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SOD1 elisa kit :: Mouse superoxide dismutase 1, soluble ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705047
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $575 +1 FREE 8GB USB
96-Strip-Wells  /  $835 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

superoxide dismutase 1, soluble (SOD1), ELISA Kit

 Also Known As   

Mouse Super Oxidase Dimutase, SOD ELISA Kit

 Product Synonym Names    Mouse Super Oxidase Dimutase; SOD ELISA Kit; ALS; ALS1; IPOA; SOD; homodimer; Cu/Zn superoxide dismutase; SOD; soluble; indophenoloxidase A; superoxide dismutase; cystolic; superoxide dismutase 1; soluble
 Product Gene Name   

SOD1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705047 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P08228
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse SOD1. No significant cross-reactivity or interference between Mouse SOD1 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates and tissue homogenates
 Detection Range    0.156 ng/ml-10 ng/ml
 Sensitivity    0.039 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SOD1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SOD1 purchase    MBS705047 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the superoxide dismutase 1, soluble (SOD1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 ng/ml-10 ng/ml in biological research samples containing SOD1, with an estimated sensitivity of 0.039 ng/ml. The ELISA analytical biochemical technique of the MBS705047 kit is based on SOD1 antibody-SOD1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SOD1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SOD1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates and tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for SOD1 elisa kit

   Introduction: The enzyme superoxide dismutase (SOD), catalyzes the dismutation of superoxide into oxygen and hydrogen peroxide. As such, it is an important antioxidant defense in nearly all cells exposed to oxygen. Several common forms of SOD exist: they are proteins cofactored with copper and zinc, or manganese, iron, or nickel. The cytosols of virtually all eukaryotic cells contain an SOD enzyme with copper and zinc (Cu-Zn-SOD). The Cu-Zn enzyme is a homodimer of molecular weight 32,500. The two subunits are joined primarily by hydrophobic and electrostatic interactions. The ligands of copper and zinc are histidine side chains. Chicken liver (and nearly all other) mitochondria, and many bacteria contain a form with manganese (Mn-SOD). The ligands of the manganese ions are 3 histidine side chains, an aspartate side chain and a water molecule or hydroxy ligand depending on the Mn oxidation state (respectively II and III). E. coli and many other bacteria also contain a form of the enzyme with iron (Fe-SOD); some bacteria contain Fe-SOD, others Mn-SOD, and some contain both. Fe-SOD can be found in the plastids of plants. The active sites of Mn and Fe superoxide dismutases contain the same type of amino acid side chains. Simply stated, SOD outcompetes damaging reactions of superoxide, thus protecting the cell from superoxide toxicity. The reaction of superoxide with non-radicals is spin forbidden. In biological systems, this means its main reactions are with itself or with another biological radical such as nitric oxide (NO). The superoxide anion radical (O2-) spontaneously dismutes to O2 and hydrogen peroxide (H2O2) quite rapidly. SOD is biologically necessary because superoxide reacts even faster with certain targets such as NO radical, which makes peroxynitrite. Similarly, the dismutation rate is second order with respect to initial superoxide concentration. Thus, the half-life of superoxide, although very short at high concentrations is actually quite long at low concentrations. In contrast, the reaction of superoxide with SOD is first order with respect to superoxide concentration. Moreover, superoxide dismutase has the fastest turnover number of any known enzyme, this reaction being only limited by the frequency of collision between itself and superoxide.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SOD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SOD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain SOD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SOD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of SOD1 elisa kit    SOD1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705047. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SOD1. It may not necessarily be applicable to this product.
 NCBI GI #    45597447
 NCBI GeneID    20655
 NCBI Accession #    NP_035564.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_011434.1 [Other Products]
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 UniProt Primary Accession #    P08228 [Other Products]
 UniProt Related Accession #    P08228 [Other Products]
 Molecular Weight    15,943 Da
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 NCBI Official Full Name    superoxide dismutase
 NCBI Official Synonym Full Names    superoxide dismutase 1, soluble
 NCBI Official Symbol    Sod1 [Similar Products]
 NCBI Official Synonym Symbols   
Ipo1; SODC; Ipo-1; Sod-1; CuZnSOD; Cu/Zn-SOD; B430204E11Rik
[Similar Products]
 NCBI Protein Information    superoxide dismutase [Cu-Zn]; superoxide dismutase [Cu-Zn]; Cu(2+)-Zn2+ superoxide dismutase
 UniProt Protein Name    Superoxide dismutase [Cu-Zn]
 Protein Family    Superoxide dismutase
 UniProt Gene Name    Sod1 [Similar Products]
 UniProt Entry Name    SODC_MOUSE
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 UniProt Comments for SOD1    Function: Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

Catalytic activity: 2 superoxide + 2 H+ = O2 + H2O2.

Cofactor: Binds 1 copper ion per subunit
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 Research Articles on SOD1    1. We used a superoxide dismutase knockout mouse to demonstrate that oxidative stress directly impairs investment in morphological (preputial glands) and molecular (major urinary proteins) components of olfactory signaling essential for mate attraction.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SOD1 elisa kitPathways associated with SOD1 elisa kit
 Reference Product  PubMed Publications
 Sod2 elisa kit  >384 publications with SOD1 and Sod2
 Cat elisa kit  >178 publications with SOD1 and Cat
 Rnf19a elisa kit  >4 publications with SOD1 and Rnf19a
 Hspa1a elisa kit  >3 publications with SOD1 and Hspa1a
 Esr1 elisa kit  >1 publications with SOD1 and Esr1
 Products by Pathway  Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Detoxification Of Reactive Oxygen Species Pathway antibodies  Detoxification Of Reactive Oxygen Species Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 One Carbon Metabolism And Related Pathways antibodies  One Carbon Metabolism And Related Pathways Diagram
 Oxidative Stress Pathway antibodies  Oxidative Stress Pathway Diagram
 Peroxisome Pathway antibodies  Peroxisome Pathway Diagram
Diseases associated with SOD1 elisa kitOrgans/Tissues associated with SOD1 elisa kit
 Disease Name  Pubmed Publications
 Amyotrophic Lateral Sclerosis Antibodies  >2399 publications with SOD1 and Amyotrophic Lateral Sclerosis
 Nervous System Diseases Antibodies  >2338 publications with SOD1 and Nervous System Diseases
 Motor Neuron Disease Antibodies  >2141 publications with SOD1 and Motor Neuron Disease
 Disease Models, Animal Antibodies  >996 publications with SOD1 and Disease Models, Animal
 Nerve Degeneration Antibodies  >307 publications with SOD1 and Nerve Degeneration
 Protein Deficiency Antibodies  >168 publications with SOD1 and Protein Deficiency
 Inflammation Antibodies  >158 publications with SOD1 and Inflammation
 Ischemia Antibodies  >136 publications with SOD1 and Ischemia
 Necrosis Antibodies  >102 publications with SOD1 and Necrosis
 Brain Ischemia Antibodies  >80 publications with SOD1 and Brain Ischemia
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with SOD1 and Bone
 Connective Tissue Antibodies  >4 publications with SOD1 and Connective Tissue
 Heart Antibodies  >3 publications with SOD1 and Heart
 Muscle Antibodies  >3 publications with SOD1 and Muscle
 Brain Antibodies  >3 publications with SOD1 and Brain
 Vascular Antibodies  >2 publications with SOD1 and Vascular
 Embryonic Tissue Antibodies  >2 publications with SOD1 and Embryonic Tissue
 Ear Antibodies  >1 publications with SOD1 and Ear
 Kidney Antibodies  >1 publications with SOD1 and Kidney
 Umbilical Cord Antibodies  >1 publications with SOD1 and Umbilical Cord
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