated MAPK cascade; erythrocyte homeostasis; cell proliferation; response to reactive oxygen species; removal of superoxide radicals; natural killer cell mediated cytotoxicity; hydrogen peroxide catabolic process; response to oxidative stress; regulation of NF-kappaB import into nucleus
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 Research Articles on PRDX1    1. Prx I is an antioxidant that is up-regulated in a reactive oxygen species/p38 MAPK-dependent manner and governs the progression of neuroinflammation by suppressing microglial activation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PRDX1 elisa kitDiseases associated with PRDX1 elisa kit
 Products by Pathway  Pathway Diagram
 Peroxisome Pathway antibodies  Peroxisome Pathway Diagram
 Peroxisome Pathway antibodies  Peroxisome Pathway Diagram
 Phosphate Acquisition II Pathway antibodies  Phosphate Acquisition II Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >46 publications with PRDX1 and Neoplasms
 Carcinoma Antibodies  >20 publications with PRDX1 and Carcinoma
 Adenocarcinoma Antibodies  >15 publications with PRDX1 and Adenocarcinoma
 Drug Toxicity Antibodies  >15 publications with PRDX1 and Drug Toxicity
 Lung Diseases Antibodies  >14 publications with PRDX1 and Lung Diseases
 Lung Neoplasms Antibodies  >11 publications with PRDX1 and Lung Neoplasms
 Liver Diseases Antibodies  >9 publications with PRDX1 and Liver Diseases
 Inflammation Antibodies  >8 publications with PRDX1 and Inflammation
 Nervous System Diseases Antibodies  >7 publications with PRDX1 and Nervous System Diseases
 Disease Models, Animal Antibodies  >7 publications with PRDX1 and Disease Models, Animal
Organs/Tissues associated with PRDX1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >23 publications with PRDX1 and Blood
 Lung Antibodies  >18 publications with PRDX1 and Lung
 Liver Antibodies  >18 publications with PRDX1 and Liver
 Brain Antibodies  >10 publications with PRDX1 and Brain
 Muscle Antibodies  >9 publications with PRDX1 and Muscle
 Kidney Antibodies  >5 publications with PRDX1 and Kidney
 Heart Antibodies  >5 publications with PRDX1 and Heart
 Prostate Antibodies  >4 publications with PRDX1 and Prostate
 Embryonic Tissue Antibodies  >4 publications with PRDX1 and Embryonic Tissue
 Spinal Cord Antibodies  >3 publications with PRDX1 and Spinal Cord
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