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SFTPA1 elisa kit :: Mouse surfactant protein A1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702358
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

surfactant protein A1 (SFTPA1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Pulmonary surfactant-associated protein A, SP-A ELISA Kit

 Product Synonym Names    Mouse Pulmonary surfactant-associated protein A; SP-A ELISA Kit; AC068139.6; MGC133365; PSAP; PSPA; SFTP1; SFTPA1; pulmonary surfactant apoprotein; surfactant; pulmonary-associated protein A1; surfactant; pulmonary-associated protein A1B; surfactant protein A1
 Product Gene Name   

SFTPA1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS702358 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P35242
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse SP-A. No significant cross-reactivity or interference between mouse SP-A and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    3.12 pg/ml -200 pg/ml.
 Sensitivity    The minimum detectable dose of mouse SP-A is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SFTPA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SFTPA1 purchase    MBS702358 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the surfactant protein A1 (SFTPA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12 pg/ml -200 pg/ml in biological research samples containing SFTPA1, with an estimated sensitivity of The minimum detectable dose of mouse SP-A is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS702358 kit is based on SFTPA1 antibody-SFTPA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SFTPA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SFTPA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SFTPA1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-A bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of SFTPA1 elisa kit    SFTPA1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS702358. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SFTPA1. It may not necessarily be applicable to this product.
 NCBI GI #    464483
 NCBI GeneID    20387
 NCBI Accession #    P35242.1 [Other Products]
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 UniProt Primary Accession #    P35242 [Other Products]
 UniProt Related Accession #    P35242 [Other Products]
 Molecular Weight    26,157 Da
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 NCBI Official Full Name    Pulmonary surfactant-associated protein A
 NCBI Official Synonym Full Names    surfactant associated protein A1
 NCBI Official Symbol    Sftpa1 [Similar Products]
 NCBI Official Synonym Symbols   
SP-A; Sftp1; Sftp-1
[Similar Products]
 NCBI Protein Information    pulmonary surfactant-associated protein A; PSAP; PSP-A; surfactant pulmonary associated protein A1
 UniProt Protein Name    Pulmonary surfactant-associated protein A
 Protein Family    Pulmonary surfactant-associated protein
 UniProt Gene Name    Sftpa1 [Similar Products]
 UniProt Synonym Gene Names    Sftp-1; Sftp1; Sftpa; PSAP; PSP-A; SP-A [Similar Products]
 UniProt Entry Name    SFTPA_MOUSE
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 UniProt Comments for SFTPA1    Function: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.
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 Research Articles on SFTPA1    1. peptide from the interface with TLR4 inhibits endotoxic shock-like symptoms in a mouse model
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SFTPA1 elisa kitPathways associated with SFTPA1 elisa kit
 Reference Product  PubMed Publications
 Sftpd elisa kit  >8 publications with SFTPA1 and Sftpd
 Sftpb elisa kit  >7 publications with SFTPA1 and Sftpb
 Sftpc elisa kit  >6 publications with SFTPA1 and Sftpc
 Scgb1a1 elisa kit  >1 publications with SFTPA1 and Scgb1a1
 Scgb3a2 elisa kit  >1 publications with SFTPA1 and Scgb3a2
 Products by Pathway  Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
Organs/Tissues associated with SFTPA1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >62 publications with SFTPA1 and Lung
 Embryonic Tissue Antibodies  >6 publications with SFTPA1 and Embryonic Tissue
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