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SFRP4 elisa kit :: Mouse secreted frizzled-related protein 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS907810
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

secreted frizzled-related protein 4 (SFRP4), ELISA Kit

 Also Known As   

Mouse Secreted frizzled-related protein 4, SFRP4 ELISA Kit

 Product Synonym Names    Mouse Secreted frizzled-related protein 4 (SFRP4) ELISA kit; FRP-4; FRPHE; MGC26498; secreted frizzled-related protein 4; secreted frizzled-related protein 4; secreted frizzled-related protein 4; secreted frizzled-related protein 4
 Product Gene Name   

SFRP4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS907810 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q9Z1N6
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse SFRP4. No significant cross-reactivity or interference between Mouse SFRP4 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.47 ng/ml-30 ng/ml
 Sensitivity    0.11 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Developmental Protein
 Protein Biological Process 3    Differentiation
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SFRP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SFRP4 purchase    MBS907810 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the secreted frizzled-related protein 4 (SFRP4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.47 ng/ml-30 ng/ml in biological research samples containing SFRP4, with an estimated sensitivity of 0.11 ng/ml. The ELISA analytical biochemical technique of the MBS907810 kit is based on SFRP4 antibody-SFRP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SFRP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SFRP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for SFRP4 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFRP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFRP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of SFRP4 elisa kit    SFRP4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS907810. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SFRP4. It may not necessarily be applicable to this product.
 NCBI GI #    7710094
 NCBI GeneID    20379
 NCBI Accession #    NP_057896.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_016687.3 [Other Products]
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 UniProt Primary Accession #    Q9Z1N6 [Other Products]
 UniProt Secondary Accession #    Q91ZX9 [Other Products]
 UniProt Related Accession #    Q9Z1N6 [Other Products]
 Molecular Weight    40,342 Da
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 NCBI Official Full Name    secreted frizzled-related sequence protein 4
 NCBI Official Synonym Full Names    secreted frizzled-related protein 4
 NCBI Official Symbol    Sfrp4 [Similar Products]
 NCBI Protein Information    secreted frizzled-related sequence protein 4; FRP-4; sFRP-4; frizzled-related protein 4
 UniProt Protein Name    Secreted frizzled-related sequence protein 4
 Protein Family    Secreted frizzled-related protein
 UniProt Gene Name    Sfrp4 [Similar Products]
 UniProt Synonym Gene Names    FRP-4; sFRP-4 [Similar Products]
 UniProt Entry Name    SFRP4_MOUSE
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 UniProt Comments for SFRP4    SFRP4: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cell surface; cytoplasm; extracellular region; nucleus

Molecular Function: Wnt-protein binding

Biological Process: negative regulation of JNK cascade; negative regulation of cell proliferation; Wnt receptor signaling pathway; positive regulation of apoptosis; negative regulation of transcription factor activity; multicellular organismal development; positive regulation of epidermal cell differentiation; positive regulation of receptor internalization; phosphate ion homeostasis; cell differentiation
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 Research Articles on SFRP4    1. studies indicate that mutations neither in sFRP1 nor in sFRP4 are a common cause of craniotubular hyperostoses
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with SFRP4 elisa kitDiseases associated with SFRP4 elisa kit
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >84 publications with SFRP4 and Neoplasms
 Carcinoma Antibodies  >37 publications with SFRP4 and Carcinoma
 Adenocarcinoma Antibodies  >17 publications with SFRP4 and Adenocarcinoma
 Leukemia Antibodies  >15 publications with SFRP4 and Leukemia
 Uterine Neoplasms Antibodies  >12 publications with SFRP4 and Uterine Neoplasms
 Carcinoma, Squamous Cell Antibodies  >10 publications with SFRP4 and Carcinoma, Squamous Cell
 Breast Neoplasms Antibodies  >9 publications with SFRP4 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >9 publications with SFRP4 and Cell Transformation, Neoplastic
 Colorectal Neoplasms Antibodies  >9 publications with SFRP4 and Colorectal Neoplasms
 Endometrial Neoplasms Antibodies  >8 publications with SFRP4 and Endometrial Neoplasms
Organs/Tissues associated with SFRP4 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >28 publications with SFRP4 and Bone
 Eye Antibodies  >18 publications with SFRP4 and Eye
 Uterus Antibodies  >10 publications with SFRP4 and Uterus
 Connective Tissue Antibodies  >9 publications with SFRP4 and Connective Tissue
 Joint Antibodies  >6 publications with SFRP4 and Joint
 Embryonic Tissue Antibodies  >6 publications with SFRP4 and Embryonic Tissue
 Bladder Antibodies  >5 publications with SFRP4 and Bladder
 Brain Antibodies  >5 publications with SFRP4 and Brain
 Skin Antibodies  >4 publications with SFRP4 and Skin
 Pituitary Gland Antibodies  >2 publications with SFRP4 and Pituitary Gland
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