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THBS1 elisa kit :: Mouse thrombospondin 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704061
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $575 +1 FREE 8GB USB
96-Strip-Wells  /  $835 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

thrombospondin 1 (THBS1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse thrombospondin 1, TSP-1 ELISA Kit

 Product Synonym Names    Mouse thrombospondin 1; TSP-1 ELISA Kit; THBS; THBS-1; TSP; TSP-1; TSP1; thrombospondin-1p180; thrombospondin 1
 Product Gene Name   

THBS1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704061 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P35441
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse TSP-1. No significant cross-reactivity or interference between mouse TSP-1 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates.
 Assay Type    Sandwich (Quantitative)
 Detection Range    7.8 pg/ml -500 pg/ml.
 Sensitivity    1.95 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of THBS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for THBS1 purchase    MBS704061 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the thrombospondin 1 (THBS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.8 pg/ml -500 pg/ml in biological research samples containing THBS1, with an estimated sensitivity of 1.95 pg/ml. The ELISA analytical biochemical technique of the MBS704061 kit is based on THBS1 antibody-THBS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect THBS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, THBS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for THBS1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TSP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of THBS1 elisa kit    THBS1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704061. Click to request current manual
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NCBI/Uniprot data below describe general gene information for THBS1. It may not necessarily be applicable to this product.
 NCBI GI #    549134
 NCBI GeneID    21825
 NCBI Accession #    P35441.1 [Other Products]
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 UniProt Primary Accession #    P35441 [Other Products]
 UniProt Related Accession #    P35441 [Other Products]
 Molecular Weight    129,647 Da
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 NCBI Official Full Name    Thrombospondin-1
 NCBI Official Synonym Full Names    thrombospondin 1
 NCBI Official Symbol    Thbs1 [Similar Products]
 NCBI Official Synonym Symbols   
TSP1; TSP-1; tbsp1; Thbs-1
[Similar Products]
 NCBI Protein Information    thrombospondin-1
 UniProt Protein Name    Thrombospondin-1
 Protein Family    Thrombospondin
 UniProt Gene Name    Thbs1 [Similar Products]
 UniProt Synonym Gene Names    Tsp1 [Similar Products]
 UniProt Entry Name    TSP1_MOUSE
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 NCBI Summary for THBS1    The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
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 UniProt Comments for THBS1    Function: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors.1 PublicationManual assertion based on experiment in:Ref.6
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 Research Articles on THBS1    1. the TSP1-CD36 tandem is another platelet ligand-receptor axis contributing to the maintenance of a stable thrombus.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with THBS1 elisa kitPathways associated with THBS1 elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with THBS1 and FLNA
 Products by Pathway  Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Inflammatory Response Pathway antibodies  Inflammatory Response Pathway Diagram
Organs/Tissues associated with THBS1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with THBS1 and Bone
 Connective Tissue Antibodies  >4 publications with THBS1 and Connective Tissue
 Heart Antibodies  >3 publications with THBS1 and Heart
 Muscle Antibodies  >3 publications with THBS1 and Muscle
 Brain Antibodies  >3 publications with THBS1 and Brain
 Vascular Antibodies  >2 publications with THBS1 and Vascular
 Embryonic Tissue Antibodies  >2 publications with THBS1 and Embryonic Tissue
 Ear Antibodies  >1 publications with THBS1 and Ear
 Kidney Antibodies  >1 publications with THBS1 and Kidney
 Umbilical Cord Antibodies  >1 publications with THBS1 and Umbilical Cord
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