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ROBO4 elisa kit :: Mouse roundabout homolog 4, magic roundabout (Drosophila) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS939371
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
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 Product Name   

roundabout homolog 4, magic roundabout (Drosophila) (ROBO4), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Roundabout homolog 4, ROBO4 ELISA Kit

 Product Synonym Names    Mouse Roundabout homolog 4 (ROBO4) ELISA kit; ECSM4; FLJ20798; MGC133352; MGC133353; MRB; roundabout homolog 4; magic roundabout; roundabout homolog 4; magic roundabout (Drosophila)
 Product Gene Name   

ROBO4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS939371 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q8C310
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse ROBO4. No significant cross-reactivity or interference between Mouse ROBO4 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.156 ng/ml-10 ng/ml
 Sensitivity    0.039 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Angiogenesis
 Protein Biological Process 3    Angiogenesis
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ROBO4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ROBO4 purchase    MBS939371 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the roundabout homolog 4, magic roundabout (Drosophila) (ROBO4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 ng/ml-10 ng/ml in biological research samples containing ROBO4, with an estimated sensitivity of 0.039 ng/ml. The ELISA analytical biochemical technique of the MBS939371 kit is based on ROBO4 antibody-ROBO4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ROBO4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ROBO4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ROBO4 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ROBO4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ROBO4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ROBO4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ROBO4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ROBO4 elisa kit    ROBO4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS939371. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ROBO4. It may not necessarily be applicable to this product.
 NCBI GI #    27229187
 NCBI GeneID    74144
 NCBI Accession #    NP_083059.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_028783.2 [Other Products]
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 UniProt Primary Accession #    Q8C310 [Other Products]
 UniProt Secondary Accession #    Q9DBW1 [Other Products]
 UniProt Related Accession #    Q8C310 [Other Products]
 Molecular Weight    108,499 Da
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 NCBI Official Full Name    roundabout homolog 4
 NCBI Official Synonym Full Names    roundabout homolog 4 (Drosophila)
 NCBI Official Symbol    Robo4 [Similar Products]
 NCBI Official Synonym Symbols   
AI593217; 1200012D01Rik
[Similar Products]
 NCBI Protein Information    roundabout homolog 4; Magic roundabout
 UniProt Protein Name    Roundabout homolog 4
 Protein Family    Roundabout
 UniProt Gene Name    Robo4 [Similar Products]
 UniProt Entry Name    ROBO4_MOUSE
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 UniProt Comments for ROBO4    ROBO4: Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Belongs to the immunoglobulin superfamily. ROBO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: multicellular organismal development; angiogenesis; cell differentiation; negative regulation of cell migration
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 Research Articles on ROBO4    1. These results clearly indicate that Robo4 plays a role in hematopoietic stem cells trafficking such as bone marrow homing and peripheral mobilization.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with ROBO4 elisa kitOrgans/Tissues associated with ROBO4 elisa kit
 Disease Name  Pubmed Publications
 Endocrine System Diseases Antibodies  >3 publications with ROBO4 and Endocrine System Diseases
 Syndrome Antibodies  >3 publications with ROBO4 and Syndrome
 Carcinoma in Situ Antibodies  >2 publications with ROBO4 and Carcinoma in Situ
 Ovarian Neoplasms Antibodies  >2 publications with ROBO4 and Ovarian Neoplasms
 Ovarian Diseases Antibodies  >2 publications with ROBO4 and Ovarian Diseases
 Gonadal Disorders Antibodies  >2 publications with ROBO4 and Gonadal Disorders
 Translocation, Genetic Antibodies  >1 publications with ROBO4 and Translocation, Genetic
 Breast Neoplasms Antibodies  >1 publications with ROBO4 and Breast Neoplasms
 Edema Antibodies  >1 publications with ROBO4 and Edema
 Liver Diseases Antibodies  >1 publications with ROBO4 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >11 publications with ROBO4 and Embryonic Tissue
 Eye Antibodies  >10 publications with ROBO4 and Eye
 Brain Antibodies  >4 publications with ROBO4 and Brain
 Muscle Antibodies  >3 publications with ROBO4 and Muscle
 Bone Antibodies  >3 publications with ROBO4 and Bone
 Bone Marrow Antibodies  >3 publications with ROBO4 and Bone Marrow
 Lung Antibodies  >2 publications with ROBO4 and Lung
 Kidney Antibodies  >1 publications with ROBO4 and Kidney
 Liver Antibodies  >1 publications with ROBO4 and Liver
 Mammary Gland Antibodies  >1 publications with ROBO4 and Mammary Gland
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