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TRH elisa kit :: Mouse thyrotropin-releasing hormone ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700675
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $575 +1 FREE 8GB USB
96-Strip-Wells  /  $835 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

thyrotropin-releasing hormone (TRH), ELISA Kit

★Popular Item★
 Also Known As   

Mouse thyrotropin-releasing hormone, TRH ELISA Kit

 Product Synonym Names    Mouse thyrotropin-releasing hormone; TRH ELISA KIT; MGC125964; MGC125965; ; thyrotropin-releasing hormone
 Product Gene Name   

TRH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700675 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q62361
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse TRH. No significant cross-reactivity or interference between Mouse TRH and analogues was observed.
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 Samples    Serum
 Assay Type    Sandwich
 Detection Range    2.5 pg/ml-160 pg/ml
 Sensitivity    0.625 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TRH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TRH purchase    MBS700675 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the thyrotropin-releasing hormone (TRH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2.5 pg/ml-160 pg/ml in biological research samples containing TRH, with an estimated sensitivity of 0.625 pg/ml. The ELISA analytical biochemical technique of the MBS700675 kit is based on TRH antibody-TRH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TRH elisa kit

   For SampleFor the quantitative determination of mouse thyrotropin-releasing hormone (TRH) concentrations in serum.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRH bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of TRH elisa kit    TRH elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700675. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TRH. It may not necessarily be applicable to this product.
 NCBI GI #    134031937
 NCBI GeneID    22044
 NCBI Accession #    NP_033452.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_009426.3 [Other Products]
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 UniProt Primary Accession #    Q62361 [Other Products]
 UniProt Secondary Accession #    Q7TMT4 [Other Products]
 UniProt Related Accession #    Q62361 [Other Products]
 Molecular Weight    29,195 Da
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 NCBI Official Full Name    thyrotropin releasing hormone preproprotein
 NCBI Official Synonym Full Names    thyrotropin releasing hormone
 NCBI Official Symbol    Trh [Similar Products]
 NCBI Official Synonym Symbols   
Trf; Pro-TRH
[Similar Products]
 NCBI Protein Information    thyrotropin releasing hormone; protirelin; prothyroliberin; TSH-releasing factor; thyrotropin-releasing factor; pro-thyrotropin-releasing hormone
 UniProt Protein Name    Pro-thyrotropin-releasing hormone
 UniProt Synonym Protein Names   
ProthyroliberinCleaved into the following chain:Thyrotropin-releasing hormone; TRHAlternative name(s):Protirelin; TSH-releasing factor; Thyroliberin; Thyrotropin-releasing factor; TRF
 Protein Family    Thyrotropin-releasing hormone
 UniProt Gene Name    Trh [Similar Products]
 UniProt Synonym Gene Names    Pro-TRH; TRH; TRF [Similar Products]
 UniProt Entry Name    TRH_MOUSE
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 NCBI Summary for TRH    This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The mouse proprotein contains five thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Disruption of this gene results in hypothyroidism, elevated thyroid-stimulating hormone levels, and hyperglycemia. [provided by RefSeq, Apr 2013]
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 UniProt Comments for TRH    Function: Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.
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 Research Articles on TRH    1. RXRA and RXRB display specific roles in modulating T3-dependent regulation of thyrotropin releasing hormone.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TRH elisa kitPathways associated with TRH elisa kit
 Reference Product  PubMed Publications
 Prl elisa kit  >1755 publications with TRH and Prl
 Trhr elisa kit  >47 publications with TRH and Trhr
 Avp elisa kit  >45 publications with TRH and Avp
 Oxt elisa kit  >6 publications with TRH and Oxt
 Hcrt elisa kit  >2 publications with TRH and Hcrt
 Gnrhr elisa kit  >1 publications with TRH and Gnrhr
 Gnrh1 elisa kit  >1 publications with TRH and Gnrh1
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
Diseases associated with TRH elisa kitOrgans/Tissues associated with TRH elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2000 publications with TRH and Nervous System Diseases
 Brain Diseases Antibodies  >1683 publications with TRH and Brain Diseases
 Disease Models, Animal Antibodies  >133 publications with TRH and Disease Models, Animal
 Bipolar Disorder Antibodies  >102 publications with TRH and Bipolar Disorder
 Hypertension Antibodies  >98 publications with TRH and Hypertension
 Seizures Antibodies  >94 publications with TRH and Seizures
 Hypothermia Antibodies  >91 publications with TRH and Hypothermia
 Pain Antibodies  >86 publications with TRH and Pain
 Movement Disorders Antibodies  >60 publications with TRH and Movement Disorders
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with TRH and Bone
 Connective Tissue Antibodies  >4 publications with TRH and Connective Tissue
 Heart Antibodies  >3 publications with TRH and Heart
 Muscle Antibodies  >3 publications with TRH and Muscle
 Brain Antibodies  >3 publications with TRH and Brain
 Vascular Antibodies  >2 publications with TRH and Vascular
 Embryonic Tissue Antibodies  >2 publications with TRH and Embryonic Tissue
 Ear Antibodies  >1 publications with TRH and Ear
 Kidney Antibodies  >1 publications with TRH and Kidney
 Umbilical Cord Antibodies  >1 publications with TRH and Umbilical Cord
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