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XDH elisa kit :: Mouse xanthine dehydrogenase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS918183
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
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 Product Name   

xanthine dehydrogenase (XDH), ELISA Kit

 Also Known As   

Mouse Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase, XDH ELISA Kit

 Product Synonym Names    Mouse Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase (XDH) ELISA kit; XO; XOR; xanthene dehydrogenase; xanthine oxidase; xanthine oxidoreductase; xanthine dehydrogenase
 Product Gene Name   

XDH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS918183 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q00519
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse XDH. No significant cross-reactivity or interference between mouse XDH and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates.
 Assay Type    Sandwich
 Detection Range    7.8 pg/ml -500 pg/ml.
 Sensitivity    1.95 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of XDH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for XDH purchase    MBS918183 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the xanthine dehydrogenase (XDH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.8 pg/ml -500 pg/ml in biological research samples containing XDH, with an estimated sensitivity of 1.95 pg/ml. The ELISA analytical biochemical technique of the MBS918183 kit is based on XDH antibody-XDH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect XDH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, XDH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for XDH elisa kit

   Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for XDH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any XDH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XDH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XDH bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of XDH elisa kit    XDH elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS918183. Click to request current manual
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NCBI/Uniprot data below describe general gene information for XDH. It may not necessarily be applicable to this product.
 NCBI GI #    77682555
 NCBI GeneID    22436
 NCBI Accession #    NP_035853.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_011723.3 [Other Products]
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 UniProt Primary Accession #    Q00519 [Other Products]
 UniProt Secondary Accession #    E9QLM9 [Other Products]
 UniProt Related Accession #    Q00519 [Other Products]
 Molecular Weight    146,562 Da
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 NCBI Official Full Name    xanthine dehydrogenase/oxidase
 NCBI Official Synonym Full Names    xanthine dehydrogenase
 NCBI Official Symbol    Xdh [Similar Products]
 NCBI Official Synonym Symbols   
XO; Xor; Xox1; Xox-1
[Similar Products]
 NCBI Protein Information    xanthine dehydrogenase/oxidase; xanthine oxidase; xanthine oxidoreductase
 UniProt Protein Name    Xanthine dehydrogenase/oxidase
 UniProt Synonym Protein Names   
Xanthine oxidoreductase; XOR
 Protein Family    Xanthine dehydrogenase
 UniProt Gene Name    Xdh [Similar Products]
 UniProt Synonym Gene Names    XD; XO; XOR [Similar Products]
 UniProt Entry Name    XDH_MOUSE
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 NCBI Summary for XDH    This gene encodes a member of the xanthine dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein exists as two distinct enzymatic forms, either as xanthine dehydrogenase, or as xanthine oxidase, and functions in purine degradation. Additional studies also suggest a role in adipogenesis, and a function as a structural protein in milk fat droplets in the lactating mammary gland. [provided by RefSeq, Jan 2014]
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 UniProt Comments for XDH    XDH: Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). Defects in XDH are the cause of xanthinuria type 1 (XU1). Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol. Belongs to the xanthine dehydrogenase family.

Protein type: Secondary Metabolites Metabolism - caffeine; EC; Xenobiotic Metabolism - drug metabolism - other enzymes; Oxidoreductase; Nucleotide Metabolism - purine; EC

Cellular Component: extracellular space; sarcoplasmic reticulum; cytoplasm; extracellular region; peroxisome; cytosol

Molecular Function: 2 iron, 2 sulfur cluster binding; protein homodimerization activity; FAD binding; molybdenum ion binding; metal ion binding; iron-sulfur cluster binding; oxidoreductase activity; xanthine oxidase activity; oxidoreductase activity, acting on CH-OH group of donors; molybdopterin cofactor binding; UDP-N-acetylmuramate dehydrogenase activity; protein binding; oxidoreductase activity, acting on the aldehyde or oxo group of donors; electron carrier activity; iron ion binding; xanthine dehydrogenase activity; catalytic activity

Biological Process: caspase activation; lactation; xanthine catabolic process; negative regulation of protein kinase B signaling cascade; negative regulation of protein amino acid phosphorylation; negative regulation of endothelial cell differentiation; regulation of epithelial cell differentiation; negative regulation of endothelial cell proliferation; response to aluminum ion; bone resorption
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 Research Articles on XDH    1. The expression and the activity of XO were increased dramatically within the central nervous system of experimental autoimmune encephalomyelitis mice as compared to naive mice.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with XDH elisa kitDiseases associated with XDH elisa kit
 Products by Pathway  Pathway Diagram
 Caffeine Metabolism Pathway antibodies  Caffeine Metabolism Pathway Diagram
 Caffeine Metabolism Pathway antibodies  Caffeine Metabolism Pathway Diagram
 Drug Metabolism - Other Enzymes Pathway antibodies  Drug Metabolism - Other Enzymes Pathway Diagram
 Drug Metabolism - Other Enzymes Pathway antibodies  Drug Metabolism - Other Enzymes Pathway Diagram
 Folic Acid Network Pathway antibodies  Folic Acid Network Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Peroxisome Pathway antibodies  Peroxisome Pathway Diagram
 Peroxisome Pathway antibodies  Peroxisome Pathway Diagram
 Purine Catabolism Pathway antibodies  Purine Catabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Reperfusion Injury Antibodies  >26 publications with XDH and Reperfusion Injury
 Cardiovascular Diseases Antibodies  >24 publications with XDH and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >16 publications with XDH and Disease Models, Animal
 Neoplasms Antibodies  >13 publications with XDH and Neoplasms
 Kidney Diseases Antibodies  >12 publications with XDH and Kidney Diseases
 Anoxia Antibodies  >11 publications with XDH and Anoxia
 Inflammation Antibodies  >10 publications with XDH and Inflammation
 Nervous System Diseases Antibodies  >10 publications with XDH and Nervous System Diseases
 XANTHINURIA, TYPE I Antibodies  >9 publications with XDH and XANTHINURIA, TYPE I
 Drug Toxicity Antibodies  >8 publications with XDH and Drug Toxicity
Organs/Tissues associated with XDH elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >75 publications with XDH and Liver
 Lung Antibodies  >21 publications with XDH and Lung
 Intestine Antibodies  >15 publications with XDH and Intestine
 Skin Antibodies  >11 publications with XDH and Skin
 Eye Antibodies  >10 publications with XDH and Eye
 Brain Antibodies  >10 publications with XDH and Brain
 Mammary Gland Antibodies  >6 publications with XDH and Mammary Gland
 Bone Antibodies  >4 publications with XDH and Bone
 Embryonic Tissue Antibodies  >4 publications with XDH and Embryonic Tissue
 Bone Marrow Antibodies  >4 publications with XDH and Bone Marrow
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