|
| Product Name
|
|
tryptase beta 2 (TPSB2), ELISA Kit ★Popular Item★
|
| Also Known As
|
|
Mouse Tryptase beta-2, TPSB2 ELISA Kit
|
| Product Synonym Names
|
|
Mouse Tryptase beta-2 (TPSB2) ELISA kit; TPS2; tryptaseB; tryptaseC; OTTHUMP00000208944; mast cell tryptase beta II; mast cell tryptase beta III; tryptase II; tryptase III; tryptase beta 2; tryptase beta II; tryptase beta III; tryptase beta 2
|
| Product Gene Name
|
|
|
[Similar Products]
|
| Research Use Only
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
 TOP
|
| Sample Manual Insert
|
|
 
|
| Request for Current Manual Insert
|
|
|
| MBS945041 Testing Data
|
|
|
|
| 3D Structure
|
|
ModBase 3D Structure for P21845
|
| Species Reactivity
|
|
Mouse
|
|
| Specificity
|
|
This assay has high sensitivity and excellent specificity for detection of mouse TPSB2. No significant cross-reactivity or interference between mouse TPSB2 and analogues was observed.
|
|
|
| Samples
|
|
Serum, plasma, tissue homogenates
|
| Assay Type
|
|
Sandwich
|
| Detection Range
|
|
78 pg/ml -5000 pg/ml.
|
| Sensitivity
|
|
The minimum detectable dose of mouse TPSB2 is typically less than 19.5 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
|
| Intra-assay Precision
|
|
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
|
| Inter-assay Precision
|
|
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
|
|
|
| Preparation and Storage
|
|
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
|
| ISO Certification
|
|
Manufactured in an ISO 9001:2008 Certified Laboratory.
|
| Product Note
|
|
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
|
| Other Notes
|
|
Small volumes of TPSB2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
|
| Searchable Terms for TPSB2 purchase
|
|
MBS945041 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tryptase beta 2 (TPSB2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78 pg/ml -5000 pg/ml in biological research samples containing TPSB2, with an estimated sensitivity of The minimum detectable dose of mouse TPSB2 is typically less than 19.5 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS945041 kit is based on TPSB2 antibody-TPSB2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPSB2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPSB2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
|
|
TOP
|
|
Related Product Information for TPSB2 elisa kit
|
|
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPSB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPSB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPSB2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPSB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
|
|
|
|
| Typical Testing Data/Standard Curve (for reference only) of TPSB2 elisa kit
|
|
|
|
TOP
|
|
| Sample Manual Insert of MBS945041. Click to request current manual
|
|
|
|
TOP
|
|
| NCBI/Uniprot data below describe general gene information for TPSB2. It may not necessarily be applicable to this product. |
| NCBI GI #
|
|
126839
|
| NCBI Accession #
|
|
P21845.2
|
[Other Products]
|
|
|
| UniProt Primary Accession #
|
|
P21845
|
[Other Products]
|
| UniProt Secondary Accession #
|
|
Q61962
|
[Other Products]
|
| Molecular Weight
|
|
30,927 Da
|
|
TOP
|
| NCBI Official Full Name
|
|
Tryptase beta-2
|
| UniProt Protein Name
|
|
Tryptase beta-2
|
| UniProt Synonym Protein Names
|
|
Mast cell protease 6; mMCP-6
|
| Protein Family
|
|
Tryptase
|
| UniProt Gene Name
|
|
Tpsb2
|
[Similar Products]
|
| UniProt Synonym Gene Names
|
|
Mcpt6; Tryptase-2; mMCP-6
|
[Similar Products]
|
| UniProt Entry Name
|
|
TRYB2_MOUSE
|
|
|
| UniProt Comments for TPSB2
|
|
TPSB2: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection. Belongs to the peptidase S1 family. Tryptase subfamily.
Protein type: Protease; Secreted; Secreted, signal peptide; EC 3.4.21.59
Cellular Component: extracellular matrix; extracellular space; extracellular region
Molecular Function: peptidase activity; heparin binding; protein binding; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity
Biological Process: proteolysis; inflammatory response
|
|
TOP
|
| Precautions
|
|
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
|
| Disclaimer
|
|
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
|
|
TOP
|
|
| Products associated with TPSB2 elisa kit | Pathways associated with TPSB2 elisa kit |
|
|
|
|
|
|
TOP
|
ELISA Kit 
