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POMC elisa kit :: Mouse pro-opiomelanocortin, POMC ELISA Kit

Scan QR to view Datasheet Catalog #    MBS731846
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

pro-opiomelanocortin, POMC, ELISA Kit

★Popular Item★
 Also Known As   

Mouse pro-opiomelanocortin, POMC ELISA Kit

 Product Synonym Names    Mouse pro-opiomelanocortin, POMC ELISA Kit; pro-opiomelanocortin, POMC; pro-opiomelanocortin, POMC ELISA Kit (Mouse)
 Product Gene Name   

POMC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 2; NC_000002.11 (25383722..25391559, complement). Location: 2p23.3
 3D Structure    ModBase 3D Structure for Q6FHC8
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of POMC. No significant cross-reactivity or interference between POMC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete thecross-reactivity detection between POMC and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
 Intended Uses    This POMC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse POMC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of POMC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for POMC purchase    MBS731846 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the pro-opiomelanocortin, POMC, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing POMC, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS731846 kit is based on POMC antibody-POMC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect POMC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, POMC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for POMC elisa kit

   Principle of the assay: POMC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-POMC antibody and an POMC-HRP conjugate. The assay sample and buffer are incubated together with POMC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the POMC concentration since POMC from samples and POMC-HRP conjugate compete for the anti-POMC antibody binding site. Since the number of sites is limited, as more sites are occupied by POMC from the sample, fewer sites are left to bind POMC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The POMC concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for POMC elisa kit    Mouse ELISA Kit
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 Typical Testing Data/Standard Curve (for reference only) of POMC elisa kit    POMC elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS731846. Click to request current manual
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NCBI/Uniprot data below describe general gene information for POMC. It may not necessarily be applicable to this product.
 NCBI GI #    49456609
 NCBI GeneID    5443
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 UniProt Primary Accession #    Q6FHC8 [Other Products]
 UniProt Related Accession #    P01189; Q53T23; Q53WY7; Q5TZZ7; Q6FHC8; Q6LDP0 [Other Products]
 Molecular Weight    29,410 Da
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 NCBI Official Full Name    POMC
 NCBI Official Synonym Full Names    proopiomelanocortin
 NCBI Official Symbol    POMC [Similar Products]
 NCBI Official Synonym Symbols   
LPH; MSH; NPP; POC; ACTH; CLIP
[Similar Products]
 NCBI Protein Information    pro-opiomelanocortin; beta-LPH; beta-MSH; alpha-MSH; gamma-LPH; gamma-MSH; beta-endorphin; met-enkephalin; lipotropin beta; lipotropin gamma; melanotropin beta; OTTHUMP00000119991; OTTHUMP00000200964; OTTHUMP00000200965; OTTHUMP00000200966; melanotropin alpha; melanotropin gamma; pro-ACTH-endorphin; adrenocorticotropin; corticotropin-lipotropin; adrenocorticotropic hormone; proopiomelanocortin preproprotein; beta-melanocyte-stimulating hormone; alpha-melanocyte-stimulating hormone; corticotropin-like intermediary peptide
 UniProt Protein Name    POMC protein
 Protein Family    Pro-opiomelanocortin
 UniProt Gene Name    POMC [Similar Products]
 UniProt Entry Name    Q6FHC8_HUMAN
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 NCBI Summary for POMC    This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]
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 UniProt Comments for POMC    POMC: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency and red hair. Belongs to the POMC family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: extracellular space; peroxisomal matrix; cytoplasm; extracellular region; peroxisome; secretory granule

Molecular Function: type 3 melanocortin receptor binding; G-protein-coupled receptor binding; hormone activity; type 4 melanocortin receptor binding; receptor binding

Biological Process: generation of precursor metabolites and energy; cellular protein metabolic process; cell-cell signaling; neuropeptide signaling pathway; regulation of blood pressure; peptide hormone processing; regulation of appetite; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; glucose homeostasis; signal transduction; cellular pigmentation

Disease: Obesity; Proopiomelanocortin Deficiency
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 Research Articles on POMC    1. alpha-Melanocyte-stimulating hormone inhibits tumor necrosis factor alpha-stimulated MUC5AC expression in human nasal epithelial cells
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with POMC elisa kitPathways associated with POMC elisa kit
 Reference Product  PubMed Publications
 MC4R elisa kit  >258 publications with POMC and MC4R
 MC1R elisa kit  >178 publications with POMC and MC1R
 MC2R elisa kit  >91 publications with POMC and MC2R
 MC5R elisa kit  >77 publications with POMC and MC5R
 FGF10 elisa kit  >2 publications with POMC and FGF10
 Products by Pathway  Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Biosynthesis Of Amine And Peptide Hormones Pathway antibodies  Biosynthesis Of Amine And Peptide Hormones Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 G-protein Activation Pathway antibodies  G-protein Activation Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
Diseases associated with POMC elisa kitOrgans/Tissues associated with POMC elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >7367 publications with POMC and Nervous System Diseases
 Neoplasms Antibodies  >6297 publications with POMC and Neoplasms
 PROOPIOMELANOCORTIN DEFICIENCY Antibodies  >2156 publications with POMC and PROOPIOMELANOCORTIN DEFICIENCY
 Lung Diseases Antibodies  >1712 publications with POMC and Lung Diseases
 Obesity Antibodies  >1391 publications with POMC and Obesity
 Pain Antibodies  >1266 publications with POMC and Pain
 Carcinoma Antibodies  >1264 publications with POMC and Carcinoma
 Hypertension Antibodies  >1216 publications with POMC and Hypertension
 Congenital Abnormalities Antibodies  >1033 publications with POMC and Congenital Abnormalities
 Kidney Diseases Antibodies  >961 publications with POMC and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >17030 publications with POMC and Brain
 Pituitary Gland Antibodies  >8765 publications with POMC and Pituitary Gland
 Lung Antibodies  >918 publications with POMC and Lung
 Eye Antibodies  >568 publications with POMC and Eye
 Testis Antibodies  >539 publications with POMC and Testis
 Intestine Antibodies  >402 publications with POMC and Intestine
 Placenta Antibodies  >358 publications with POMC and Placenta
 Embryonic Tissue Antibodies  >357 publications with POMC and Embryonic Tissue
 Uterus Antibodies  >236 publications with POMC and Uterus
 Uncharacterized Tissue Antibodies  >2 publications with POMC and Uncharacterized Tissue
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