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WNT5A elisa kit :: Mouse wingless-type MMTV integration site family, member 5A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS932852
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

wingless-type MMTV integration site family, member 5A (WNT5A), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Protein Wnt-5a, WNT5A ELISA Kit

 Product Synonym Names    Mouse Protein Wnt-5a (WNT5A) ELISA kit; hWNT5A; WNT-5A protein; wingless-type MMTV integration site family; member 5A
 Product Gene Name   

WNT5A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS932852 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P22725
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse WNT5A. No significant cross-reactivity or interference between mouse WNT5A and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates
 Assay Type    Sandwich
 Detection Range    3.12 pg/ml -200 pg/ml.
 Sensitivity    The minimum detectable dose of mouse WNT5A is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of WNT5A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for WNT5A purchase    MBS932852 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the wingless-type MMTV integration site family, member 5A (WNT5A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12 pg/ml -200 pg/ml in biological research samples containing WNT5A, with an estimated sensitivity of The minimum detectable dose of mouse WNT5A is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS932852 kit is based on WNT5A antibody-WNT5A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT5A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT5A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for WNT5A elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT5A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT5A bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of WNT5A elisa kit    WNT5A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS932852. Click to request current manual
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NCBI/Uniprot data below describe general gene information for WNT5A. It may not necessarily be applicable to this product.
 NCBI GI #    371940979
 NCBI GeneID    22418
 NCBI Accession #    NP_001243153.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001256224.1 [Other Products]
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 UniProt Primary Accession #    P22725 [Other Products]
 UniProt Secondary Accession #    Q8BM17; Q8BMF9; Q8VCV6 [Other Products]
 UniProt Related Accession #    P22725 [Other Products]
 Molecular Weight    42,309 Da
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 NCBI Official Full Name    protein Wnt-5a isoform 2
 NCBI Official Synonym Full Names    wingless-type MMTV integration site family, member 5A
 NCBI Official Symbol    Wnt5a [Similar Products]
 NCBI Official Synonym Symbols   
Wnt-5a; 8030457G12Rik
[Similar Products]
 NCBI Protein Information    protein Wnt-5a; wingless-related MMTV integration site 5A
 UniProt Protein Name    Protein Wnt-5a
 Protein Family    Protein
 UniProt Gene Name    Wnt5a [Similar Products]
 UniProt Synonym Gene Names    Wnt-5a [Similar Products]
 UniProt Entry Name    WNT5A_MOUSE
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 UniProt Comments for WNT5A    WNT5A: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. Interacts with PORCN. Interacts with WLS. Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; cytoplasm; extracellular region; intracellular

Molecular Function: protein domain specific binding; receptor tyrosine kinase-like orphan receptor binding; protein binding; frizzled binding; cytokine activity; transcription factor activity; frizzled-2 binding; receptor binding

Biological Process: neural tube development; positive regulation of JNK activity; activation of MAPK activity; multicellular organismal development; embryonic skeletal development; positive regulation of transcription, DNA-dependent; negative chemotaxis; positive regulation of endocytosis; Wnt receptor signaling pathway through beta-catenin; positive regulation of interleukin-1 beta secretion; negative regulation of synaptogenesis; uterus development; protein amino acid phosphorylation; negative regulation of BMP signaling pathway; activation of NF-kappaB transcription factor; anterior/posterior pattern formation; positive regulation of fibroblast proliferation; regulation of protein localization; cell-cell signaling; convergent extension; determination of anterior/posterior axis, embryo; positive regulation of mesenchymal cell proliferation; JNK cascade; embryonic limb morphogenesis; positive regulation of macrophage activation; positive regulation of cytokine production; somitogenesis; inner ear morphogenesis; cell fate commitment; urinary bladder development; olfactory bulb interneuron development; activation of protein kinase B; positive regulation of interleukin-6 production; vagina development; digestive tract morphogenesis; negative regulation of fat cell differentiation; genitalia development; organ morphogenesis; positive regulation of angiogenesis; Wnt receptor signaling pathway, calcium modulating pathway; positive regulation of protein catabolic process; midgut development; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; determination of left/right symmetry; negative regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; axon guidance; wound healing; negative regulation of fibroblast growth factor receptor signaling pathway; hypophysis morphogenesis; positive regulation of cytokine secretion during immune response; positive regulation of JNK cascade; palate development; negative regulation of axon extension involved in axon guidance; signal transduction; activation of JNK activity; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; morphogenesis of an epithelium; establishment of planar polarity; heart looping; cell differentiation; negative regulation of epithelial cell proliferation; cervix development; cell migration; Wnt receptor signaling pathway; positive regulation of meiosis; Wnt receptor signaling pathway, planar cell polarity pathway; male gonad development; positive regulation of peptidyl-serine phosphorylation; limb morphogenesis; positive regulation of ossification; positive regulation of cGMP metabolic process; development of primary male sexual characteristics; convergent extension involved in organogenesis; embryonic development; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; cartilage development; ameboidal cell migration; neural tube closure; hindgut morphogenesis; positive regulation of protein amino acid phosphorylation; neurite morphogenesis; positive regulation of inflammatory response; lung development
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 Research Articles on WNT5A    1. recombinant Wnt3a- and Wnt5a-induced cytokine production from murine C57BL/6 macrophages was dependent on TLR4 and inhibited by Polymyxin B.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with WNT5A elisa kitPathways associated with WNT5A elisa kit
 Reference Product  PubMed Publications
 Porcn elisa kit  >2 publications with WNT5A and Porcn
 Products by Pathway  Pathway Diagram
 Asymmetric Localization Of PCP Proteins Pathway antibodies  Asymmetric Localization Of PCP Proteins Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Ca2+ Pathway antibodies  Ca2+ Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 ESC Pluripotency Pathways antibodies  ESC Pluripotency Pathways Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
Diseases associated with WNT5A elisa kitOrgans/Tissues associated with WNT5A elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >55 publications with WNT5A and Breast Neoplasms
 Inflammation Antibodies  >53 publications with WNT5A and Inflammation
 Neoplasm Metastasis Antibodies  >51 publications with WNT5A and Neoplasm Metastasis
 Adenocarcinoma Antibodies  >43 publications with WNT5A and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >40 publications with WNT5A and Neoplasms, Experimental
 Female Urogenital Diseases Antibodies  >37 publications with WNT5A and Female Urogenital Diseases
 Nervous System Diseases Antibodies  >29 publications with WNT5A and Nervous System Diseases
 Skin Neoplasms Antibodies  >26 publications with WNT5A and Skin Neoplasms
 Lung Diseases Antibodies  >24 publications with WNT5A and Lung Diseases
 Cell Transformation, Neoplastic Antibodies  >23 publications with WNT5A and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >112 publications with WNT5A and Embryonic Tissue
 Brain Antibodies  >67 publications with WNT5A and Brain
 Skin Antibodies  >53 publications with WNT5A and Skin
 Lung Antibodies  >37 publications with WNT5A and Lung
 Liver Antibodies  >24 publications with WNT5A and Liver
 Heart Antibodies  >24 publications with WNT5A and Heart
 Intestine Antibodies  >24 publications with WNT5A and Intestine
 Stomach Antibodies  >19 publications with WNT5A and Stomach
 Mammary Gland Antibodies  >18 publications with WNT5A and Mammary Gland
 Eye Antibodies  >16 publications with WNT5A and Eye
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