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TGFA elisa kit :: Mouse transforming growth factor, alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704708
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

transforming growth factor, alpha (TGFA), ELISA Kit

★Popular Item★
 Also Known As   

Mouse transforming growth factor alpha, TGF-alpha ELISA Kit

 Product Synonym Names    Mouse transforming growth factor alpha; TGF-alpha ELISA Kit; TFGA; transforming growth factor-alpha; transforming growth factor; alpha
 Product Gene Name   

TGFA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704708 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P48030
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse TGF-alpha. No significant cross-reactivity or interference between mouse TGF-alpha and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates and tissue homogenates
 Assay Type    Sandwich
 Detection Range    12.5 pg/ml -800 pg/ml.
 Sensitivity    The minimum detectable dose of mouse TGF-alpha is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TGFA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TGFA purchase    MBS704708 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the transforming growth factor, alpha (TGFA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 12.5 pg/ml -800 pg/ml in biological research samples containing TGFA, with an estimated sensitivity of The minimum detectable dose of mouse TGF-alpha is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS704708 kit is based on TGFA antibody-TGFA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGFA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGFA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates and tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for TGFA elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TGF-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of TGFA elisa kit    TGFA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704708. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TGFA. It may not necessarily be applicable to this product.
 NCBI GI #    13654266
 NCBI GeneID    21802
 NCBI Accession #    NP_112476.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_031199.3 [Other Products]
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 UniProt Primary Accession #    P48030 [Other Products]
 UniProt Related Accession #    P48030 [Other Products]
 Molecular Weight    17,018 Da
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 NCBI Official Full Name    protransforming growth factor alpha preproprotein
 NCBI Official Synonym Full Names    transforming growth factor alpha
 NCBI Official Symbol    Tgfa [Similar Products]
 NCBI Official Synonym Symbols   
wa1; wa-1
[Similar Products]
 NCBI Protein Information    protransforming growth factor alpha; waved 1; transforming growth factor-alpha
 UniProt Protein Name    Protransforming growth factor alpha
 UniProt Synonym Protein Names   
EGF-like TGF; ETGF; TGF type 1
 Protein Family    Transforming growth factor
 UniProt Gene Name    Tgfa [Similar Products]
 UniProt Synonym Gene Names    TGF-alpha; ETGF [Similar Products]
 UniProt Entry Name    TGFA_MOUSE
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 UniProt Comments for TGFA    Function: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
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 Research Articles on TGFA    1. findings suggest that elevated PHD4 levels disturb the angiogenic balance in osteosarcoma via induction of the TGF-alpha pathway and inhibit tumor growth by reducing the expression of HIF-2alpha
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TGFA elisa kitPathways associated with TGFA elisa kit
 Reference Product  PubMed Publications
 Egfr elisa kit  >41 publications with TGFA and Egfr
 Jun elisa kit  >25 publications with TGFA and Jun
 Tnf elisa kit  >7 publications with TGFA and Tnf
 Erbb2 elisa kit  >7 publications with TGFA and Erbb2
 Il6 elisa kit  >5 publications with TGFA and Il6
 Ccnd1 elisa kit  >3 publications with TGFA and Ccnd1
 Fos elisa kit  >2 publications with TGFA and Fos
 Hif1a elisa kit  >1 publications with TGFA and Hif1a
 Igf1r elisa kit  >1 publications with TGFA and Igf1r
 Products by Pathway  Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 Glioma Pathway antibodies  Glioma Pathway Diagram
 Glioma Pathway antibodies  Glioma Pathway Diagram
 Non-small Cell Lung Cancer Pathway antibodies  Non-small Cell Lung Cancer Pathway Diagram
 Non-small Cell Lung Cancer Pathway antibodies  Non-small Cell Lung Cancer Pathway Diagram
 Pancreatic Cancer Pathway antibodies  Pancreatic Cancer Pathway Diagram
 Pancreatic Cancer Pathway antibodies  Pancreatic Cancer Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
Diseases associated with TGFA elisa kitOrgans/Tissues associated with TGFA elisa kit
 Disease Name  Pubmed Publications
 Genetic Predisposition to Disease Antibodies  >19 publications with TGFA and Genetic Predisposition to Disease
 Fatty Liver Antibodies  >16 publications with TGFA and Fatty Liver
 Liver Diseases Antibodies  >10 publications with TGFA and Liver Diseases
 Liver Neoplasms Antibodies  >10 publications with TGFA and Liver Neoplasms
 Head and Neck Neoplasms Antibodies  >9 publications with TGFA and Head and Neck Neoplasms
 Breast Neoplasms Antibodies  >9 publications with TGFA and Breast Neoplasms
 Inflammation Antibodies  >7 publications with TGFA and Inflammation
 Nervous System Diseases Antibodies  >7 publications with TGFA and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >7 publications with TGFA and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >7 publications with TGFA and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with TGFA and Bone
 Connective Tissue Antibodies  >4 publications with TGFA and Connective Tissue
 Heart Antibodies  >3 publications with TGFA and Heart
 Muscle Antibodies  >3 publications with TGFA and Muscle
 Brain Antibodies  >3 publications with TGFA and Brain
 Vascular Antibodies  >2 publications with TGFA and Vascular
 Embryonic Tissue Antibodies  >2 publications with TGFA and Embryonic Tissue
 Ear Antibodies  >1 publications with TGFA and Ear
 Kidney Antibodies  >1 publications with TGFA and Kidney
 Umbilical Cord Antibodies  >1 publications with TGFA and Umbilical Cord
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