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P4HB elisa kit :: Mouse prolyl 4-hydroxylase, beta polypeptide ELISA Kit

Scan QR to view Datasheet Catalog #    MBS933098
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

prolyl 4-hydroxylase, beta polypeptide (P4HB), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Protein disulfide-isomerase, P4HB ELISA Kit

 Product Synonym Names    Mouse Protein disulfide-isomerase (P4HB) ELISA kit; DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB; collagen prolyl 4-hydroxylase beta; glutathione-insulin transhydrogenase; procollagen-proline; 2-oxoglutarate 4-dioxygenase (proline 4; prolyl 4-hydroxylase; beta polypeptide
 Product Gene Name   

P4HB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS933098 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P09103
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse P4HB. No significant cross-reactivity or interference between Mouse P4HB and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Detection Range    0.312 ng/ml-20 ng/ml
 Sensitivity    0.078 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of P4HB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for P4HB purchase    MBS933098 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the prolyl 4-hydroxylase, beta polypeptide (P4HB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml-20 ng/ml in biological research samples containing P4HB, with an estimated sensitivity of 0.078 ng/ml. The ELISA analytical biochemical technique of the MBS933098 kit is based on P4HB antibody-P4HB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect P4HB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, P4HB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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 Typical Testing Data/Standard Curve (for reference only) of P4HB elisa kit    P4HB elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS933098. Click to request current manual
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NCBI/Uniprot data below describe general gene information for P4HB. It may not necessarily be applicable to this product.
 NCBI GI #    42415475
 NCBI GeneID    18453
 NCBI Accession #    NP_035162.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_011032.2 [Other Products]
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 UniProt Primary Accession #    P09103 [Other Products]
 UniProt Secondary Accession #    Q922C8 [Other Products]
 UniProt Related Accession #    P09103 [Other Products]
 Molecular Weight    57,058 Da
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 NCBI Official Full Name    protein disulfide-isomerase
 NCBI Official Synonym Full Names    prolyl 4-hydroxylase, beta polypeptide
 NCBI Official Symbol    P4hb [Similar Products]
 NCBI Official Synonym Symbols   
PDI; Thbp; ERp59; Pdia1
[Similar Products]
 NCBI Protein Information    protein disulfide-isomerase; p55; ER protein 59; protein disulfide isomerase; prolyl 4-hydroxylase subunit beta; cellular thyroid hormone-binding protein; endoplasmic reticulum resident protein 59
 UniProt Protein Name    Protein disulfide-isomerase
 UniProt Synonym Protein Names   
Cellular thyroid hormone-binding protein; Endoplasmic reticulum resident protein 59; ER protein 59; ERp59; Prolyl 4-hydroxylase subunit beta; p55
 UniProt Gene Name    P4hb [Similar Products]
 UniProt Synonym Gene Names    Pdia1; PDI; ER protein 59; ERp59 [Similar Products]
 UniProt Entry Name    PDIA1_MOUSE
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 UniProt Comments for P4HB    PDIA1: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Homodimer. Monomers and homotetramers may also occur. Also constitutes the structural subunit of prolyl 4-hydroxylase and of the microsomal triacylglycerol transfer protein MTTP in mammalian cells. Stabilizes both enzymes and retain them in the ER without contributing to the catalytic activity. Binds UBQLN1. Binds to CD4, and upon HIV-1 binding to the cell membrane, is part of a P4HB/PDI-CD4-CXCR4-gp120 complex. Belongs to the protein disulfide isomerase family.

Protein type: Nuclear receptor co-regulator; Oxidoreductase; Endoplasmic reticulum; EC 5.3.4.1; Isomerase

Cellular Component: focal adhesion; membrane; endoplasmic reticulum; plasma membrane; ER-Golgi intermediate compartment

Molecular Function: enzyme binding; procollagen-proline 4-dioxygenase activity; protein heterodimerization activity; protein disulfide isomerase activity; isomerase activity; peptidyl-proline 4-dioxygenase activity

Biological Process: protein folding; cell redox homeostasis; peptidyl-proline hydroxylation to 4-hydroxy-L-proline
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 Research Articles on P4HB    1. Neutrophil surface PDI is important for alphaMbeta2 integrin-mediated adhesion of human neutrophils under shear and static conditions and for binding of soluble fibrinogen to activated alphaMbeta2 integrin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with P4HB elisa kitPathways associated with P4HB elisa kit
 Reference Product  PubMed Publications
 Ero1lb elisa kit  >1 publications with P4HB and Ero1lb
 Col1a1 elisa kit  >1 publications with P4HB and Col1a1
 Leprel1 elisa kit  >1 publications with P4HB and Leprel1
 Ero1l elisa kit  >1 publications with P4HB and Ero1l
 Products by Pathway  Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Collagen Biosynthesis And Modifying Enzymes Pathway antibodies  Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 Detoxification Of Reactive Oxygen Species Pathway antibodies  Detoxification Of Reactive Oxygen Species Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hedgehog Ligand Biogenesis Pathway antibodies  Hedgehog Ligand Biogenesis Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Lipoprotein Metabolism Pathway antibodies  Lipoprotein Metabolism Pathway Diagram
Diseases associated with P4HB elisa kitOrgans/Tissues associated with P4HB elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >6 publications with P4HB and Nervous System Diseases
 Inflammation Antibodies  >5 publications with P4HB and Inflammation
 Cardiovascular Diseases Antibodies  >3 publications with P4HB and Cardiovascular Diseases
 Necrosis Antibodies  >3 publications with P4HB and Necrosis
 Fibrosis Antibodies  >3 publications with P4HB and Fibrosis
 Immune System Diseases Antibodies  >2 publications with P4HB and Immune System Diseases
 Myocardial Ischemia Antibodies  >2 publications with P4HB and Myocardial Ischemia
 Weight Loss Antibodies  >1 publications with P4HB and Weight Loss
 Heart Diseases Antibodies  >1 publications with P4HB and Heart Diseases
 Breast Neoplasms Antibodies  >1 publications with P4HB and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >11 publications with P4HB and Heart
 Blood Antibodies  >10 publications with P4HB and Blood
 Muscle Antibodies  >8 publications with P4HB and Muscle
 Embryonic Tissue Antibodies  >6 publications with P4HB and Embryonic Tissue
 Bone Antibodies  >5 publications with P4HB and Bone
 Bone Marrow Antibodies  >4 publications with P4HB and Bone Marrow
 Connective Tissue Antibodies  >3 publications with P4HB and Connective Tissue
 Thyroid Antibodies  >2 publications with P4HB and Thyroid
 Testis Antibodies  >1 publications with P4HB and Testis
 Joint Antibodies  >1 publications with P4HB and Joint
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