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TNFSF15 elisa kit :: Mouse tumor necrosis factor (ligand) superfamily, member 15 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705929
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

tumor necrosis factor (ligand) superfamily, member 15 (TNFSF15), ELISA Kit

★Popular Item★
 Also Known As   

Mouse tumor necrosis factor-like ligand 1, TL1 ELISA Kit

 Product Synonym Names    Mouse tumor necrosis factor-like ligand 1 (TL1) ELISA kit; MGC129934; MGC129935; TL1; TL1A; VEGI; VEGI192A; TNF ligand-related molecule 1; TNF superfamily ligand TL1A; vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor-192A; tumor necrosis factor (ligand) superfamily; member 15
 Product Gene Name   

TNFSF15 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705929 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q5UBV8
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse TL1. No significant cross-reactivity or interference between mouse TL1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates.
 Assay Type    Sandwich
 Detection Range    62.5 pg/ml-4000 pg/ml.
 Sensitivity    Typically less than 15.6 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TNFSF15 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TNFSF15 purchase    MBS705929 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tumor necrosis factor (ligand) superfamily, member 15 (TNFSF15) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 pg/ml-4000 pg/ml in biological research samples containing TNFSF15, with an estimated sensitivity of Typically less than 15.6 pg/ml. The ELISA analytical biochemical technique of the MBS705929 kit is based on TNFSF15 antibody-TNFSF15 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNFSF15 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNFSF15. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). This product is a highly sensitive sandwich ELISA assay kit for detecting and quantifying mouse tumor necrosis factor ligand superfamily member 15 (TNFSF15). In this indirect mouse tumor necrosis factor-like ligand 1 (TL1) ELISA assay, known amounts of anti-TL1 antibodies are adsorbed to each plate well. A sample containing TL1 is added to each well and binds to capture antibodies. Unbound antigen is washed away. Subsequently, a detection antibody solution containing biotin conjugated anti TL1 antibodies, forming a complex with the previously bound TL1 and anti TL1 capture antibodies. Another wash removes unbound biotin conjugated antibodies, and avidin conjugated with horseradish peroxidase is added. A final wash removes any unbound avidin. Finally, a chromogenic substrate is added. The amount of bound enzyme varies directly with the concentration of protein in the original test sample, thus the absorbance at 450nm is inversely proportional with the concentration of mouse tumor necrosis factor-like ligand 1 added to each well. The concentration of TL1 in the test sample can be derived from a calibration curve of known standards.
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Related Product Information for TNFSF15 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of TNFSF15 elisa kit    TNFSF15 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705929. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TNFSF15. It may not necessarily be applicable to this product.
 NCBI GI #    238776819
 NCBI GeneID    326623
 NCBI Accession #    NP_796345.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_177371.3 [Other Products]
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 UniProt Primary Accession #    Q5UBV8 [Other Products]
 UniProt Secondary Accession #    Q8K3Y8 [Other Products]
 UniProt Related Accession #    Q5UBV8 [Other Products]
 Molecular Weight    27,723 Da
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 NCBI Official Full Name    tumor necrosis factor ligand superfamily member 15
 NCBI Official Synonym Full Names    tumor necrosis factor (ligand) superfamily, member 15
 NCBI Official Symbol    Tnfsf15 [Similar Products]
 NCBI Official Synonym Symbols   
Tl1; Tl1a; Vegi
[Similar Products]
 NCBI Protein Information    tumor necrosis factor ligand superfamily member 15; TNF ligand-related molecule 1; vascular endothelial growth inhibitor; vascular endothelial cell growth inhibitor; bM20K13.3 (tumor necrosis factor (ligand) superfamily, member 15)
 UniProt Protein Name    Tumor necrosis factor ligand superfamily member 15
 UniProt Synonym Protein Names   
TNF ligand-related molecule 1; Vascular endothelial cell growth inhibitor
 Protein Family    Tumor necrosis factor ligand superfamily
 UniProt Gene Name    Tnfsf15 [Similar Products]
 UniProt Synonym Gene Names    Tl1; Vegi [Similar Products]
 UniProt Entry Name    TNF15_MOUSE
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 UniProt Comments for TNFSF15    Function: Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis Promotes splenocyte alloactivation.1 PublicationManual assertion based on experiment in:Ref.1
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 Research Articles on TNFSF15    1. delivers survival signals to plasma cells and promotes pathogenic Ab production in collagen arthritis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TNFSF15 elisa kitPathways associated with TNFSF15 elisa kit
 Reference Product  PubMed Publications
 Tnfrsf25 elisa kit  >33 publications with TNFSF15 and Tnfrsf25
 Ccdc122 elisa kit  >2 publications with TNFSF15 and Ccdc122
 Tnfrsf11b elisa kit  >1 publications with TNFSF15 and Tnfrsf11b
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
Diseases associated with TNFSF15 elisa kitOrgans/Tissues associated with TNFSF15 elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >159 publications with TNFSF15 and Necrosis
 Colitis, Ulcerative Antibodies  >26 publications with TNFSF15 and Colitis, Ulcerative
 Disease Models, Animal Antibodies  >10 publications with TNFSF15 and Disease Models, Animal
 Skin Diseases Antibodies  >7 publications with TNFSF15 and Skin Diseases
 Atherosclerosis Antibodies  >6 publications with TNFSF15 and Atherosclerosis
 Liver Diseases Antibodies  >6 publications with TNFSF15 and Liver Diseases
 Cardiovascular Diseases Antibodies  >5 publications with TNFSF15 and Cardiovascular Diseases
 Fibrosis Antibodies  >5 publications with TNFSF15 and Fibrosis
 Neoplasms, Experimental Antibodies  >4 publications with TNFSF15 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >4 publications with TNFSF15 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >66 publications with TNFSF15 and Blood
 Intestine Antibodies  >12 publications with TNFSF15 and Intestine
 Embryonic Tissue Antibodies  >10 publications with TNFSF15 and Embryonic Tissue
 Lung Antibodies  >6 publications with TNFSF15 and Lung
 Kidney Antibodies  >4 publications with TNFSF15 and Kidney
 Brain Antibodies  >2 publications with TNFSF15 and Brain
 Eye Antibodies  >2 publications with TNFSF15 and Eye
 Testis Antibodies  >1 publications with TNFSF15 and Testis
 Prostate Antibodies  >1 publications with TNFSF15 and Prostate
 Thyroid Antibodies  >1 publications with TNFSF15 and Thyroid
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