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SPON1 elisa kit :: Mouse spondin 1, extracellular matrix protein ELISA Kit

Scan QR to view Datasheet Catalog #    MBS934854
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

spondin 1, extracellular matrix protein (SPON1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Spondin-1, SPON1 ELISA Kit

 Product Synonym Names    Mouse Spondin-1 (SPON1) ELISA kit; KIAA0762; MGC10724; f-spondin; VSGP/F-spondin; spondin 1; (f-spondin) extracellular matrix protein; spondin 1; extracellular matrix protein
 Product Gene Name   

SPON1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS934854 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q8VCC9
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse SPON1. No significant cross-reactivity or interference between mouse SPON1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Quantitative Sandwich
 Detection Range    0.156 ng/ml -10 ng/ml
 Sensitivity    Typically less than 0.039 ng/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SPON1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SPON1 purchase    MBS934854 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the spondin 1, extracellular matrix protein (SPON1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 ng/ml -10 ng/ml in biological research samples containing SPON1, with an estimated sensitivity of Typically less than 0.039 ng/ml. The ELISA analytical biochemical technique of the MBS934854 kit is based on SPON1 antibody-SPON1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPON1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPON1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SPON1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPON1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPON1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPON1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPON1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of SPON1 elisa kit    SPON1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS934854. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SPON1. It may not necessarily be applicable to this product.
 NCBI GI #    21704174
 NCBI GeneID    233744
 NCBI Accession #    NP_663559.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_145584.2 [Other Products]
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 UniProt Primary Accession #    Q8VCC9 [Other Products]
 UniProt Secondary Accession #    Q8K2Q8 [Other Products]
 UniProt Related Accession #    Q8VCC9 [Other Products]
 Molecular Weight    90,821 Da
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 NCBI Official Full Name    spondin-1
 NCBI Official Synonym Full Names    spondin 1, (f-spondin) extracellular matrix protein
 NCBI Official Symbol    Spon1 [Similar Products]
 NCBI Official Synonym Symbols   
FSP; AI666765; AW455831; BC020531; D330035F22Rik
[Similar Products]
 NCBI Protein Information    spondin-1; F-spondin
 UniProt Protein Name    Spondin-1
 UniProt Synonym Protein Names   
F-spondin
 Protein Family    Spondin
 UniProt Gene Name    Spon1 [Similar Products]
 UniProt Entry Name    SPON1_MOUSE
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 UniProt Comments for SPON1    SPON1: Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Biological Process: cell adhesion
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 Research Articles on SPON1    1. F-spondin is expressed in embryonic cartilage, where it has the capacity to enhance chondrocyte terminal differentiation and mineralization.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with SPON1 elisa kitOrgans/Tissues associated with SPON1 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >5 publications with SPON1 and Neoplasms
 Carcinoma Antibodies  >4 publications with SPON1 and Carcinoma
 Ovarian Neoplasms Antibodies  >4 publications with SPON1 and Ovarian Neoplasms
 Hypertension Antibodies  >3 publications with SPON1 and Hypertension
 Adenocarcinoma Antibodies  >2 publications with SPON1 and Adenocarcinoma
 Nervous System Diseases Antibodies  >2 publications with SPON1 and Nervous System Diseases
 Skin Diseases Antibodies  >2 publications with SPON1 and Skin Diseases
 Necrosis Antibodies  >2 publications with SPON1 and Necrosis
 Brain Diseases Antibodies  >1 publications with SPON1 and Brain Diseases
 Neoplasm Metastasis Antibodies  >1 publications with SPON1 and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >5 publications with SPON1 and Embryonic Tissue
 Brain Antibodies  >4 publications with SPON1 and Brain
 Kidney Antibodies  >2 publications with SPON1 and Kidney
 Eye Antibodies  >2 publications with SPON1 and Eye
 Connective Tissue Antibodies  >1 publications with SPON1 and Connective Tissue
 Joint Antibodies  >1 publications with SPON1 and Joint
 Pancreas Antibodies  >1 publications with SPON1 and Pancreas
 Heart Antibodies  >1 publications with SPON1 and Heart
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