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RUNX2 elisa kit :: Mouse runt-related transcription factor 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS934869
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

runt-related transcription factor 2 (RUNX2), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Runt-related transcription factor 2, RUNX2 ELISA Kit

 Product Synonym Names    Mouse Runt-related transcription factor 2 (RUNX2) ELISA kit; RP1-166H4.1; AML3; CBFA1; CCD; CCD1; MGC120022; MGC120023; OSF2; PEA2aA; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1; CBF-alpha 1; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A; runt-related transcription factor 2
 Product Gene Name   

RUNX2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS934869 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q08775
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse RUNX2. No significant cross-reactivity or interference between mouse RUNX2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    3.12 ng/ml -200 ng/ml.
 Sensitivity    The minimum detectable dose of mouse RUNX2 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RUNX2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RUNX2 purchase    MBS934869 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the runt-related transcription factor 2 (RUNX2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12 ng/ml -200 ng/ml in biological research samples containing RUNX2, with an estimated sensitivity of The minimum detectable dose of mouse RUNX2 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS934869 kit is based on RUNX2 antibody-RUNX2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RUNX2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RUNX2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RUNX2 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RUNX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RUNX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RUNX2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RUNX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of RUNX2 elisa kit    RUNX2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS934869. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RUNX2. It may not necessarily be applicable to this product.
 NCBI GI #    225690526
 NCBI GeneID    12393
 NCBI Accession #    NP_001139510.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001146038.2 [Other Products]
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 UniProt Primary Accession #    Q08775 [Other Products]
 UniProt Secondary Accession #    O35183; Q08776; Q9JLN0; Q9QUQ6; Q9QY29; Q9R0U4; Q9Z2J7 [Other Products]
 UniProt Related Accession #    Q08775 [Other Products]
 Molecular Weight    66,205 Da
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 NCBI Official Full Name    runt-related transcription factor 2 isoform 1
 NCBI Official Synonym Full Names    runt related transcription factor 2
 NCBI Official Symbol    Runx2 [Similar Products]
 NCBI Official Synonym Symbols   
Cbf; LS3; AML3; Osf2; Cbfa1; Cbfa-1; PEBP2aA; Pebp2a1; Pebpa2a
[Similar Products]
 NCBI Protein Information    runt-related transcription factor 2; CBF-alpha-1; PEA2-alpha A; PEBP2 alpha A; AKV core binding factor; core binding factor alpha 1; runt domain, alpha subunit 1; acute myeloid leukemia 3 protein; SL3-3 enhancer factor 1 alpha A subunit; osteoblast-specific transcription factor 2; polyomavirus enhancer-binding protein 2 alpha A subunit
 UniProt Protein Name    Runt-related transcription factor 2
 UniProt Synonym Protein Names   
Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit
 Protein Family    Runt-related transcription factor
 UniProt Gene Name    Runx2 [Similar Products]
 UniProt Synonym Gene Names    Aml3; Cbfa1; Osf2; Pebp2a; CBF-alpha-1; OSF-2; PEA2-alpha A; PEBP2-alpha A [Similar Products]
 UniProt Entry Name    RUNX2_MOUSE
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 UniProt Comments for RUNX2    AML3: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors. Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 and HIPK3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with KAT6A and KAT6B. Binds to cyclin B1 CCNB1. Interacts with DDX5. Specifically expressed in osteoblasts. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; nucleus

Molecular Function: protein domain specific binding; protein binding; DNA binding; histone deacetylase binding; bHLH transcription factor binding; chromatin binding; transcription factor binding; transcription factor activity; ATP binding

Biological Process: transcription from RNA polymerase II promoter; embryonic forelimb morphogenesis; positive regulation of transcription, DNA-dependent; cell maturation; neuron differentiation; BMP signaling pathway; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; chondrocyte differentiation; skeletal development; regulation of odontogenesis of dentine-containing teeth; T cell differentiation; endochondral ossification; regulation of osteoblast differentiation; transcription, DNA-dependent; regulation of fibroblast growth factor receptor signaling pathway; embryonic cranial skeleton morphogenesis; stem cell differentiation; chondrocyte development; positive regulation of ossification; odontogenesis of dentine-containing teeth; osteoblast development; osteoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of chondrocyte differentiation; skeletal morphogenesis; negative regulation of smoothened signaling pathway; positive regulation of transcription from RNA polymerase II promoter; regulation of ossification; negative regulation of transcription, DNA-dependent; osteoblast fate commitment
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 Research Articles on RUNX2    1. miR-338-3p plays an important role during osteoblastic differentiation of bone marrow stromal stem cells by targeting Runx2 and Fgfr2.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RUNX2 elisa kitPathways associated with RUNX2 elisa kit
 Reference Product  PubMed Publications
 Hdac4 elisa kit  >18 publications with RUNX2 and Hdac4
 Rb1 elisa kit  >5 publications with RUNX2 and Rb1
 Products by Pathway  Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 TGF Beta Signaling Pathway antibodies  TGF Beta Signaling Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Wnt Signaling Pathway NetPath antibodies  Wnt Signaling Pathway NetPath Diagram
Diseases associated with RUNX2 elisa kitOrgans/Tissues associated with RUNX2 elisa kit
 Disease Name  Pubmed Publications
 Bone Diseases Antibodies  >421 publications with RUNX2 and Bone Diseases
 Neoplasms Antibodies  >205 publications with RUNX2 and Neoplasms
 Osteoporosis Antibodies  >168 publications with RUNX2 and Osteoporosis
 Craniofacial Abnormalities Antibodies  >114 publications with RUNX2 and Craniofacial Abnormalities
 Cleidocranial Dysplasia Antibodies  >113 publications with RUNX2 and Cleidocranial Dysplasia
 Necrosis Antibodies  >74 publications with RUNX2 and Necrosis
 Leukemia Antibodies  >65 publications with RUNX2 and Leukemia
 Cardiovascular Diseases Antibodies  >59 publications with RUNX2 and Cardiovascular Diseases
 Inflammation Antibodies  >56 publications with RUNX2 and Inflammation
 Breast Neoplasms Antibodies  >31 publications with RUNX2 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Epididymis Antibodies  >1 publications with RUNX2 and Epididymis
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