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TNNI3 elisa kit :: Mouse troponin I type 3 (cardiac) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703536
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $575 +1 FREE 8GB USB
96-Strip-Wells  /  $835 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
 
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 Product Name   

troponin I type 3 (cardiac) (TNNI3), ELISA Kit

★Popular Item★
 Also Known As   

Mouse cardiac troponin I , cTn-I  ELISA Kit

 Product Synonym Names    Mouse cardiac troponin I (cTn-I) ELISA kit; CMD2A; CMH7; MGC116817; RCM1; TNNC1; cTnI; familial hypertrophic cardiomyopathy 7; troponin I; cardiac; troponin I type 3 (cardiac)
 Product Gene Name   

TNNI3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703536 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q6FGX2
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse cTn-I. No significant cross-reactivity or interference between mouse cTn-I and analogues was observed.
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 Samples    Serum, plasma
 Detection Range    15.6 pg/ml -1000 pg/mL
 Sensitivity    <3.9 pg/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TNNI3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TNNI3 purchase    MBS703536 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the troponin I type 3 (cardiac) (TNNI3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml -1000 pg/mL in biological research samples containing TNNI3, with an estimated sensitivity of <3.9 pg/mL. The ELISA analytical biochemical technique of the MBS703536 kit is based on TNNI3 antibody-TNNI3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNNI3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNNI3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TNNI3 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for cTn-I has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cTn-I present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cTn-I is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cTn-I bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of TNNI3 elisa kit    TNNI3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703536. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TNNI3. It may not necessarily be applicable to this product.
 NCBI GI #    49456923
 NCBI GeneID    7137
 NCBI Accession #    CAG46782.1 [Other Products]
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 UniProt Primary Accession #    Q6FGX2 [Other Products]
 UniProt Related Accession #    P19429 [Other Products]
 Molecular Weight    24,008 Da
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 NCBI Official Full Name    TNNI3
 NCBI Official Synonym Full Names    troponin I type 3 (cardiac)
 NCBI Official Symbol    TNNI3 [Similar Products]
 NCBI Official Synonym Symbols   
CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
[Similar Products]
 NCBI Protein Information    troponin I, cardiac muscle; cardiomyopathy, dilated 2A (autosomal recessive)
 UniProt Protein Name    TNNI3 protein
 UniProt Synonym Protein Names   
TNNI3 protein; Troponin I type 3 (Cardiac); Troponin I, cardiac; Troponin I, cardiac variant
 Protein Family    Troponin
 UniProt Gene Name    TNNI3 [Similar Products]
 UniProt Entry Name    Q6FGX2_HUMAN
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 NCBI Summary for TNNI3    Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
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 UniProt Comments for TNNI3    TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Motor; Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: sarcomere; troponin complex; cytosol

Molecular Function: troponin T binding; troponin C binding; protein domain specific binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; calcium-dependent protein binding; protein kinase binding

Biological Process: cellular calcium ion homeostasis; regulation of smooth muscle contraction; heart contraction; regulation of systemic arterial blood pressure by ischemic conditions; heart development; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; cardiac muscle contraction; muscle filament sliding

Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a
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 Research Articles on TNNI3    1. Cardiac TnI release is common before Norwood and BDG operations; then during the treatment protocol for hypoplastic left heart syndrome, cTnI release resolves.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TNNI3 elisa kitPathways associated with TNNI3 elisa kit
 Reference Product  PubMed Publications
 TNNT2 elisa kit  >38 publications with TNNI3 and TNNT2
 TNNC1 elisa kit  >11 publications with TNNI3 and TNNC1
 TNNI3K elisa kit  >5 publications with TNNI3 and TNNI3K
 RCAN3 elisa kit  >2 publications with TNNI3 and RCAN3
 ACTA1 elisa kit  >1 publications with TNNI3 and ACTA1
 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
Diseases associated with TNNI3 elisa kitOrgans/Tissues associated with TNNI3 elisa kit
 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >71 publications with TNNI3 and Heart Diseases
 Cardiomyopathies Antibodies  >63 publications with TNNI3 and Cardiomyopathies
 Cardiomyopathy, Hypertrophic, Familial Antibodies  >25 publications with TNNI3 and Cardiomyopathy, Hypertrophic, Familial
 Cardiomyopathy, Dilated Antibodies  >19 publications with TNNI3 and Cardiomyopathy, Dilated
 CARDIOMYOPATHY, FAMILIAL HYPERTROPHIC, 7 Antibodies  >9 publications with TNNI3 and CARDIOMYOPATHY, FAMILIAL HYPERTROPHIC, 7
 Cardiomyopathy, Restrictive Antibodies  >8 publications with TNNI3 and Cardiomyopathy, Restrictive
 Nervous System Diseases Antibodies  >5 publications with TNNI3 and Nervous System Diseases
 Hypertension Antibodies  >4 publications with TNNI3 and Hypertension
 Cardiomyopathy, Familial Restrictive, 1 Antibodies  >3 publications with TNNI3 and Cardiomyopathy, Familial Restrictive, 1
 Disease Models, Animal Antibodies  >2 publications with TNNI3 and Disease Models, Animal
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >44 publications with TNNI3 and Heart
 Lung Antibodies  >2 publications with TNNI3 and Lung
 Uterus Antibodies  >1 publications with TNNI3 and Uterus
 Embryonic Tissue Antibodies  >1 publications with TNNI3 and Embryonic Tissue
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