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PLA2G4A elisa kit :: Mouse phospholipase A2, group IVA (cytosolic, calcium-dependent) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705411
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $575 +1 FREE 8GB USB
96-Strip-Wells  /  $835 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A), ELISA Kit

★Popular Item★
 Also Known As   

Mouse cytosolic phospholipase A2, cPLA2 ELISA Kit

 Product Synonym Names    Mouse cytosolic phospholipase A2; cPLA2 ELISA Kit; MGC126350; PLA2G4; cPLA2-alpha; calcium-dependent phospholipid-binding protein; cytosolic phospholipase A2; group IVA; lysophospholipase; phosphatidylcholine 2-acylhydrolase; phospholipase A2; group IVA (cytosolic; calcium-dependent)
 Product Gene Name   

PLA2G4A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705411 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P47713
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse cPLA2. No significant cross-reactivity or interference between mouse cPLA2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    62.5 pg/ml -4000 pg/ml.
 Sensitivity    The minimum detectable dose of mouse cPLA2 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PLA2G4A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PLA2G4A purchase    MBS705411 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 pg/ml -4000 pg/ml in biological research samples containing PLA2G4A, with an estimated sensitivity of The minimum detectable dose of mouse cPLA2 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS705411 kit is based on PLA2G4A antibody-PLA2G4A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLA2G4A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLA2G4A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PLA2G4A elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for cPLA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cPLA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cPLA2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cPLA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of PLA2G4A elisa kit    PLA2G4A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705411. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PLA2G4A. It may not necessarily be applicable to this product.
 NCBI GI #    6679369
 NCBI GeneID    18783
 NCBI Accession #    NP_032895.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_008869.3 [Other Products]
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 UniProt Primary Accession #    P47713 [Other Products]
 UniProt Related Accession #    P47713 [Other Products]
 Molecular Weight    85,222 Da
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 NCBI Official Full Name    cytosolic phospholipase A2
 NCBI Official Synonym Full Names    phospholipase A2, group IVA (cytosolic, calcium-dependent)
 NCBI Official Symbol    Pla2g4a [Similar Products]
 NCBI Official Synonym Symbols   
cPLA2; Pla2g4; cPLA2alpha
[Similar Products]
 NCBI Protein Information    cytosolic phospholipase A2; cPLA2-alpha; Type IV PLA2; cytosolic PLA2; phospholipase A2, group 4; phospholipase A2 group IVA; Cytosolic phospholipase A2 (CPLA2)
 UniProt Protein Name    Cytosolic phospholipase A2
 UniProt Synonym Protein Names   
Phospholipase A2 group IVAIncluding the following 2 domains:Phospholipase A2 (EC:3.1.1.4)Alternative name(s):Phosphatidylcholine 2-acylhydrolase
 Protein Family    Cytosolic phospholipase
 UniProt Gene Name    Pla2g4a [Similar Products]
 UniProt Synonym Gene Names    Cpla2; Pla2g4; cPLA2 [Similar Products]
 UniProt Entry Name    PA24A_MOUSE
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 NCBI Summary for PLA2G4A    The protein encoded by this gene is a member of the phospholipase A2 group IV family. This enzyme hydrolyzes membrane phospholipids, thereby releasing the polyunsaturated fatty acid, arachidonic acid. Arachidonic acid is further metabolized into eicosanoids such as leukotrienes, thromboxanes and prostaglandins, that play important roles in regulating diverse biological processes such as inflammatory responses, membrane and actin dynamics, and tumorigenesis. A rise in intracellular calcium levels results in binding of calcium to the C2 domain of this protein, and triggers the translocation from the cytosol to intracellular membranes, including the Golgi apparatus. Disruption of this gene in mice led to decreased levels of eicosonaoids and platelet-activating factor, decreased allergic symptoms, and impaired reproductive ability in females. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]
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 UniProt Comments for PLA2G4A    Function: Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

Catalytic activity: Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulation: Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.
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 Research Articles on PLA2G4A    1. cPLA2 deletion ameliorates motor deficits and reduces cell loss and tissue damage after spinal cord injury.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PLA2G4A elisa kitPathways associated with PLA2G4A elisa kit
 Reference Product  PubMed Publications
 CASP3 elisa kit  >34 publications with PLA2G4A and CASP3
 PLA2G5 elisa kit  >18 publications with PLA2G4A and PLA2G5
 CASP1 elisa kit  >3 publications with PLA2G4A and CASP1
 KAT5 elisa kit  >2 publications with PLA2G4A and KAT5
 JAK1 elisa kit  >2 publications with PLA2G4A and JAK1
 VIM elisa kit  >1 publications with PLA2G4A and VIM
 MKNK1 elisa kit  >1 publications with PLA2G4A and MKNK1
 Products by Pathway  Pathway Diagram
 ADP Signalling Through P2Y Purinoceptor 1 Pathway antibodies  ADP Signalling Through P2Y Purinoceptor 1 Pathway Diagram
 Acyl Chain Remodeling Of CL Pathway antibodies  Acyl Chain Remodeling Of CL Pathway Diagram
 Acyl Chain Remodelling Of PC Pathway antibodies  Acyl Chain Remodelling Of PC Pathway Diagram
 Acyl Chain Remodelling Of PE Pathway antibodies  Acyl Chain Remodelling Of PE Pathway Diagram
 Acyl Chain Remodelling Of PG Pathway antibodies  Acyl Chain Remodelling Of PG Pathway Diagram
 Acyl Chain Remodelling Of PI Pathway antibodies  Acyl Chain Remodelling Of PI Pathway Diagram
 Acyl Chain Remodelling Of PS Pathway antibodies  Acyl Chain Remodelling Of PS Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
Diseases associated with PLA2G4A elisa kitOrgans/Tissues associated with PLA2G4A elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >303 publications with PLA2G4A and Inflammation
 Necrosis Antibodies  >171 publications with PLA2G4A and Necrosis
 Nervous System Diseases Antibodies  >142 publications with PLA2G4A and Nervous System Diseases
 Disease Models, Animal Antibodies  >116 publications with PLA2G4A and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >106 publications with PLA2G4A and Cardiovascular Diseases
 Edema Antibodies  >34 publications with PLA2G4A and Edema
 Lung Neoplasms Antibodies  >33 publications with PLA2G4A and Lung Neoplasms
 Heart Diseases Antibodies  >33 publications with PLA2G4A and Heart Diseases
 Neoplasms, Experimental Antibodies  >30 publications with PLA2G4A and Neoplasms, Experimental
 Liver Diseases Antibodies  >28 publications with PLA2G4A and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >239 publications with PLA2G4A and Brain
 Vascular Antibodies  >190 publications with PLA2G4A and Vascular
 Lung Antibodies  >171 publications with PLA2G4A and Lung
 Kidney Antibodies  >137 publications with PLA2G4A and Kidney
 Embryonic Tissue Antibodies  >59 publications with PLA2G4A and Embryonic Tissue
 Ovary Antibodies  >41 publications with PLA2G4A and Ovary
 Uterus Antibodies  >37 publications with PLA2G4A and Uterus
 Eye Antibodies  >36 publications with PLA2G4A and Eye
 Intestine Antibodies  >31 publications with PLA2G4A and Intestine
 Skin Antibodies  >29 publications with PLA2G4A and Skin
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